Skip to main content
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1960 Feb;39(2):364–377. doi: 10.1172/JCI104047

CHARACTERIZATION OF URINARY STEROIDS IN ADRENAL HYPERPLASIA: ISOLATION OF METABOLITES OF CORTISOL, COMPOUND S, AND DESOXYCORTICOSTERONE FROM A NORMOTENSIVE PATIENT WITH ADRENOGENITAL SYNDROME *

Hortense M Gandy 1,, E Henry Keutmann 1, Anthony J Izzo 1
PMCID: PMC441814  PMID: 13826293

Full text

PDF
364

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARNETT H. L., McNAMARA H. Electrolyte balances in a male infant with adrenocortical insufficiency and virilism; the effect of desoxycorticosterone acetate and salt therapy with special reference to potassium. J Clin Invest. 1949 Nov;28(6 Pt 2):1498–1506. doi: 10.1172/JCI102215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BARTTER F. C., ALBRIGHT F., FORBES A. P., LEAF A., DEMPSEY E., CARROLL E. The effects of adrenocorticotropic hormone and cortisone in the adrenogenital syndrome associated with congenital adrenal hyperplasia: an attempt to explain and correct its disordered hormonal pattern. J Clin Invest. 1951 Mar;30(3):237–251. doi: 10.1172/JCI102438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BIRKE G., DICZFALUSY E., PLANTIN L. O., ROBBE H., WESTMAN A. Familial congenital hyperplasia of the adrenal cortex. Acta Endocrinol (Copenh) 1958 Sep;29(1):55–69. doi: 10.1530/acta.0.0290055. [DOI] [PubMed] [Google Scholar]
  4. BIRKE G. The effect of Reichstein's substance S on the excretion of 17-ketosteroids. Acta Endocrinol (Copenh) 1954 Jan;15(1):17–24. doi: 10.1530/acta.0.0150017. [DOI] [PubMed] [Google Scholar]
  5. BONGIOVANNI A. M. Adrenogenital syndrome: uncomplicated and hypertensive forms. Pediatrics. 1958 Apr;21(4):661–667. [PubMed] [Google Scholar]
  6. BONGIOVANNI A. M., EBERLEIN W. R. Clinical and metabolic variations in the adrenogenital syndrome. Pediatrics. 1955 Nov;16(5):628–636. [PubMed] [Google Scholar]
  7. BONGIOVANNI A. M., EBERLEIN W. R. Plasma and urinary corticosteroids in the hypertensive form of congenital adrenal hyperplasia. J Biol Chem. 1956 Nov;223(1):85–94. [PubMed] [Google Scholar]
  8. BONGIOVANNI A. M. The detection of pregnandiol and pregnantriol in the urine of patients with adrenal hyperplasia, suppression with cortisone; preliminary report. Bull Johns Hopkins Hosp. 1953 Mar;92(3):244–251. [PubMed] [Google Scholar]
  9. BRICAIRE H., GRAVELEAU J., LAUDAT P. Un nouveau cas d'hyperplasie congénitale des surrénales à forme hypertensive; isolement du composé S. Ann Endocrinol (Paris) 1958 Jul-Aug;19(4):725–733. [PubMed] [Google Scholar]
  10. BROOKS R. V. A method for the quantitative fractionation of urinary 17-oxo steroids with some observations on steroid excretion during administration of ACTH and in the adrenogenital syndrome. Biochem J. 1958 Jan;68(1):50–57. doi: 10.1042/bj0680050. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. BULASCHENKO H., DOHAN F. C., RICHARDSON E. M., TOUCHSTONE J. C. The excretion of pregnane-3alpha, 17alpha, 21-triol-20-one (tetrahydro S) in normal and pathologic urine. J Clin Endocrinol Metab. 1957 Feb;17(2):250–255. doi: 10.1210/jcem-17-2-250. [DOI] [PubMed] [Google Scholar]
  12. BURTON R. B., ZAFFARONI A., KEUTMANN E. H. Corticosteroids in urine of normal persons determined by paper chromatography. J Biol Chem. 1951 Dec;193(2):769–779. [PubMed] [Google Scholar]
  13. BUSH I. E. Chromatography of steroids and sterols. Br Med Bull. 1954;10(3):229–236. doi: 10.1093/oxfordjournals.bmb.a069427. [DOI] [PubMed] [Google Scholar]
