Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1987 Aug;80(2):325–332. doi: 10.1172/JCI113076

B lymphocyte reconstitution after human bone marrow transplantation. Leu-1 antigen defines a distinct population of B lymphocytes.

J H Antin, K A Ault, J M Rappeport, B R Smith
PMCID: PMC442241  PMID: 3112184

Abstract

Differences in the expression of Leu-1 (CD5) define two populations of recovering B cells after human marrow transplantation, Leu-1+ and Leu-1- B cells. The Leu-1+ B cells were polyclonal, of donor origin, and did not express detectable interleukin 2 receptor. Leu-1+ B cells generally appeared 2-4 wk after marrow grafting and often preceded the recovery of Leu-1- B cells. Acute and chronic graft vs. host disease (GvHD) resulted in the recovery of significantly fewer Leu-1+ B cells, whereas Leu-1- B cells were only decreased in acute GvHD. Multivariate analysis showed no significant effect of age, disease, prednisone or azathioprine, or ex vivo treatment of the marrow with anti-Leu-1 and complement on recovery of Leu-1+ and Leu-1- B cells, independent of the effects of GvHD. Leu-1+ B cells are a major lymphocyte population posttransplant. They may reflect a stage of differentiation of normal B cells or a separate B cell lineage.

Full text

PDF
325

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antin J. H., Emerson S. G., Martin P., Gadol N., Ault K. A. Leu-1+ (CD5+) B cells. A major lymphoid subpopulation in human fetal spleen: phenotypic and functional studies. J Immunol. 1986 Jan;136(2):505–510. [PubMed] [Google Scholar]
  2. Ault K. A., Antin J. H., Ginsburg D., Orkin S. H., Rappeport J. M., Keohan M. L., Martin P., Smith B. R. Phenotype of recovering lymphoid cell populations after marrow transplantation. J Exp Med. 1985 Jun 1;161(6):1483–1502. doi: 10.1084/jem.161.6.1483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bofill M., Janossy G., Janossa M., Burford G. D., Seymour G. J., Wernet P., Kelemen E. Human B cell development. II. Subpopulations in the human fetus. J Immunol. 1985 Mar;134(3):1531–1538. [PubMed] [Google Scholar]
  4. Boumsell L., Coppin H., Pham D., Raynal B., Lemerle J., Dausset J., Bernard A. An antigen shared by a human T cell subset and B cell chronic lymphocytic leukemic cells. Distribution on normal and malignant lymphoid cells. J Exp Med. 1980 Jul 1;152(1):229–234. doi: 10.1084/jem.152.1.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Caligaris-Cappio F., Gobbi M., Bofill M., Janossy G. Infrequent normal B lymphocytes express features of B-chronic lymphocytic leukemia. J Exp Med. 1982 Feb 1;155(2):623–628. doi: 10.1084/jem.155.2.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gadol N., Ault K. A. Phenotypic and functional characterization of human Leu1 (CD5) B cells. Immunol Rev. 1986 Oct;93:23–34. doi: 10.1111/j.1600-065x.1986.tb01500.x. [DOI] [PubMed] [Google Scholar]
  7. Ginsburg D., Antin J. H., Smith B. R., Orkin S. H., Rappeport J. M. Origin of cell populations after bone marrow transplantation. Analysis using DNA sequence polymorphisms. J Clin Invest. 1985 Feb;75(2):596–603. doi: 10.1172/JCI111736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gobbi M., Caligaris-Cappio F., Janossy G. Normal equivalent cells of B cell malignancies: analysis with monoclonal antibodies. Br J Haematol. 1983 Jul;54(3):393–403. doi: 10.1111/j.1365-2141.1983.tb02114.x. [DOI] [PubMed] [Google Scholar]
