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Therapeutic Advances in Gastroenterology logoLink to Therapeutic Advances in Gastroenterology
. 2015 May;8(3):136–142. doi: 10.1177/1756283X15572580

The clinical potential of ramosetron in the treatment of irritable bowel syndrome with diarrhea (IBS-D)

Yang Won Min 1, Poong-Lyul Rhee 2,
PMCID: PMC4416292  PMID: 25949526

Abstract

Irritable bowel syndrome (IBS) is a highly prevalent functional bowel disorder. Serotonin (5-HT) is known to play a physiological and pathophysiological role in the regulation of gastrointestinal function. In experimental studies, 5-HT3 receptor antagonists have been reported to slow colon transit, to blunt gastrocolonic reflex, and to reduce rectal sensitivity. Alosetron and cilansetron, potent and selective 5-HT3 receptor antagonists, have proven efficacy in the treatment of IBS with diarrhea (IBS-D). However, alosetron was voluntarily withdrawn due to postmarketing reports of ischemic colitis and complications of constipation, and cilansetron was never marketed. Currently alosetron is available under a risk management program for women with severe IBS-D. Ramosetron is another potent and selective 5-HT3 receptor antagonist, which has been marketed in Japan, South Korea, and Taiwan. In animal studies, ramosetron reduced defecation induced by corticotrophin-releasing hormone and had inhibitory effects on colonic nociception. In two randomized controlled studies including 957 patients with IBS-D, ramosetron increased monthly responder rates of patient-reported global assessment of IBS symptom relief compared with placebo. Ramosetron was also as effective as mebeverine in male patients with IBS-D. In a recent randomized controlled trial with 343 male patients with IBS-D, ramosetron has proved effective in improving stool consistency, relieving abdominal pain/discomfort, and improving health-related quality of life. Regarding safety, ramosetron is associated with a lower incidence of constipation compared with other 5-HT3 receptor antagonists and has not been associated with ischemic colitis. Although further large prospective studies are needed to assess whether ramosetron is effective for female patients with IBS-D and to evaluate its long-term safety, ramosetron appears to be one of the most promising agents for patients with IBS-D.

Keywords: diarrhea, irritable bowel syndrome, ramosetron, serotonin, 5-HT3

Introduction

Irritable bowel syndrome (IBS) is a functional bowel disorder with recurrent abdominal pain and/or discomfort and disordered defecation [Longstreth et al. 2006]. Although the prevalence of IBS varies among countries, global pooled prevalence in 80 separate adult studies is 11.2% [95% confidence interval (CI) 9.8–12.8%] [Lovell and Ford, 2012]. According to predominant stool pattern [Longstreth et al. 2006], IBS with diarrhea (IBS-D) accounts for 23.4% (95% CI 8.4%–42.6%) of patients with IBS [Lovell and Ford, 2012].

Although the pathogenesis of symptoms in IBS is incompletely understood, altered bowel motility, visceral hypersensitivity, mucosal immune activation, increased mucosal permeability, enteric neuromuscular dysfunction, abnormal brain–gut interactions, alteration in the gut microbiome, and psychological disturbance have been hypothesized [Camilleri, 2005, 2012; Hasler, 2011]. Serotonin type 3 (5-HT3) receptor antagonists have been reported to slow colon transit, to blunt gastrocolonic reflex, and to reduce rectal sensitivity and postprandial motility [Talley et al. 1990; Prior and Read, 1993; Bjornsson et al. 1998]. Thus more potent and specific 5-HT3 receptor antagonists (alosetron and cilansetron) were developed and their efficacy has been well established in IBS-D [Andresen et al. 2008]. However, alosetron was voluntarily withdrawn due to postmarketing reports of ischemic colitis and complications of constipation, and cilansetron was never marketed. Currently alosetron is available under a risk management program for women with severe IBS-D [Tong et al. 2013].

Ramosetron, another potent and selective 5-HT3 receptor antagonist [Hirata et al. 2007], has been marketed in Japan since 1996 and has been used as an antiemetic for patients with cancer [Rabasseda, 2002]. In animal studies, ramosetron showed control effects on stress-induced defecation disturbance and inhibitory effect on colonic nociception [Miyata et al. 1998; Funatsu et al. 2007; Hirata et al. 2008]. From these observations ramosetron has also been used as a new agent for IBS-D. This article will review the efficacy and safety of ramosetron in IBS-D and will address its clinical potential.

