Figure 4. Environmental PPARγ ligands induce perilipin protein expression in mouse BM–MSCs.

Primary bone marrow cultures were established from male C57BL/6J mice and treated with Vh (DMSO), rosiglitazone (Rosi, 100 nM), TBT (100 nM), TPhT (50 nM), MEHP, TBBPA, or METBP (20 µM) in the presence of osteoinductive media. Cells were harvested after 7 days. Perilipin and β-actin expression were determined in whole cell lysates by immunoblot. Image is representative of 4 separate experiments.