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. 1980 Oct;66(4):550–554. doi: 10.1104/pp.66.4.550

Corn Root Protoplasts

ISOLATION AND GENERAL CHARACTERIZATION OF ION TRANSPORT 1, 2

Willy Lin 1
PMCID: PMC440676  PMID: 16661475

Abstract

A method was developed for the large scale and rapid isolation of intact viable corn root protoplasts. Pure and metabolically active protoplasts were collected using a flotation technique. Vital staining tests, light and electron microscopy, and measurements of basic metabolic processes indicated that the isolated protoplasts were metabolically active, and that the plasmalemma and other organelles were well preserved. The isolated protoplasts performed normal, active ion transport functions. Time course of K+ and inorganic phosphate (H2PO4−) influx and the effects of external pH, carbonyl cyanide p-trifluoromethoxyphenylhydrazone, fusicoccin, and diethylstilbestrol on K+ and inorganic phosphate influx and net H+ efflux in isolated protoplasts correlated well with data obtained on root segments. Data presented indicated that isolated protoplasts from roots can be used to gain additional insights into the mechanism of ion transport in plant cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butcher H. C., Wagner G. J., Siegelman H. W. Localization of Acid hydrolases in protoplasts: examination of the proposed lysosomal function of the mature vacuole. Plant Physiol. 1977 Jun;59(6):1098–1103. doi: 10.1104/pp.59.6.1098. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cocking E. C. The isolation of plant protoplasts. Methods Enzymol. 1974;31:578–583. doi: 10.1016/0076-6879(74)31063-4. [DOI] [PubMed] [Google Scholar]
  3. Cocking E. C. Uptake of foreign genetic material by plant protoplasts. Int Rev Cytol. 1977;48:323–343. doi: 10.1016/s0074-7696(08)61748-9. [DOI] [PubMed] [Google Scholar]
  4. Guy M., Reinhold L. Membrane transport of sugars and amino acids in isolated protoplasts. Plant Physiol. 1978 Apr;61(4):593–596. doi: 10.1104/pp.61.4.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Higinbotham N. Conceptual developments in membrane transport, 1924-1974. Plant Physiol. 1974 Oct;54(4):454–462. doi: 10.1104/pp.54.4.454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lin W. Potassium and Phosphate Uptake in Corn Roots: Further Evidence for an Electrogenic H/K Exchanger and an OH/Pi Antiporter. Plant Physiol. 1979 May;63(5):952–955. doi: 10.1104/pp.63.5.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lin W., Wagner G. J., Siegelman H. W., Hind G. Membrane-bound ATPase of intact vacuoles and tonoplasts isolated from mature plant tissue. Biochim Biophys Acta. 1977 Feb 14;465(1):110–117. doi: 10.1016/0005-2736(77)90359-5. [DOI] [PubMed] [Google Scholar]
  8. Mettler I. J., Leonard R. T. Ion Transport in Isolated Protoplasts from Tobacco Suspension Cells: II. Selectivity and Kinetics. Plant Physiol. 1979 Jan;63(1):191–194. doi: 10.1104/pp.63.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mettler I. J., Leonard R. T. Ion transport in isolated protoplasts from tobacco suspension cells: I. General characteristics. Plant Physiol. 1979 Jan;63(1):183–190. doi: 10.1104/pp.63.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nishimura M., Beevers H. Hydrolases in vacuoles from castor bean endosperm. Plant Physiol. 1978 Jul;62(1):44–48. doi: 10.1104/pp.62.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nishimura M., Beevers H. Isolation of intact plastids from protoplasts from castor bean endosperm. Plant Physiol. 1978 Jul;62(1):40–43. doi: 10.1104/pp.62.1.40. [DOI] [PMC free article] [PubMed] [Google Scholar]

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