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. 1980 Dec;66(6):1037–1043. doi: 10.1104/pp.66.6.1037

Electrophoretic Comparison of Polypeptides from Enriched Plasma Membrane Fractions from Developing Soybean Roots 1

Martha L Booz 1, Robert L Travis 1
PMCID: PMC440785  PMID: 16661572

Abstract

The polypeptide complement of enriched soybean (Glycine max [L.] Merr. cult. wells) root plasma membrane fractions was studied by two-dimensional gel electrophoresis. Good resolution was obtained when polypeptides were solubilized in sodium dodecyl sulfate and when butylated hydroxytoluene was included in the vesicle isolation and solubilization media. The pattern obtained on the two-dimensional slab gel for root plasma membrane was characteristic for that membrane. The polypeptide complements from mitochondrial membranes and from enriched fractions of three other endomembrane components were solubilized and electrophoresed for comparison. Each membrane preparation was identifiable on the basis of its characteristic electrophoretogram. Electrophoresis of protein solubilized from plasma membrane fractions isolated from meristematic and mature root tissue revealed both qualitative and quantitative differences in the respective protein complements.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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