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. 2014 Dec 31;6(5):3359–3374. doi: 10.18632/oncotarget.3245

Figure 4. Anti-proliferative and pro-apoptotic effects of Aurora B inhibition in Group 3 medulloblastoma cells.

Figure 4

A) MTS cell viability assay of D425 and D458 cells exposed to varying concentration of AZD1152-HQPA over a 96 hour period. Error bars represent standard error of the mean of 8 replicates per group per time point. B) Western blots showing the inhibition of Histone H3 (Ser 10) phosphorylation and Aurora B autophosphorylation by AZD1152-HQPA at 100 nM in D425 and D458 endogenous MYC overexpressing cells. Labels as follows: phosphohistone H3 Serine 10 (pH3 Ser 10), histone H3 (H3), aurora kinase A (AurA), aurora kinase B (AurB), Aurora A phosphothreonine 288 (pAurA), Aurora B phosphothreonine 232 (pAurB). Total protein loaded 30 μg. Note the lower band observed in the AurA blot is non-specific binding after re-probing of the membrane. C) Western blot for MYC in D425 and D458 cells exposed to 0.01% DMSO or 100 nM AZD1152-HQPA for 48 hours. Total protein loaded 30 μg. D) Western blots for cleaved caspase-3 (CC3) in D425 and D458 cells exposed to 0.01% DMSO or 100 nM AZD1152-HQPA for 48 hours. Positive control (+ CON) was 10 μL of etoposide treated Jurkat cell lysate (Cell Signaling).