Mechanism of action and animal studies of ramosetron

The gut contains about 95% of the body’s 5-HT [Gershon and Tack, 2007]. 5-HT plays important physiological roles in the regulation of gastrointestinal function [Beattie and Smith, 2008]. Receptors mediating the actions of 5-HT amount to about 14 different receptors (from 5-HT1 to 5-HT7 and its subtypes) that produce diverse effects on a wide range of targets [Peroutka, 1994; Beattie and Smith, 2008]. 5-HT3 receptors are widely distributed in the central and peripheral nervous system [Farber et al. 2004]. Intraluminal stimuli stimulate the release of 5-HT from enterochromaffin cells located in the mucosa [Crowell, 2004; Smith et al. 2014]. When secreted, 5-HT can activate 5-HT3 receptors located on intrinsic primary afferent neurons with submucosal terminals, thus initiating the peristaltic reflex and modulating intestinal secretion [Gershon, 1999; Cooke, 2000]. 5-HT also stimulates 5-HT3 and 5-HT4 receptors located on primary afferent neurons of both splanchnic and vagal fibers, thereby modulating both sensory and motor responses [Crowell, 2004].

In the animal studies, ramosetron demonstrated logical results that are consistent with expected effects of 5-HT3 receptor antagonists. In rats, corticotrophin-releasing hormone (CRH) exogenously administered or released from the central nervous system by stress peripherally promoted the release of 5-HT which in turn stimulated defecation through the 5-HT3 receptor and ramosetron reduced the defecation by CRH in a dose-dependent manner [Miyata et al. 1998]. Ramosetron also inhibited defecation induced by emotional stress and retarded stress-stimulated colonic transit in rats [Funatsu et al. 2007]. Unlike existing antidiarrheal and spasmolytic agents, ramosetron, had inhibitory effects on colonic nociception in rats [Hirata et al. 2008]. Collectively, ramosetron appears to have the pharmacological action for IBS-D through targeting 5-HT3 receptor.

Efficacy of ramosetron in IBS-D

5-HT3 receptor antagonist has been well demonstrated as an effective drug class in the treatment of IBS-D [Andresen et al. 2008]. A meta-analysis in 2008 [Andresen et al. 2008] including 14 randomized controlled trials (RCTs) with 3024 patients receiving alosetron and 1116 receiving cilasetron compared with 3043 placebo or 304 mebeverine found 5-HT3 receptor antagonists more effective than the comparators in achieving global improvement in IBS symptoms [pooled relative risk (RR) 1.60; 95% CI 1.49–1.72) and relief of abdominal pain/discomfort (pooled RR 1.30; 95% CI 1.22–1.39). Benefit was apparent for both agents, in patients of either sex. An old 5-HT3 receptor antagonist widely used as an antiemetic, ondansetron, has showed benefits in patients with IBS in small trials [Steadman et al. 1992; Goldberg et al. 1996]. Recently, a large RCT of 120 patients with IBS-D showed that ondansetron of 4 mg for 5 weeks improves stool consistency, reduced urgency, frequency, and bloating, but does not reduce abdominal pain [Garsed et al. 2014].

