Abstract
Lolium multiflorum plants were grown under a low- or high-light regime until third leaves had emerged to one-third their final length and then were transferred to a contrasting light regime. At this stage, the leaf possesses a tissue-age gradient from tip to base; thus, the reversibility of light-acclimated responses as a function of the degree of differentiation was analyzed in individual leaves.
Regional responses in the apical, medial, and basal zones of leaves of transferred plants were analyzed before and after light transfer and compared to leaves from plants kept in constant light. Leaves transferred from low to high light showed rapid recovery and attained light-saturated rates and ribulose bisphosphate carboxylase activities equivalent to those of high-light controls. However, fresh or dry weight and chlorophyll content were intermediate between those for the two irradiances. In the reciprocal experiment, the apical leaf zone retained high-light characteristics for maximum photosynthesis, whereas all the other functional parameters adapted rapidly to values characteristic of their low-light counterparts (equivalent foliar zones).
On the ultrastructural level, chloroplasts in the apical zone of transferred leaves surpassed their respective constant light controls in absolute membrane content and size. However, a shift to high light induced an increase in plastid volume and, in relative terms, the plastid membrane content was diluted. A shift to low-light treatment led to smaller membrane-dense plastids. The ultrastructural readaptation is realized through differential rates of increase in plastid volume and lamellar content. Proplastids (leaf base), or juvenile plastids having reached an intermediate developmental stage (leaf middle zone) at the time of transfer, took on characteristics of the latter light regime (equivalent to controls).
These results provide evidence for rapid reacclimation processes under changing light regimes and suggest a capacity for regional light responses along the leaf.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bunce J. A., Patterson D. T., Peet M. M. Light acclimation during and after leaf expansion in soybean. Plant Physiol. 1977 Aug;60(2):255–258. doi: 10.1104/pp.60.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgenthaler J. J., Mendiola-Morgenthaler L. Synthesis of soluble, thylakoid, and envelope membrane proteins by spinach chloroplasts purified from gradients. Arch Biochem Biophys. 1976 Jan;172(1):51–58. doi: 10.1016/0003-9861(76)90046-1. [DOI] [PubMed] [Google Scholar]
- Paolillo D. J., Jr The three-dimensional arrangement of intergranal lamellae in chloroplasts. J Cell Sci. 1970 Jan;6(1):243–255. doi: 10.1242/jcs.6.1.243. [DOI] [PubMed] [Google Scholar]