Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1980 Nov;66(5):830–834. doi: 10.1104/pp.66.5.830

The Site of the Inhibition of the Shikimate Pathway by Glyphosate

II. INTERFERENCE OF GLYPHOSATE WITH CHORISMATE FORMATION IN VIVO AND IN VITRO1

Nikolaus Amrhein 1,2, Brigitte Deus 1, Peter Gehrke 1, Hans Christian Steinrücken 1
PMCID: PMC440735  PMID: 16661535

Abstract

In the presence of the nonselective herbicide glyphosate (N-[phosphonomethyl]glycine), buckwheat (Fagopyrum esculentum Moench) hypocotyls and cultured cells of Galium mollugo L. accumulate an organic acid, which was identified as shikimate by mass-spectroscopy of its methyl ester. After growth in 0.5 millimolar glyphosate for 10 days, G. mollugo cells contained shikimate in amounts of up to 10% of their dry weight. Synthesis of chorismate-derived anthraquinones in G. mollugo was blocked by glyphosate. Chorismate and o-succinylbenzoate (an anthraquinone precursor) alleviated the inhibition. The conclusion drawn from these experiments, that glyphosate inhibits a step in the biosynthetic sequence from shikimate to chorismate, was substantiated by the finding that glyphosate is a powerful inhibitor of the conversion of shikimate to chorismate in cell-free extracts from Aerobacter aerogenes 62-1.

Full text

PDF
830

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dansette P., Azerad R. A new intermediate in naphthoquinone and menaquinone biosynthesis. Biochem Biophys Res Commun. 1970 Sep 10;40(5):1090–1095. doi: 10.1016/0006-291x(70)90906-x. [DOI] [PubMed] [Google Scholar]
  2. GAITONDE M. K., GORDON M. W. A microchemical method for the detection and determination of shikimic acid. J Biol Chem. 1958 Feb;230(2):1043–1050. [PubMed] [Google Scholar]
  3. Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
  4. Haderlie L. C., Widholm J. M., Slife F. W. Effect of glyphosate on carrot and tobacco cells. Plant Physiol. 1977 Jul;60(1):40–43. doi: 10.1104/pp.60.1.40. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Holländer H., Amrhein N. The Site of the Inhibition of the Shikimate Pathway by Glyphosate: I. INHIBITION BY GLYPHOSATE OF PHENYLPROPANOID SYNTHESIS IN BUCKWHEAT (FAGOPYRUM ESCULENTUM MOENCH) . Plant Physiol. 1980 Nov;66(5):823–829. doi: 10.1104/pp.66.5.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Holländer H., Kiltz H. H., Amrhein N. Interference of L-alpha-aminooxy-beta-phenylpropionic acid with phenylalanine metabolism in buckwheat. Z Naturforsch C. 1979 Dec;34(12):1162–1173. doi: 10.1515/znc-1979-1214. [DOI] [PubMed] [Google Scholar]
  7. Roisch U., Lingens F. Effect of the herbicide N-phosphonomethylglycine on the biosynthesis of aromatic amino acids. Angew Chem Int Ed Engl. 1974 Jun;13(6):400–400. doi: 10.1002/anie.197404001. [DOI] [PubMed] [Google Scholar]
  8. YOSHIDA S., HASEGAWA M. A micro-colorimetric method for the determination of shikimic acid. Arch Biochem Biophys. 1957 Aug;70(2):377–381. doi: 10.1016/0003-9861(57)90124-8. [DOI] [PubMed] [Google Scholar]
  9. Zenk M. H., el-Shagi H., Schulte U. Anthraquinone production by cell suspension cultures of Morinda citrifolia. Planta Med. 1975;Suppl:79–101. doi: 10.1055/s-0028-1104768. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES