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. 1980 Oct;66(4):688–691. doi: 10.1104/pp.66.4.688

Biochemical Characterization of a Catalase Inhibitor from Maize 1

John C Sorenson 1, John G Scandalios 1
PMCID: PMC440704  PMID: 16661503

Abstract

Some biochemical properties of the catalase inhibitor purified from maize scutella are described. The inhibitor is heat-labile and its activity is destroyed by trypsin, indicating that it is a protein. It does not appear to be a lectin nor does the inhibition involve proteolysis. The active inhibitor is a dimer with each subunit having a molecular weight of 5600 as determined by sodium dodecyl sulfate electrophoresis. A kinetic analysis performed in the presence of increasing levels of inhibitor gave unusual Lineweaver-Burk patterns. Possible explanations for these patterns are discussed. The inhibitor is active against all catalases tested from a wide variety of organisms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHANCE B., HERBERT D. The enzymesubstrate compounds of bacterial catalase and peroxides. Biochem J. 1950 Apr;46(4):402–414. doi: 10.1042/bj0460402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HARGREAVES A. B., DEUTSCH H. F. The in vitro inhibition of catalase by a tumor factor. Cancer Res. 1952 Oct;12(10):720–726. [PubMed] [Google Scholar]
  3. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  4. Ho D. T., Scandalios J. G. Regulation of Alcohol Dehydrogenases in Maize Scutellum during Germination. Plant Physiol. 1975 Jul;56(1):56–59. doi: 10.1104/pp.56.1.56. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jolly S. O., Tolbert N. E. NADH-Nitrate Reductase Inhibitor from Soybean Leaves. Plant Physiol. 1978 Aug;62(2):197–203. doi: 10.1104/pp.62.2.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KAMPSCHMIDT R. F. MECHANISM OF LIVER CATALASE DEPRESSION IN TUMOR-BEARING ANIMALS: A REVIEW. Cancer Res. 1965 Jan;25:34–45. [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Pressey R. Invertase inhibitors from red beet, sugar beet, and sweet potato roots. Plant Physiol. 1968 Sep;43(9):1430–1434. doi: 10.1104/pp.43.9.1430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Quail P. H., Scandalios J. G. Turnover of genetically defined catalase isozymes in maize. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1402–1406. doi: 10.1073/pnas.68.7.1402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rupprecht M., Schleifer K. H. Comparative immunological study of catalases in the genus Micrococcus. Arch Microbiol. 1977 Jul 26;114(1):61–66. doi: 10.1007/BF00429631. [DOI] [PubMed] [Google Scholar]
  11. Scandalios J. G., Liu E. H., Campeau M. A. The effects of intragenic and intergenic complementation on catalase structure and function in maize: a molecular approach to heterosis. Arch Biochem Biophys. 1972 Dec;153(2):695–705. doi: 10.1016/0003-9861(72)90388-8. [DOI] [PubMed] [Google Scholar]
  12. Shainkin R., Birk Y. Alpha-amylase inhibitors from wheat. Isolation and characterization. Biochim Biophys Acta. 1970 Dec 22;221(3):502–513. doi: 10.1016/0005-2795(70)90221-7. [DOI] [PubMed] [Google Scholar]
  13. Sorenson J. C., Ganapathy P. S., Scandalios J. G. Regulation of maize catalase by changing rates of synthesis and degradation. Biochem J. 1977 Apr 15;164(1):113–117. doi: 10.1042/bj1640113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sorenson J. C., Scandalios J. G. Developmental expression of a catalase inhibitor in maize. Plant Physiol. 1976 Mar;57(3):351–352. doi: 10.1104/pp.57.3.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sorenson J. C., Scandalios J. G. Purification of a catalase inhibitor from maize by affinity chromatography. Biochem Biophys Res Commun. 1975 Mar 3;63(1):239–246. doi: 10.1016/s0006-291x(75)80035-0. [DOI] [PubMed] [Google Scholar]
  16. Tsaftaris A. S., Sorenson J. C., Scandalios J. G. Glycosylation of catalase inhibitor necessary for activity. Biochem Biophys Res Commun. 1980 Feb 12;92(3):889–895. doi: 10.1016/0006-291x(80)90786-x. [DOI] [PubMed] [Google Scholar]
  17. Wallace W. Purification and properties of a nitrate reductase-inactivating enzyme. Biochim Biophys Acta. 1974 Mar 21;341(1):265–276. doi: 10.1016/0005-2744(74)90087-4. [DOI] [PubMed] [Google Scholar]

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