Table 3.
Compound | Initial luminal tracer concentration (μM) | Final abluminal tracer concentration (μM) | Papp (10−6 cm/s) |
---|---|---|---|
GRL-04810 | 100 | 3.33 ±0.70 | 50.38 ± 10.61 |
GRL-05010 | 100 | 4.01 ± 0.27 | 60.84 ± 3.97 |
DRV | 100 | 0.62 ± 0.15 | 9.32 ± 2.25 |
In the in vitro model using a triple co-culture of rat astrocytes, pericytes and monkey endothelial cells, GRL-04810, GRL-05010, DRV (all 100 μM) were added to the luminal interface (termed blood side) of duplicate wells. The mathematical formula used for the calculation of Papp is described in Materials and Methods. Results show average values ±1 S.D. of duplicated determinations.