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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1994 Jun 21;91(13):5748–5755. doi: 10.1073/pnas.91.13.5748

A role for molecular genetics in biological conservation.

S J O'Brien 1
PMCID: PMC44074  PMID: 7912434

Abstract

The recognition of recent accelerated depletion of species as a consequence of human industrial development has spawned a wide interest in identifying threats to endangered species. In addition to ecological and demographic perils, it has become clear that small populations that narrowly survive demographic contraction may undergo close inbreeding, genetic drift, and loss of overall genomic variation due to allelic loss or reduction to homozygosity. I review here the consequences of such genetic depletion revealed by applying molecular population genetic analysis to four endangered mammals: African cheetah, lion, Florida panther, and humpback whale. The accumulated genetic results, combined with physiological, ecological, and ethological data, provide a multifaceted perspective of the process of species diminution. An emerging role of population genetics, phylogenetics, and phylogeography as indicators of a population's natural history and its future prognosis provides valuable data of use in the development of conservation management plans for endangered species.

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Selected References

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  1. Avise J. C., Nelson W. S. Molecular genetic relationships of the extinct dusky seaside sparrow. Science. 1989 Feb 3;243(4891):646–648. doi: 10.1126/science.243.4891.646. [DOI] [PubMed] [Google Scholar]
  2. Baker C. S., Gilbert D. A., Weinrich M. T., Lambertsen R., Calambokidis J., McArdle B., Chambers G. K., O'Brien S. J. Population characteristics of DNA fingerprints in humpback whales (Megaptera novaeangliae). J Hered. 1993 Jul-Aug;84(4):281–290. doi: 10.1093/oxfordjournals.jhered.a111340. [DOI] [PubMed] [Google Scholar]
  3. Baker C. S., Palumbi S. R., Lambertsen R. H., Weinrich M. T., Calambokidis J., O'Brien S. J. Influence of seasonal migration on geographic distribution of mitochondrial DNA haplotypes in humpback whales. Nature. 1990 Mar 15;344(6263):238–240. doi: 10.1038/344238a0. [DOI] [PubMed] [Google Scholar]
  4. Baker C. S., Perry A., Bannister J. L., Weinrich M. T., Abernethy R. B., Calambokidis J., Lien J., Lambertsen R. H., Ramírez J. U., Vasquez O. Abundant mitochondrial DNA variation and world-wide population structure in humpback whales. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8239–8243. doi: 10.1073/pnas.90.17.8239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Black F. L. Why did they die? Science. 1992 Dec 11;258(5089):1739–1740. doi: 10.1126/science.1465610. [DOI] [PubMed] [Google Scholar]
  6. Bonnel M. L., Selander R. K. Elephant seals: genetic variation and near extinction. Science. 1974 May 24;184(4139):908–909. doi: 10.1126/science.184.4139.908. [DOI] [PubMed] [Google Scholar]
  7. Cann R. L., Stoneking M., Wilson A. C. Mitochondrial DNA and human evolution. Nature. 1987 Jan 1;325(6099):31–36. doi: 10.1038/325031a0. [DOI] [PubMed] [Google Scholar]
  8. Geist V. Endangered species and the law. Nature. 1992 May 28;357(6376):274–276. doi: 10.1038/357274a0. [DOI] [PubMed] [Google Scholar]
  9. Gibbons A. Mission impossible: saving all endangered species. Science. 1992 Jun 5;256(5062):1386–1386. doi: 10.1126/science.256.5062.1386. [DOI] [PubMed] [Google Scholar]
  10. Gilbert D. A., Packer C., Pusey A. E., Stephens J. C., O'Brien S. J. Analytical DNA fingerprinting in lions: parentage, genetic diversity, and kinship. J Hered. 1991 Sep-Oct;82(5):378–386. doi: 10.1093/oxfordjournals.jhered.a111107. [DOI] [PubMed] [Google Scholar]
  11. Heeney J. L., Evermann J. F., McKeirnan A. J., Marker-Kraus L., Roelke M. E., Bush M., Wildt D. E., Meltzer D. G., Colly L., Lukas J. Prevalence and implications of feline coronavirus infections of captive and free-ranging cheetahs (Acinonyx jubatus). J Virol. 1990 May;64(5):1964–1972. doi: 10.1128/jvi.64.5.1964-1972.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hoelzel A. R., Halley J., O'Brien S. J., Campagna C., Arnbom T., Le Boeuf B., Ralls K., Dover G. A. Elephant seal genetic variation and the use of simulation models to investigate historical population bottlenecks. J Hered. 1993 Nov-Dec;84(6):443–449. doi: 10.1093/oxfordjournals.jhered.a111370. [DOI] [PubMed] [Google Scholar]
  13. Lande R. Genetics and demography in biological conservation. Science. 1988 Sep 16;241(4872):1455–1460. doi: 10.1126/science.3420403. [DOI] [PubMed] [Google Scholar]
  14. Lewin R. Limits to DNA fingerprinting. Science. 1989 Mar 24;243(4898):1549–1551. doi: 10.1126/science.2928790. [DOI] [PubMed] [Google Scholar]