  14. BUSH I. E., WILLOUGHBY M. The excretion of allo tetrahydrocortisol in human urine. Biochem J. 1957 Dec;67(4):689–700. doi: 10.1042/bj0670689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. CHRISTY N. P., WALLACE E. Z., JAILER J. W. The effect of intravenously-administered ACTH on plasma 17,21-dihydroxy-20-ketosteroids in normal individuals and in patients with disorders of the adrenal cortex. J Clin Invest. 1955 Jun;34(6):899–906. doi: 10.1172/JCI103146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. CO TUI Peroral use of protein hydrolysates. Rev Gastroenterol. 1950 Mar;17(3):184–188. [PubMed] [Google Scholar]
  17. COX R. I. Separation, detection and estimation of C21 17:20-dihydroxy-20-methylsteroids. Nature. 1958 Mar 1;181(4609):638–639. doi: 10.1038/181638b0. [DOI] [PubMed] [Google Scholar]
  18. DAVIES D. F., CLARK H. E. A hypertensive syndrome with relative adrenal cortical overactivity. Circulation. 1950 Oct;2(4):494–504. doi: 10.1161/01.cir.2.4.494. [DOI] [PubMed] [Google Scholar]
  19. DE COURCY C. A trichloracetic acid reagent for the detection of pregnane-3alpha:17alpha:20-triols on paper chromatograms. J Endocrinol. 1956 Oct;14(2):164–170. doi: 10.1677/joe.0.0140164. [DOI] [PubMed] [Google Scholar]
  20. DYRENFURTH I., SYBULSKI S., NOTCHEV V., BECK J. C., VENNING E. H. Urinary corticosteroid excretion patterns in patients with adrenocortical dysfunction. J Clin Endocrinol Metab. 1958 Apr;18(4):391–408. doi: 10.1210/jcem-18-4-391. [DOI] [PubMed] [Google Scholar]
  21. EBERLEIN W. R., BONGIOVANNI A. M. Congenital adrenal hyperplasia with hypertension: unusual steroid pattern in blood and urine. J Clin Endocrinol Metab. 1955 Dec;15(12):1531–1534. doi: 10.1210/jcem-15-12-1531. [DOI] [PubMed] [Google Scholar]
  22. EBERLEIN W. R., BONGIOVANNI A. M. Partial characterization of urinary adrenocortical steroids in adrenal hyperplasia. J Clin Invest. 1955 Aug;34(8):1337–1343. doi: 10.1172/JCI103181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. FINKELSTEIN M., COX R. I. Method for simultaneous estimation of pregnane-3alpha, 17alpha, 20alpha-triol and pregnane-3alpha, 17alpha, 20alpha-triol-11-one in urine. Proc Soc Exp Biol Med. 1957 Jun;95(2):297–300. doi: 10.3181/00379727-95-23199. [DOI] [PubMed] [Google Scholar]
  24. FUKUSHIMA D. K., GALLAGHER T. F. Steroid isolation studies in congenital adrenal hyperplasia. J Biol Chem. 1957 Nov;229(1):85–92. [PubMed] [Google Scholar]
  25. HAINES W. J., KARNEMAAT J. N. Chromatographic separation of the steroids of the adrenal gland. Methods Biochem Anal. 1954;1:171–204. doi: 10.1002/9780470110171.ch7. [DOI] [PubMed] [Google Scholar]
  26. HECHTER O., PINCUS G. Genesis of the adrenocortical secretion. Physiol Rev. 1954 Jul;34(3):459–496. doi: 10.1152/physrev.1954.34.3.459. [DOI] [PubMed] [Google Scholar]
  27. IZZO A. J., KEUTMANN E. H., BURTON R. B. Special considerations in the use of blue tetrazolium for the measurement of alpha-ketols in mixtures with other steroids. J Clin Endocrinol Metab. 1957 Jul;17(7):889–901. doi: 10.1210/jcem-17-7-889. [DOI] [PubMed] [Google Scholar]
  28. JAILER J. W., GOLD J. J., VANDE WIELE R., LIEBERMAN S. 17alpha-hydroxyprogesterone and 21-desoxyhydrocortisone; their metabolism and possible role in congenital adrenal virilism. J Clin Invest. 1955 Nov;34(11):1639–1646. doi: 10.1172/JCI103217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. JAILER J. W. Virilism. Bull N Y Acad Med. 1953 May;29(5):377–394. [PMC free article] [PubMed] [Google Scholar]
  30. KELLEY V. C., ELY R. S., RAILE R. B. Hormone patterns in patients with congenital adrenal hyperplasia. Pediatrics. 1953 Nov;12(5):541–548. [PubMed] [Google Scholar]
  31. KRITCHEVSKY D., KIRK M. R. Detection of steroids in paper chromatography. Arch Biochem Biophys. 1952 Feb;35(2):346–351. doi: 10.1016/s0003-9861(52)80015-3. [DOI] [PubMed] [Google Scholar]
