Table 4.
Embryonic phenotypes resulting from Rp-dl pRNAi
| dsRNA (μg/μl) | Embryonic phenotypes (%) | n | |||
|---|---|---|---|---|---|
| No germ rudiment* | Embryo anteriorly localized** | Embryo randomly localized | Embryo posteriorly localized | ||
| Rp-dl (0.5) stages 2-4 | 44 | 50 | 5 | 0 | 18 |
| Rp-dl (1.0) stages 2-4 | 32 | 52 | 4 | 12 | 25 |
| Rp-dl (0.5) stages 5-10 | 22 | 68 | 7 | 3 | 59 |
| Rp-Toll (0.5) stages 2-10 | 7 | 23 | 30 | 40 | 30 |
| control (1.0) stages 2-10 | 6 | - | - | 94 | 50 |
*“No germ rudiment” phenotype includes empty eggs and halted embryos that do not form a germ rudiment. **At stage 2 embryos anteriorly localized display embryonic rudiment perpendicular to long axis of egg; after stage 5 anteriorly localized embryos are short, with absent or small appendage-like structures (25% penetrant appendage-like phenotype).