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. 1994 Jun 21;91(13):5761–5765. doi: 10.1073/pnas.91.13.5761

Arabidopsis floral homeotic gene BELL (BEL1) controls ovule development through negative regulation of AGAMOUS gene (AG).

A Ray 1, K Robinson-Beers 1, S Ray 1, S C Baker 1, J D Lang 1, D Preuss 1, S B Milligan 1, C S Gasser 1
PMCID: PMC44076  PMID: 7912435

Abstract

Ovules are the developmental precursors of seeds. In angiosperms the ovules are enclosed within the central floral organs, the carpels. We have identified a homeotic mutation in Arabidopsis, "bell" (bel1), which causes transformation of ovule integuments into carpels. In situ hybridization analysis shows that this mutation leads to increased expression of the carpel-determining homeotic gene AGAMOUS (AG) in the mutant ovules. Introduction of a constitutively expressed AG transgene into wild-type plants causes the ovules to resemble those of bel1 mutants. We propose that the BEL1 gene product directs normal integument development, in part by suppressing AG expression in this structure. Our results allow expansion of the current model of floral organ identity to include regulation of ovule integument identity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowman J. L., Drews G. N., Meyerowitz E. M. Expression of the Arabidopsis floral homeotic gene AGAMOUS is restricted to specific cell types late in flower development. Plant Cell. 1991 Aug;3(8):749–758. doi: 10.1105/tpc.3.8.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bowman J. L., Smyth D. R., Meyerowitz E. M. Genetic interactions among floral homeotic genes of Arabidopsis. Development. 1991 May;112(1):1–20. doi: 10.1242/dev.112.1.1. [DOI] [PubMed] [Google Scholar]
  3. Carpenter R., Coen E. S. Floral homeotic mutations produced by transposon-mutagenesis in Antirrhinum majus. Genes Dev. 1990 Sep;4(9):1483–1493. doi: 10.1101/gad.4.9.1483. [DOI] [PubMed] [Google Scholar]
  4. Coen E. S., Meyerowitz E. M. The war of the whorls: genetic interactions controlling flower development. Nature. 1991 Sep 5;353(6339):31–37. doi: 10.1038/353031a0. [DOI] [PubMed] [Google Scholar]
  5. Drews G. N., Bowman J. L., Meyerowitz E. M. Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product. Cell. 1991 Jun 14;65(6):991–1002. doi: 10.1016/0092-8674(91)90551-9. [DOI] [PubMed] [Google Scholar]
  6. Evans P. T., Malmberg R. L. Alternative pathways of tobacco placental development: time of commitment and analysis of a mutant. Dev Biol. 1989 Nov;136(1):273–283. doi: 10.1016/0012-1606(89)90148-6. [DOI] [PubMed] [Google Scholar]
  7. Gasser C. S., Robinson-Beers K. Pistil Development. Plant Cell. 1993 Oct;5(10):1231–1239. doi: 10.1105/tpc.5.10.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gustafson-Brown C., Savidge B., Yanofsky M. F. Regulation of the arabidopsis floral homeotic gene APETALA1. Cell. 1994 Jan 14;76(1):131–143. doi: 10.1016/0092-8674(94)90178-3. [DOI] [PubMed] [Google Scholar]
  9. Hagberg L., Hull R., Hull S., Falkow S., Freter R., Svanborg Edén C. Contribution of adhesion to bacterial persistence in the mouse urinary tract. Infect Immun. 1983 Apr;40(1):265–272. doi: 10.1128/iai.40.1.265-272.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jack T., Brockman L. L., Meyerowitz E. M. The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens. Cell. 1992 Feb 21;68(4):683–697. doi: 10.1016/0092-8674(92)90144-2. [DOI] [PubMed] [Google Scholar]
  11. Kunst L., Klenz J. E., Martinez-Zapater J., Haughn G. W. AP2 Gene Determines the Identity of Perianth Organs in Flowers of Arabidopsis thaliana. Plant Cell. 1989 Dec;1(12):1195–1208. doi: 10.1105/tpc.1.12.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lander E. S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S. E., Newberg L. A., Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. doi: 10.1016/0888-7543(87)90010-3. [DOI] [PubMed] [Google Scholar]
  13. Mandel M. A., Bowman J. L., Kempin S. A., Ma H., Meyerowitz E. M., Yanofsky M. F. Manipulation of flower structure in transgenic tobacco. Cell. 1992 Oct 2;71(1):133–143. doi: 10.1016/0092-8674(92)90272-e. [DOI] [PubMed] [Google Scholar]
  14. Meyerowitz E. M., Bowman J. L., Brockman L. L., Drews G. N., Jack T., Sieburth L. E., Weigel D. A genetic and molecular model for flower development in Arabidopsis thaliana. Dev Suppl. 1991;1:157–167. [PubMed] [Google Scholar]
  15. Mizukami Y., Ma H. Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity. Cell. 1992 Oct 2;71(1):119–131. doi: 10.1016/0092-8674(92)90271-d. [DOI] [PubMed] [Google Scholar]
  16. Modrusan Z., Reiser L., Feldmann K. A., Fischer R. L., Haughn G. W. Homeotic Transformation of Ovules into Carpel-like Structures in Arabidopsis. Plant Cell. 1994 Mar;6(3):333–349. doi: 10.1105/tpc.6.3.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robinson-Beers K., Pruitt R. E., Gasser C. S. Ovule Development in Wild-Type Arabidopsis and Two Female-Sterile Mutants. Plant Cell. 1992 Oct;4(10):1237–1249. doi: 10.1105/tpc.4.10.1237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schwarz-Sommer Z., Huijser P., Nacken W., Saedler H., Sommer H. Genetic Control of Flower Development by Homeotic Genes in Antirrhinum majus. Science. 1990 Nov 16;250(4983):931–936. doi: 10.1126/science.250.4983.931. [DOI] [PubMed] [Google Scholar]
  19. Sommer H., Beltrán J. P., Huijser P., Pape H., Lönnig W. E., Saedler H., Schwarz-Sommer Z. Deficiens, a homeotic gene involved in the control of flower morphogenesis in Antirrhinum majus: the protein shows homology to transcription factors. EMBO J. 1990 Mar;9(3):605–613. doi: 10.1002/j.1460-2075.1990.tb08152.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tröbner W., Ramirez L., Motte P., Hue I., Huijser P., Lönnig W. E., Saedler H., Sommer H., Schwarz-Sommer Z. GLOBOSA: a homeotic gene which interacts with DEFICIENS in the control of Antirrhinum floral organogenesis. EMBO J. 1992 Dec;11(13):4693–4704. doi: 10.1002/j.1460-2075.1992.tb05574.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Valvekens D., Van Montagu M., Van Lijsebettens M. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536–5540. doi: 10.1073/pnas.85.15.5536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yanofsky M. F., Ma H., Bowman J. L., Drews G. N., Feldmann K. A., Meyerowitz E. M. The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors. Nature. 1990 Jul 5;346(6279):35–39. doi: 10.1038/346035a0. [DOI] [PubMed] [Google Scholar]

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