Until now, four RCTs of ramosetron in the treatment of patients with IBS-D have been reported in the literature (Table 1) [Matsueda et al. 2008; Lee et al. 2011; Fukudo et al. 2014]. In two randomized, double-blind, placebo-controlled studies [Matsueda et al. 2008] of 957 male and female Japanese patients with IBS-D meeting Rome II criteria, ramosetron 5 µg and 10 µg once daily increased the monthly responder rate of patient-reported global assessment of relief of IBS symptoms compared with placebo (42.57–47%, 43.01%, and 26.92–27% for 5 µg, 10 µg, and placebo, respectively). Ramosetron also hardened stool form, decreased stool frequency, and increased the rate of days without bowel urgency. Although the relative potency of ramosetron is 7.5–22 times that of alosetron, the doses used in the trials, 5 μg and 10 μg, are very low compared with the standard alosetron dose of 2 mg (equivalent to ramosetron 90–270 μg) [Hirata et al. 2007]. In the Korean multicenter, randomized, open-label, noninferiority comparative study [Lee et al. 2011] of 343 male patients with IBS-D meeting Rome III criteria, ramosetron 5 µg once daily increased the responder rates for global IBS symptoms, abdominal pain/discomfort, and abnormal bowel habits during the treatment period, which was as effective as mebeverine 135 mg three times daily. Severity of abdominal pain/discomfort, Bristol stool form scale score, and stool frequency were also improved in both groups compared with baseline. However, this study is limited because the exact efficacy of ramosetron over placebo was not evaluated. Furthermore, mebeverine, the control drug in this study, showed no statistically significant efficacy in global improvement of IBS [Darvish-Damavandi et al. 2010]. Recently, a multicenter, randomized, double-blinded, placebo-controlled clinical study of 343 male Japanese patients with IBS-D meeting Rome III criteria has been reported [Fukudo et al. 2014]. Ramosetron 5 µg once daily improved stool consistency in the first months (the primary end point) more than placebo (50.3% versus 19.6%, p < 0.001), and the number needed to treat was 3.25 (95% CI 2.44–4.89). In addition, monthly responder rates of global assessment of relief of overall IBS symptoms and relief of abdominal pain/discomfort were higher in the ramosetron group than in the placebo group at all evaluation points. Bristol stool form scale scores were lower and changes in stool frequencies from baseline per week were higher in the ramosetron group than in the placebo group. The percentages of days without urgency increased and of days without incomplete evacuation were significantly increased in the ramosetron group compared with placebo. Significantly greater improvements in overall health-related quality of life scores were observed in the ramosetron group than in the placebo group. In these trials, ramosetron has proved its efficacy in relieving symptoms in IBS-D.

Table 1.

Clinical studies of ramosetron in irritable bowel syndrome with diarrhea.

Study Design N Sex Agents and dosing Treatment duration Primary end point and efficacy Adverse events
Fukudo et al. [2014] Randomized, double-blinded, placebo-controlled trial 296 Male Ramosetron 5 µg or placebo 12 weeks Monthly responder rates of improvement in stool consistency in the first monthRamosetron versus placebo50.3% versus 19.6% (p < 0.001) Ramosetron versus placebo
- Hard stool (8.2% versus 1.3%, p = 0.01)
- Constipation (3.4% versus 0.7%, p = 0.12)
No ischemic colitis or severe constipation
Lee et al. [2011] Randomized, open-label, parallel-group, non-inferiority comparative study 343 Male Ramosetron 5 µg or mebeverine 135 mg three times daily 4 weeks Weekly responder rate of patient-reported assessment of relief of IBS symptomsRamosetron versus mebeverine
37% versus 38%
Ramosetron versus mebeverine
- Constipation (2% versus 1%, p = 0.372)
No ischemic colitis or severe constipation
Matsueda et al. [2008] Randomized, double-blind, placebo-controlled trial 539 Mixed Ramosetron 5 µg or placebo 12 weeks Monthly responder rate of ‘Patient-reported global assessment of relief of IBS symptoms’Ramosetron versus placebo47% versus 27% (p < 0.001) Ramosetron versus placebo
- Hard stool (7.41% versus 0.74%, p < 0.001)
- Constipation (5.19% versus 1.86%, p = 0.063)
No ischemic colitis or severe constipation
Matsueda et al. [2008] Randomized, double-blind, placebo-controlled trial 418 Mixed Ramosetron, 1, 5, 10 µg, or placebo 12 weeks Monthly responder rate of ‘Patient-reported global assessment of relief of IBS symptoms’Ramosetron 5 µg versus 10 µg versus placebo42.57% versus 43.01% versus 26.92% (p < 0.05) Ramosetron 10, 5, 1 µg, and placebo
- Hard stool (8.9%, 6.8%, 1.0%, and 0%)
- Constipation (14.9%, 5.8%, 1.9%, 2.8%)
No ischemic colitis or severe constipation