  32. Lewis R. A., Wilkins L. THE EFFECT OF ADRENOCORTICOTROPHIC HORMONE IN CONGENITAL ADRENAL HYPERPLASIA WITH VIRILISM AND IN CUSHING'S SYNDROME TREATED WITH METHYL TESTOSTERONE. J Clin Invest. 1949 Mar;28(2):394–400. doi: 10.1172/JCI102082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. MASON A. S. Adrenal virilism: abnormal response to ACTH. Proc R Soc Med. 1951 Feb;44(2):162–163. [PMC free article] [PubMed] [Google Scholar]
  34. REICH H., NELSON D. H., ZAFFARONI A. Isolation of 17-hydroxycorticosterone from blood obtained from adrenal veins of dogs. J Biol Chem. 1950 Nov;187(1):411–417. [PubMed] [Google Scholar]
  35. RICHARDSON E. M., TOUCHSTONE J. C., DOHAN F. C. Urinary alpha-ketolic steroid metabolites of cortical hormones administered to subjects with adrenal cortical insufficiency. J Clin Invest. 1955 Feb;34(2):285–301. doi: 10.1172/JCI103082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. ROMANOFF E. B., HUNT C. A. Detection of C-21 glycerol side chain steroids on paper chromatograms. Endocrinology. 1955 Oct;57(4):499–502. doi: 10.1210/endo-57-4-499. [DOI] [PubMed] [Google Scholar]
  37. ROMANOFF L. P., SEELYE J., RODRIGUEZ R., PINCUS G. The regular occurrence of 3 alpha-allotetrahydrocortisol (3 alpha, 11 beta, 17 alpha-21-tetrahydroxyallopregnan-20-one) in human urine. J Clin Endocrinol Metab. 1957 Mar;17(3):434–437. doi: 10.1210/jcem-17-3-434. [DOI] [PubMed] [Google Scholar]
  38. SHEPARD T. H., CLAUSEN S. W. Case of adrenogenital syndrome with hypertension treated with cortisone. Pediatrics. 1951 Dec;8(6):805–811. [PubMed] [Google Scholar]
  39. TAYLOR W. Isolation of oxo-steroids under neutral conditions. Nature. 1958 Dec 20;182(4651):1735–1735. doi: 10.1038/1821735a0. [DOI] [PubMed] [Google Scholar]
  40. TOUCHSTONE J. C., RICHARDSON E. M., BULASCHENKO H., LANDOLT I., DOHAN F. C. Isolation of pregnane-3-alpha, 17-alpha, 21-triol-20-one (tetrahydro compound S) from the urine of a woman with metastatic adrenocortical carcinoma. J Clin Endocrinol Metab. 1954 Jun;14(6):676–678. doi: 10.1210/jcem-14-6-676. [DOI] [PubMed] [Google Scholar]
  41. Thorn G. W., Howard R. P., Emerson K. TREATMENT OF ADDISON'S DISEASE WITH DESOXY-CORTICOSTERONE ACETATE, A SYNTHETIC ADRENAL CORTICAL HORMONE (PRELIMINARY REPORT). J Clin Invest. 1939 Jul;18(4):449–467. doi: 10.1172/JCI101060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. WHITE F. P., SUTTON L. E. Adrenogenital syndrome with associated episodes of hypoglycemia. J Clin Endocrinol Metab. 1951 Nov;11(11):1395–1402. doi: 10.1210/jcem-11-11-1395. [DOI] [PubMed] [Google Scholar]
  43. WILKINS L., CRIGLER J. F., Jr, SILVERMAN S. H., GARDNER L. I., MIGEON C. J. Further studies on the treatment of congenital adrenal hyperplasia with cortisone. III. The control of hypertension with cortisone, with a discussion of variations in the type of congenital adrenal hyperplasia and report of a case with probable defect of carbohydrate-regulating hormones. J Clin Endocrinol Metab. 1952 Aug;12(8):1015–1030. doi: 10.1210/jcem-12-8-1015. [DOI] [PubMed] [Google Scholar]
  44. WILKINS L. The diagnosis of the adrenogenital syndrome and its treatment with cortisone. J Pediatr. 1952 Dec;41(6):860–874. doi: 10.1016/s0022-3476(52)80306-3. [DOI] [PubMed] [Google Scholar]
  45. WILSON H., FAIRBANKS R. A micromethod for the detection and assay of steroid C21 17-hydroxy-alpha-glycols. Arch Biochem Biophys. 1955 Feb;54(2):457–466. doi: 10.1016/0003-9861(55)90058-8. [DOI] [PubMed] [Google Scholar]
  46. ZAFFARONI A., BURTON R. B. Identification of corticosteroids of beef adrenal extract by paper chromatography. J Biol Chem. 1951 Dec;193(2):749–767. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES