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. 2014 Dec 26;6(5):3195–3210. doi: 10.18632/oncotarget.3084

Figure 6. Effect of siAR, siMNK1 on LNCaP cell viability, and VNLG-152 on key and downstream proteins in LNCaP cells.

Figure 6

(A) Western blot analysis of the expression of fAR, MNK1 and peIF4E in LNCaP cells transfected with 100 nM of siAR, siMNK1 and combinations for 18 h. (B) Effect of VNLG-152 (5 μmol) on cell proliferation in LNCaP cells transfected with siAR, siMNK1 and combinations as determined by MTT assay. The results represent the mean ± SEM of three independent experiments and are represented as a bar graph after normalizing to control cells. *, P< 0.05; ♣, P<0.01 compared with vehicle treated control. (C) Dose-dependent effect of VNLG-152 on the expression of AR, MNKs, peIF4E and downstream target proteins. Total cell lysate from LNCaP cells treated with VNLG-152 (0 - 20 μmol/L) for 24 h were separated by SDS-PAGE and probed with corresponding antibodies. Vehicle treated cells were included as control and all blots were reprobed for β-actin to ensure equal protein loading.