Unlike the results of a phase II trial, a phase III trial demonstrated significant effects of ramosetron on relief of IBS symptoms at all evaluation points in male patients with IBS-D but not in female patients [Matsueda et al. 2008]. In female patients, it was only observed at month 2 (not at month 1 and month 3 and final point). However, these results are thought to be mainly due to the small sample size of female patients. Eighty percent of the patients enrolled in this trial were male because IBS-D is more prevalent in men [Kumano et al. 2004]. Conversely, alosetron failed to demonstrate efficacy in male patients with IBS-D [Bardhan et al. 2000] and was indicated for only female patients in the USA. Later, alosetron has shown significant efficacy in relief of IBS pain/discomfort and stool consistency in male patients with IBS-D [Chang et al. 2005]. The lack of efficacy of alosetron in male patients was likely due to the small numbers of men enrolled. However, there are several factors contributing to differences in the treatment response of male and female patients with IBS. Alosetron has different pharmacokinetics by sex. Serum concentrations of alosetron were higher in women than in men for a given dose, resulting from a sex difference in clearance by metabolism [Koch et al. 2002] and systemic exposure to alosetron was higher in women due to lower cytochrome P450 enzyme 1A2 activity than in men [Koch et al. 2004]. In fact, the impact of alosetron on intestinal transit time in patients with IBS-D is significantly greater in women than men [Viramontes et al. 2001]. In addition, alosetron and ondansetron showed lower threshold of inhibiting migrating motor complex of murine bowel in female mice than in male mice [Bush et al. 2001]. Genetic polymorphisms at the serotonin reuptake transporter (SERT) protein promoter influence response to 5-HT3 receptor antagonists in IBS-D [Camilleri et al. 2002]. Niesler and colleagues have shown that male patients with IBS-D have a reduced frequency of the 5-HTTLPR (ss) genotype compared with female patients (10% versus 19.4%) [Niesler et al. 2010]. In a recent study, the effect of ondansetron on the gut transit, however, did not differ between the SERT promoter polymorphisms [Garsed et al. 2014]. Likewise, it is not known whether differences in SERT protein polymorphisms between female and male patients contribute to the different clinical responses to 5-HT3 receptor antagonists. In addition, there are important polymorphisms in tryptophan hydroxylase 1 and 5-HT3 receptor which might be more relevant [Kilpatrick et al. 2011; Grasberger et al. 2013]. Another possible explanation for the greater efficacy of alosetron in women is due to sex-related differences in its central effects. Male and female patients with IBS differ in their brain response to a visceral stimulus and a condition [Berman et al. 2000; Naliboff et al. 2003]. Female patients show greater activation of affective and autonomic regions (ventromedial prefrontal cortex, infragenual cingulate, and amygdala). Alosetron decreases brain activity in response to rectosigmoid stimuli in structures of the emotional motor system (the amygdala, ventral striatum, hypothalamus, and infragenual cingulate gyrus), and this is associated with symptom improvement in nonconstipated patients with IBS [Mayer et al. 2002]. Thus, greater efficacy of alosetron in women with IBS may be due to its inhibitory effect on the emotional regions of the brain which are more activated in women than in men with IBS in response to visceral stimuli. However, ramosetron is known not to act centrally because P-glycoprotein at the blood–brain barrier efflux ramosetron to circulating blood [Yamamoto et al. 2002]. Although sex-related differences in pharmacologic profile have not been invested in ramosetron it might be natural to assume that ramosetron would also be effective in females like other 5-HT3 receptor antagonists. However, further studies on whether ramosetron is effective in female patients with IBS-D are necessary to draw firm conclusions.

