Table 2.
Mean number of CFU / 500 cells plated | ||||||
---|---|---|---|---|---|---|
Culture Condition |
CFU-GM | BFU-E | CFU-mix | Total CFU | Plating Efficiency (%) |
Fold Expansion of CFU-mix |
Day 0
Primary MPB |
64.0 ± 11.8 | 75.2 ± 0.2 | 2.7 ± 0.8 | 141.8 ± 10.9 | 28.4 ± 2.2 | n/a |
Day 9
Cytokine A |
38.2 ± 8.7 | 3.5 ± 0.8 | 0 | 41.7 ± 9.4 | 8.3 ± 1.9 | 0 |
Day 9
5azaD/TSA + Cytokine A |
49.2 ± 6.3 | 8.2 ± 1.1 | 3 ± 0 | 60.3 ± 6.3 | 10.4 ± 1.9 | 10.1 ± 0.5 |
Day 9
Cytokine B |
14.0 ± 0.7 | 1.3 ± 0.2 | 0 | 15.3 ± 0.5 | 3.1 ± 0.1 | 0 |
Day 9
5azaD/TSA + Cytokine B |
33.5 ± 9.2 | 6.5 ± 0.4 | 2.25 ± 0.2 | 42.3 ± 9.0 | 8.5 ± 1.8 | 13.0 ± 0.2 |
MPB = mobilized peripheral blood; CFU = colony-forming unit; BFU = burst-forming unit; 5azaD = 5-aza-2’-deoxyctidine (5azaD); TSA = trichostatin A Cytokine A and B represent cytokine conditions with the highest and lowest expansion of CD34+CD90+ cord blood cells, respectively1