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. 1980 Sep;66(3):505–509. doi: 10.1104/pp.66.3.505

Evidence for Light-stimulated Synthesis of Phosphoenolpyruvate Carboxylase in Leaves of Maize 1

Donald R Hague 1, Thomas L Sims 1
PMCID: PMC440662  PMID: 16661464

Abstract

Illumination (22,000 lumens per meter2) of etiolated maize plants for 80 hours brings about a 5-fold increase in phosphoenolpyruvate carboxylase activity per unit of protein. An increase in carboxylase protein and incorporation of [35S]methionine into the protein occurs simultaneously with the activity increase. In green plants, the level of phosphoenolpyruvate carboxylase protein and enzyme activity is dependent on the intensity of light during growth. These results are consistent with the conclusion that the activity increase results from light-stimulated de novo synthesis of phosphoenolypyruvate carboxylase protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bownes M., Hames B. D. Accumulation and degradation of three major yolk proteins in Drosophila melanogaster. J Exp Zool. 1977 Apr;200(1):149–156. doi: 10.1002/jez.1402000118. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  4. Edwards G. E. Isolation of Intact and Functional Chloroplasts from Mesophyll and Bundle Sheath Protoplasts of the C(4) Plant Panicum miliaceum. Plant Physiol. 1979 May;63(5):821–827. doi: 10.1104/pp.63.5.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kanai R., Edwards G. E. Separation of mesophyll protoplasts and bundle sheath cells from maize leaves for photosynthetic studies. Plant Physiol. 1973 Jun;51(6):1133–1137. doi: 10.1104/pp.51.6.1133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Loomis W. D. Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Methods Enzymol. 1974;31:528–544. doi: 10.1016/0076-6879(74)31057-9. [DOI] [PubMed] [Google Scholar]
  7. Mukerji S. K. Corn leaf phosphoenolpyruvate carboxylases. Purification and properties of two isoenzymes. Arch Biochem Biophys. 1977 Jul;182(1):343–351. doi: 10.1016/0003-9861(77)90315-0. [DOI] [PubMed] [Google Scholar]
  8. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  9. Ting I. P., Osmond C. B. Multiple forms of plant phosphoenolpyruvate carboxylase associated with different metabolic pathways. Plant Physiol. 1973 Mar;51(3):448–453. doi: 10.1104/pp.51.3.448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Uedan K., Sugiyama T. Purification and characterization of phosphoenolpyruvate carboxylase from maize leaves. Plant Physiol. 1976 Jun;57(6):906–910. doi: 10.1104/pp.57.6.906. [DOI] [PMC free article] [PubMed] [Google Scholar]

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