Safety of ramosetron in IBS-D

Severe constipation and ischemic colitis are possible serious adverse events that might be from the classic effects of 5-HT3 receptor antagonist. According to a meta-analysis [Andresen et al. 2008] with 14 RCTs including 3221 patients receiving alosetron and 1116 receiving cilasetron compared with 3647 controls, 5-HT3 receptor antagonists increased the risk of constipation (pooled relative RR 4.28; 95% CI 3.28–5.60) and the number needed to harm was 4.7. The risk of constipation was higher in the studies with alosetron (RR 4.89; 95% CI 3.6–6.56) compared with the studies with cilansetron (RR 2.92; 95% CI 1.85–4.63). Approximately 10–40% of the participants who developed constipation withdrew from the trials for this reason. However, most cases of constipation in these trials were considered mild to moderate in severity and no serious events as a result of constipation were reported. In this meta-analysis, nine cases (0.2%), all from the patients receiving alosetron, in the 5-HT3 receptor antagonist treatment group had at least possible ischemic colitis and yielded an RR for ischemic colitis of 16.01 (95% CI 0.93–275; p = 0.06). A recent study [Tong et al. 2013] evaluating 29,072 patients receiving alosetron under a risk management plan during the 9-year postmarketing period reported the cumulative adjudicated incidence rate of ischemic colitis (1.03 cases/1000 patient-years) and that of complications of constipation (0.25 cases/1000 patient-years). The incidence rate of ischemic colitis has remained rare and stable, while the incidence rate of complications of constipation has sharply decreased.

According to four RCTs [Matsueda et al. 2008; Lee et al. 2011; Fukudo et al. 2014] including 696 patients receiving ramosetron (5 µg once daily) compared with 526 placebo and 168 mebeverine (135 mg three times daily), the frequency of constipation with ramosetron (2.0–5.19%) seems lower than the 27.6% and 16.2% observed in the studies with alosetron and cilansetron [Andresen et al. 2008], respectively. However, it is worth noting that ramosetron has been evaluated for mostly men, only in Asian populations, and for relatively short treatment periods. Thus, further studies with longer treatment duration and in the different populations are required to confirm if constipation is less in ramosetron than other 5-HT3 receptor antagonists. In addition, severe constipation or ischemic colitis, which has been observed with alosetron, was not reported with ramosetron. However, considering the incidence rates of ischemic colitis (1.03 cases/1000 patient-years) and complications of constipation (0.25 cases/1000 patient-years), it is a little early to conclude that ramosetron is safer than alosetron. Furthermore, ondansetron also shows a low constipation rate (9%) without a report of ischemic colitis [Garsed et al. 2014]. The pathophysiological mechanism of ischemic colitis in patients treated with 5-HT3 receptor antagonist is uncertain. In a phase II trial with ramosetron, the incidence rates of constipation and hard stool increased in a dose-dependent manner [Matsueda et al. 2008]. Although ramosetron has an affinity for the 5-HT3 receptor more than three times that of alosetron, the usual dose of 5 μg is very low compared with the equivalent usual dose of alosetron. Likewise, the potency of ondansetron is 3–10 times lower than that of alosetron [Clayton et al. 1999]. From these observations, it could be suggested that careful dosing at a very low level might avoid adverse events, including serious complications of ramosetron in the treatment of IBS-D.

Conclusion

Although there has been advancement in the treatment of IBS, current available therapies remain unsatisfactory. While 5-HT3 receptor antagonist is an approved target therapeutic agent with good efficacy in patients with IBS-D, it has been used in a limited number of patients due to its side effects and different clinical response between sexes. The 5-HT3 receptor antagonist available in Asia, ramosetron, shows good efficacy in terms of stool consistency, abdominal pain/discomfort, and health-related quality of life. In addition, ramosetron is associated with a lower incidence of constipation compared with other 5-HT3 receptor antagonists. Furthermore, ischemic colitis, which had been observed with alosetron, seems unlikely to be caused by ramosetron. Lastly, ramosetron would be effective in female patients as well as in male patients. Although further large prospective studies are needed to assess whether ramosetron is effective for female patients with IBS-D and to evaluate its long-term safety, ramosetron appears to be one of the most promising agents for patients with IBS-D.

Footnotes

Conflict of interest statement: The authors declare that there is no conflict of interest.

Funding: This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

Contributor Information

Yang Won Min, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

Poong-Lyul Rhee, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 135-710, Korea.

