Abstract
No information is available on the rate of blood flow in transplanted islets. In this study, adult rats were partially depancreatized, and islets from the excised pancreas were then isolated, maintained for 7 d in tissue culture, and subsequently transplanted back to the animal, beneath the renal capsule. Some rats were rendered diabetic with streptozotocin before transplantation. A month after transplantation the blood flow of the grafts was measured by a microsphere technique. Autotransplantation to streptozotocin-diabetic rats of approximately 500 islets did not revert the hyperglycemia, and the blood flow of these grafts was approximately 25% of that in the normoglycemic-transplanted rats. However, in insulin-treated diabetic rats the blood flow of the pancreatic graft was similar to that in the normoglycemic rats. The present results suggest that the blood flow in transplanted islets is markedly diminished by hyperglycemia and that this can be enhanced by insulin administration.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andersson A. Isolated mouse pancreatic islets in culture: effects of serum and different culture media on the insulin production of the islets. Diabetologia. 1978 Jun;14(6):397–404. doi: 10.1007/BF01228134. [DOI] [PubMed] [Google Scholar]
- Ballinger W. F., Lacy P. E. Transplantation of intact pancreatic islets in rats. Surgery. 1972 Aug;72(2):175–186. [PubMed] [Google Scholar]
- Bonner-Weir S., Trent D. F., Weir G. C. Partial pancreatectomy in the rat and subsequent defect in glucose-induced insulin release. J Clin Invest. 1983 Jun;71(6):1544–1553. doi: 10.1172/JCI110910. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown J., Molnar I. G., Clark W., Mullen Y. Control of experimental diabetes mellitus in rats by transplantation of fetal pancreases. Science. 1974 Jun 28;184(4144):1377–1379. doi: 10.1126/science.184.4144.1377. [DOI] [PubMed] [Google Scholar]
- Griffith R. C., Scharp D. W., Hartman B. K., Ballinger W. F., Lacy P. E. A morphologic study of intrahepatic portal-vein islet isografts. Diabetes. 1977 Mar;26(3):201–214. doi: 10.2337/diab.26.3.201. [DOI] [PubMed] [Google Scholar]
- Heding L. G. Determination of total serum insulin (IRI) in insulin-treated diabetic patients. Diabetologia. 1972 Aug;8(4):260–266. doi: 10.1007/BF01225569. [DOI] [PubMed] [Google Scholar]
- Jansson L. Dissociation between pancreatic islet blood flow and insulin release in the rat. Acta Physiol Scand. 1985 Jun;124(2):223–228. doi: 10.1111/j.1748-1716.1985.tb07655.x. [DOI] [PubMed] [Google Scholar]
- Jansson L., Hellerström C. A rapid method of visualizing the pancreatic islets for studies of islet capillary blood flow using non-radioactive microspheres. Acta Physiol Scand. 1981;113(3):371–374. doi: 10.1111/j.1748-1716.1981.tb06909.x. [DOI] [PubMed] [Google Scholar]
- Jansson L., Hellerström C. Stimulation by glucose of the blood flow to the pancreatic islets of the rat. Diabetologia. 1983 Jul;25(1):45–50. doi: 10.1007/BF00251896. [DOI] [PubMed] [Google Scholar]
- Jansson L., Sandler S. Pancreatic islet circulation in relation to the diabetogenic action of streptozotocin in the rat. Endocrinology. 1985 Mar;116(3):896–900. doi: 10.1210/endo-116-3-896. [DOI] [PubMed] [Google Scholar]
- Lifson N., Lassa C. V., Dixit P. K. Relation between blood flow and morphology in islet organ of rat pancreas. Am J Physiol. 1985 Jul;249(1 Pt 1):E43–E48. doi: 10.1152/ajpendo.1985.249.1.E43. [DOI] [PubMed] [Google Scholar]
- Sutherland D. E. Pancreas and islet transplantation. I. Experimental studies. Diabetologia. 1981 Mar;20(3):161–185. doi: 10.1007/BF00252624. [DOI] [PubMed] [Google Scholar]