  15. Lewontin R. C., Hubby J. L. A molecular approach to the study of genic heterozygosity in natural populations. II. Amount of variation and degree of heterozygosity in natural populations of Drosophila pseudoobscura. Genetics. 1966 Aug;54(2):595–609. doi: 10.1093/genetics/54.2.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lynch M. Estimation of relatedness by DNA fingerprinting. Mol Biol Evol. 1988 Sep;5(5):584–599. doi: 10.1093/oxfordjournals.molbev.a040518. [DOI] [PubMed] [Google Scholar]
  17. Marshall L. G., Webb S. D., Sepkoski J. J., Jr, Raup D. M. Mammalian evolution and the great american interchange. Science. 1982 Mar 12;215(4538):1351–1357. doi: 10.1126/science.215.4538.1351. [DOI] [PubMed] [Google Scholar]
  18. Menotti-Raymond M., O'Brien S. J. Dating the genetic bottleneck of the African cheetah. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3172–3176. doi: 10.1073/pnas.90.8.3172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nei M., Chakraborty R. Genetic distance and electrophoretic identity of proteins between taxa. J Mol Evol. 1973 Nov 27;2(4):323–328. doi: 10.1007/BF01654100. [DOI] [PubMed] [Google Scholar]
  20. O'Brien S. J., Roelke M. E., Marker L., Newman A., Winkler C. A., Meltzer D., Colly L., Evermann J. F., Bush M., Wildt D. E. Genetic basis for species vulnerability in the cheetah. Science. 1985 Mar 22;227(4693):1428–1434. doi: 10.1126/science.2983425. [DOI] [PubMed] [Google Scholar]
  21. O'brien S. J., Mayr E. Bureaucratic mischief: recognizing endangered species and subspecies. Science. 1991 Mar 8;251(4998):1187–1188. doi: 10.1126/science.251.4998.1187. [DOI] [PubMed] [Google Scholar]
  22. O'brien S. J., Mayr E. Bureaucratic mischief: recognizing endangered species and subspecies. Science. 1991 Mar 8;251(4998):1187–1188. doi: 10.1126/science.251.4998.1187. [DOI] [PubMed] [Google Scholar]
  23. O'brien S. J., Wildt D. E., Goldman D., Merril C. R., Bush M. The cheetah is depauperate in genetic variation. Science. 1983 Jul 29;221(4609):459–462. doi: 10.1126/science.221.4609.459. [DOI] [PubMed] [Google Scholar]
  24. Olmsted R. A., Langley R., Roelke M. E., Goeken R. M., Adger-Johnson D., Goff J. P., Albert J. P., Packer C., Laurenson M. K., Caro T. M. Worldwide prevalence of lentivirus infection in wild feline species: epidemiologic and phylogenetic aspects. J Virol. 1992 Oct;66(10):6008–6018. doi: 10.1128/jvi.66.10.6008-6018.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pimm S. L., Gittleman J. L. Biological diversity: where is it? Science. 1992 Feb 21;255(5047):940–940. doi: 10.1126/science.1546290. [DOI] [PubMed] [Google Scholar]
  26. Ralls K., Brugger K., Ballou J. Inbreeding and juvenile mortality in small populations of ungulates. Science. 1979 Nov 30;206(4422):1101–1103. doi: 10.1126/science.493997. [DOI] [PubMed] [Google Scholar]
  27. Roelke M. E., Martenson J. S., O'Brien S. J. The consequences of demographic reduction and genetic depletion in the endangered Florida panther. Curr Biol. 1993 Jun 1;3(6):340–350. doi: 10.1016/0960-9822(93)90197-v. [DOI] [PubMed] [Google Scholar]
  28. Schrier P. I., Bernards R., Vaessen R. T., Houweling A., van der Eb A. J. Expression of class I major histocompatibility antigens switched off by highly oncogenic adenovirus 12 in transformed rat cells. 1983 Oct 27-Nov 2Nature. 305(5937):771–775. doi: 10.1038/305771a0. [DOI] [PubMed] [Google Scholar]
  29. Sørensen T. I., Nielsen G. G., Andersen P. K., Teasdale T. W. Genetic and environmental influences on premature death in adult adoptees. N Engl J Med. 1988 Mar 24;318(12):727–732. doi: 10.1056/NEJM198803243181202. [DOI] [PubMed] [Google Scholar]
  30. Vigilant L., Pennington R., Harpending H., Kocher T. D., Wilson A. C. Mitochondrial DNA sequences in single hairs from a southern African population. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9350–9354. doi: 10.1073/pnas.86.23.9350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wildt D. E., Bush M., Howard J. G., O'Brien S. J., Meltzer D., Van Dyk A., Ebedes H., Brand D. J. Unique seminal quality in the South African cheetah and a comparative evaluation in the domestic cat. Biol Reprod. 1983 Nov;29(4):1019–1025. doi: 10.1095/biolreprod29.4.1019. [DOI] [PubMed] [Google Scholar]
  32. Wildt D. E., O'Brien S. J., Howard J. G., Caro T. M., Roelke M. E., Brown J. L., Bush M. Similarity in ejaculate-endocrine characteristics in captive versus free-ranging cheetahs of two subspecies. Biol Reprod. 1987 Mar;36(2):351–360. doi: 10.1095/biolreprod36.2.351. [DOI] [PubMed] [Google Scholar]
  33. Yuhki N., O'Brien S. J. DNA variation of the mammalian major histocompatibility complex reflects genomic diversity and population history. Proc Natl Acad Sci U S A. 1990 Jan;87(2):836–840. doi: 10.1073/pnas.87.2.836. [DOI] [PMC free article] [PubMed] [Google Scholar]

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