References

  1. Andresen V., Montori V., Keller J., West C., Layer P., Camilleri M. (2008) Effects of 5-hydroxytryptamine (serotonin) type 3 antagonists on symptom relief and constipation in nonconstipated irritable bowel syndrome: a systematic review and meta-analysis of randomized controlled trials. Clin Gastroenterol Hepatol 6: 545–555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bardhan K., Bodemar G., Geldof H., Schutz E., Heath A., Mills J., et al. (2000) A double-blind, randomized, placebo-controlled dose-ranging study to evaluate the efficacy of alosetron in the treatment of irritable bowel syndrome. Aliment Pharmacol Ther 14: 23–34. [DOI] [PubMed] [Google Scholar]
  3. Beattie D., Smith J. (2008) Serotonin pharmacology in the gastrointestinal tract: a review. Naunyn Schmiedebergs Arch Pharmacol 377: 181–203. [DOI] [PubMed] [Google Scholar]
  4. Berman S., Munakata J., Naliboff B., Chang L., Mandelkern M., Silverman D., et al. (2000) Gender differences in regional brain response to visceral pressure in IBS patients. Eur J Pain 4: 157–172. [DOI] [PubMed] [Google Scholar]
  5. Bjornsson E., Chey W., Ladabaum U., Woods M., Hooper F., Owyang C., et al. (1998) Differential 5-HT3 mediation of human gastrocolonic response and colonic peristaltic reflex. Am J Physiol 275: G498–G505. [DOI] [PubMed] [Google Scholar]
  6. Bush T., Spencer N., Watters N., Sanders K., Smith T. (2001) Effects of alosetron on spontaneous migrating motor complexes in murine small and large bowel in vitro. Am J Physiol Gastrointest Liver Physiol 281: G974–G983. [DOI] [PubMed] [Google Scholar]
  7. Camilleri M. (2005) Mechanisms in IBS: something old, something new, something borrowed. Neurogastroenterol Motil 17: 311–316. [DOI] [PubMed] [Google Scholar]
  8. Camilleri M. (2012) Peripheral mechanisms in irritable bowel syndrome. N Engl J Med 367: 1626–1635. [DOI] [PubMed] [Google Scholar]
  9. Camilleri M., Atanasova E., Carlson P., Ahmad U., Kim H., Viramontes B., et al. (2002) Serotonin-transporter polymorphism pharmacogenetics in diarrhea-predominant irritable bowel syndrome. Gastroenterology 123: 425–432. [DOI] [PubMed] [Google Scholar]
  10. Chang L., Ameen V., Dukes G., McSorley D., Carter E., Mayer E. (2005) A dose-ranging, phase II study of the efficacy and safety of alosetron in men with diarrhea-predominant IBS. Am J Gastroenterol 100: 115–123. [DOI] [PubMed] [Google Scholar]
  11. Clayton N., Sargent R., Butler A., Gale J., Maxwell M., Hunt A., et al. (1999) The pharmacological properties of the novel selective 5-HT3 receptor antagonist, alosetron, and its effects on normal and perturbed small intestinal transit in the fasted rat. Neurogastroenterol Motil 11: 207–217. [DOI] [PubMed] [Google Scholar]
  12. Cooke H. (2000) Neurotransmitters in neuronal reflexes regulating intestinal secretion. Ann N Y Acad Sci 915: 77–80. [DOI] [PubMed] [Google Scholar]
  13. Crowell M. (2004) Role of serotonin in the pathophysiology of the irritable bowel syndrome. Br J Pharmacol 141: 1285–1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Darvish-Damavandi M., Nikfar S., Abdollahi M. (2010) A systematic review of efficacy and tolerability of mebeverine in irritable bowel syndrome. World J Gastroenterol 16: 547–553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Farber L., Haus U., Spath M., Drechsler S. (2004) Physiology and pathophysiology of the 5-HT3 receptor. Scand J Rheumatol Suppl 119: 2–8. [PubMed] [Google Scholar]
  16. Fukudo S., Ida M., Akiho H., Nakashima Y., Matsueda K. (2014) Effect of ramosetron on stool consistency in male patients with irritable bowel syndrome with diarrhea. Clin Gastroenterol Hepatol 12: 953–959 e954. [DOI] [PubMed] [Google Scholar]
  17. Funatsu T., Takeuchi A., Hirata T., Keto Y., Akuzawa S., Sasamata M. (2007) Effect of ramosetron on conditioned emotional stress-induced colonic dysfunction as a model of irritable bowel syndrome in rats. Eur J Pharmacol 573: 190–195. [DOI] [PubMed] [Google Scholar]
  18. Garsed K., Chernova J., Hastings M., Lam C., Marciani L., Singh G., et al. (2014) A randomised trial of ondansetron for the treatment of irritable bowel syndrome with diarrhoea. Gut 63: 1617–1625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Gershon M. (1999) The enteric nervous system: a second brain. Hosp Pract (1995) 34: 31–32, 35–38, 41–42 passim. [DOI] [PubMed] [Google Scholar]
  20. Gershon M., Tack J. (2007) The serotonin signaling system: from basic understanding to drug development for functional GI disorders. Gastroenterology 132: 397–414. [DOI] [PubMed] [Google Scholar]
  21. Goldberg P., Kamm M., Setti-Carraro P., Van Der Sijp J., Roth C. (1996) Modification of visceral sensitivity and pain in irritable bowel syndrome by 5-HT3 antagonism (ondansetron). Digestion 57: 478–483. [DOI] [PubMed] [Google Scholar]
  22. Grasberger H., Chang L., Shih W., Presson A., Sayuk G., Newberry R., et al. (2013) Identification of a functional TPH1 polymorphism associated with irritable bowel syndrome bowel habit subtypes. Am J Gastroenterol 108: 1766–1774. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Hasler W. (2011) Traditional thoughts on the pathophysiology of irritable bowel syndrome. Gastroenterol Clin North Am 40: 21–43. [DOI] [PubMed] [Google Scholar]
  24. Hirata T., Keto Y., Funatsu T., Akuzawa S., Sasamata M. (2007) Evaluation of the pharmacological profile of ramosetron, a novel therapeutic agent for irritable bowel syndrome. J Pharmacol Sci 104: 263–273. [DOI] [PubMed] [Google Scholar]
  25. Hirata T., Keto Y., Nakata M., Takeuchi A., Funatsu T., Akuzawa S., et al. (2008) Effects of serotonin 5-HT3 receptor antagonists on stress-induced colonic hyperalgesia and diarrhoea in rats: a comparative study with opioid receptor agonists, a muscarinic receptor antagonist and a synthetic polymer. Neurogastroenterol Motil 20: 557–565. [DOI] [PubMed] [Google Scholar]
  26. Kilpatrick L., Labus J., Coveleskie K., Hammer C., Rappold G., Tillisch K., et al. (2011) The HTR3a polymorphism C. -42C>T is associated with amygdala responsiveness in patients with irritable bowel syndrome. Gastroenterology 140: 1943–1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Koch K., Corrigan B., Manzo J., James C., Scott R., Stead A., et al. (2004) Alosetron repeat dose pharmacokinetics, effects on enzyme activities, and influence of demographic factors. Aliment Pharmacol Ther 20: 223–230. [DOI] [PubMed] [Google Scholar]
  28. Koch K., Palmer J., Noordin N., Tomlinson J., Baidoo C. (2002) Sex and age differences in the pharmacokinetics of alosetron. Br J Clin Pharmacol 53: 238–242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Kumano H., Kaiya H., Yoshiuchi K., Yamanaka G., Sasaki T., Kuboki T. (2004) Comorbidity of irritable bowel syndrome, panic disorder, and agoraphobia in a Japanese representative sample. Am J Gastroenterol 99: 370–376. [DOI] [PubMed] [Google Scholar]
  30. Lee K., Kim N., Kwon J., Huh K., Lee O., Lee J., et al. (2011) Efficacy of ramosetron in the treatment of male patients with irritable bowel syndrome with diarrhea: a multicenter, randomized clinical trial, compared with mebeverine. Neurogastroenterol Motil 23: 1098–1104. [DOI] [PubMed] [Google Scholar]
  31. Longstreth G., Thompson W., Chey W., Houghton L., Mearin F., Spiller R. (2006) Functional bowel disorders. Gastroenterology 130: 1480–1491. [DOI] [PubMed] [Google Scholar]
  32. Lovell R., Ford A. (2012) Global prevalence of and risk factors for irritable bowel syndrome: a meta-analysis. Clin Gastroenterol Hepatol 10: 712–721 e714. [DOI] [PubMed] [Google Scholar]
  33. Matsueda K., Harasawa S., Hongo M., Hiwatashi N., Sasaki D. (2008) A randomized, double-blind, placebo-controlled clinical trial of the effectiveness of the novel serotonin type 3 receptor antagonist ramosetron in both male and female Japanese patients with diarrhea-predominant irritable bowel syndrome. Scand J Gastroenterol 43: 1202–1211. [DOI] [PubMed] [Google Scholar]
  34. Mayer E., Berman S., Derbyshire S., Suyenobu B., Chang L., Fitzgerald L., et al. (2002) The effect of the 5-HT3 receptor antagonist, alosetron, on brain responses to visceral stimulation in irritable bowel syndrome patients. Aliment Pharmacol Ther 16: 1357–1366. [DOI] [PubMed] [Google Scholar]
  35. Miyata K., Ito H., Fukudo S. (1998) Involvement of the 5-HT3 receptor in CRH-induce defecation in rats. Am J Physiol 274: G827–G831. [DOI] [PubMed] [Google Scholar]
  36. Naliboff B., Berman S., Chang L., Derbyshire S., Suyenobu B., Vogt B., et al. (2003) Sex-related differences in IBS patients: central processing of visceral stimuli. Gastroenterology 124: 1738–1747. [DOI] [PubMed] [Google Scholar]
  37. Niesler B., Kapeller J., Fell C., Atkinson W., Moller D., Fischer C., et al. (2010) 5-HTTLPR and STin2 polymorphisms in the serotonin transporter gene and irritable bowel syndrome: effect of bowel habit and sex. Eur J Gastroenterol Hepatol 22: 856–861. [DOI] [PubMed] [Google Scholar]
  38. Peroutka S. (1994) 5-Hydroxytryptamine receptors in vertebrates and invertebrates: why are there so many? Neurochem Int 25: 533–536. [DOI] [PubMed] [Google Scholar]
  39. Prior A., Read N. (1993) Reduction of rectal sensitivity and post-prandial motility by granisetron, a 5 HT3-receptor antagonist, in patients with irritable bowel syndrome. Aliment Pharmacol Ther 7: 175–180. [DOI] [PubMed] [Google Scholar]
  40. Rabasseda X. (2002) Ramosetron, a 5-HT3 receptor antagonist for the control of nausea and vomiting. Drugs Today (Barc) 38: 75–89. [DOI] [PubMed] [Google Scholar]
  41. Smith T., Park K., Hennig G. (2014) Colonic migrating motor complexes, high amplitude propagating contractions, neural reflexes and the importance of neuronal and mucosal serotonin. J Neurogastroenterol Motil 20: 423–446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Steadman C., Talley N., Phillips S., Zinsmeister A. (1992) Selective 5-hydroxytryptamine type 3 receptor antagonism with ondansetron as treatment for diarrhea-predominant irritable bowel syndrome: a pilot study. Mayo Clin Proc 67: 732–738. [DOI] [PubMed] [Google Scholar]
  43. Talley N., Phillips S., Haddad A., Miller L., Twomey C., Zinsmeister A., et al. (1990) GR 38032F (ondansetron), a selective 5HT3 receptor antagonist, slows colonic transit in healthy man. Dig Dis Sci 35: 477–480. [DOI] [PubMed] [Google Scholar]
  44. Tong K., Nicandro J., Shringarpure R., Chuang E., Chang L. (2013) A 9-year evaluation of temporal trends in alosetron postmarketing safety under the risk management program. Therap Adv Gastroenterol 6: 344–357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Viramontes B., Camilleri M., Mckinzie S., Pardi D., Burton D., Thomforde G. (2001) Gender-related differences in slowing colonic transit by a 5-HT3 antagonist in subjects with diarrhea-predominant irritable bowel syndrome. Am J Gastroenterol 96: 2671–2676. [DOI] [PubMed] [Google Scholar]
  46. Yamamoto C., Murakami H., Koyabu N., Takanaga H., Matsuo H., Uchiumi T., et al. (2002) Contribution of p-glycoprotein to efflux of ramosetron, a 5-HT3 receptor antagonist, across the blood-brain barrier. J Pharm Pharmacol 54: 1055–1063. [DOI] [PubMed] [Google Scholar]

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