  9. Hayakawa K., Hardy R. R., Herzenberg L. A., Herzenberg L. A. Progenitors for Ly-1 B cells are distinct from progenitors for other B cells. J Exp Med. 1985 Jun 1;161(6):1554–1568. doi: 10.1084/jem.161.6.1554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hayakawa K., Hardy R. R., Honda M., Herzenberg L. A., Steinberg A. D., Herzenberg L. A. Ly-1 B cells: functionally distinct lymphocytes that secrete IgM autoantibodies. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2494–2498. doi: 10.1073/pnas.81.8.2494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hayakawa K., Hardy R. R., Parks D. R., Herzenberg L. A. The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice. J Exp Med. 1983 Jan 1;157(1):202–218. doi: 10.1084/jem.157.1.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Korsmeyer S. J., Elfenbein G. J., Goldman C. K., Marshall S. L., Santos G. W., Waldmann T. A. B cell, helper T cell, and suppressor T cell abnormalities contribute to disordered immunoglobulin synthesis in patients following bone marrow transplantation. Transplantation. 1982 Feb;33(2):184–190. doi: 10.1097/00007890-198202000-00015. [DOI] [PubMed] [Google Scholar]
  13. Ledbetter J. A., Martin P. J., Spooner C. E., Wofsy D., Tsu T. T., Beatty P. G., Gladstone P. Antibodies to Tp67 and Tp44 augment and sustain proliferative responses of activated T cells. J Immunol. 1985 Oct;135(4):2331–2336. [PubMed] [Google Scholar]
  14. Lum L. G., Seigneuret M. C., Orcutt-Thordarson N., Noges J. E., Storb R. The regulation of immunoglobulin synthesis after HLA-identical bone marrow transplantation: VI. Differential rates of maturation of distinct functional groups within lymphoid subpopulations in patients after human marrow grafting. Blood. 1985 Jun;65(6):1422–1433. [PubMed] [Google Scholar]
  15. Lum L. G., Seigneuret M. C., Storb R. F., Witherspoon R. P., Thomas E. D. In vitro regulation of immunoglobulin synthesis after marrow transplantation. I. T-cell and B-cell deficiencies in patients with and without chronic graft-versus-host disease. Blood. 1981 Sep;58(3):431–439. [PubMed] [Google Scholar]
  16. Mauch P., Lipton J. M., Hamilton B., Obbagy J., Kudisch M., Nathan D., Hellman S. Lethal graft-versus-host disease: modification with allogeneic cultured donor cells. Blood. 1984 May;63(5):1112–1119. [PubMed] [Google Scholar]
  17. Miller R. A., Gralow J. The induction of Leu-1 antigen expression in human malignant and normal B cells by phorbol myristic acetate (PMA). J Immunol. 1984 Dec;133(6):3408–3414. [PubMed] [Google Scholar]
  18. Noel D. R., Witherspoon R. P., Storb R., Atkinson K., Doney K., Mickelson E. M., Ochs H. D., Warren R. P., Weiden P. L., Thomas E. D. Does graft-versus-host disease influence the tempo of immunologic recovery after allogeneic human marrow transplantation? An observation on 56 long-term survivors. Blood. 1978 Jun;51(6):1087–1105. [PubMed] [Google Scholar]
  19. Okumura K., Hayakawa K., Tada T. Cell-to-cell interaction controlled by immunoglobulin genes. Role of Thy-1-, Lyt-1+, Ig+ (B') cell in allotype-restricted antibody production. J Exp Med. 1982 Aug 1;156(2):443–453. doi: 10.1084/jem.156.2.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pahwa S. G., Pahwa R. N., Friedrich W., O'Reilly R. J., Good R. A. Abnormal humoral immune responses in peripheral blood lymphocyte cultures of bone marrow transplant recipients. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2663–2667. doi: 10.1073/pnas.79.8.2663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Parkman R., Rappeport J. M., Hellman S., Lipton J., Smith B., Geha R., Nathan D. G. Busulfan and total body irradiation as antihematopoietic stem cell agents in the preparation of patients with congenital bone marrow disorders for allogenic bone marrow transplantation. Blood. 1984 Oct;64(4):852–857. [PubMed] [Google Scholar]
  22. Peto R., Pike M. C., Armitage P., Breslow N. E., Cox D. R., Howard S. V., Mantel N., McPherson K., Peto J., Smith P. G. Design and analysis of randomized clinical trials requiring prolonged observation of each patient. II. analysis and examples. Br J Cancer. 1977 Jan;35(1):1–39. doi: 10.1038/bjc.1977.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Reinherz E. L., Geha R., Rappeport J. M., Wilson M., Penta A. C., Hussey R. E., Fitzgerald K. A., Daley J. F., Levine H., Rosen F. S. Reconstitution after transplantation with T-lymphocyte-depleted HLA haplotype-mismatched bone marrow for severe combined immunodeficiency. Proc Natl Acad Sci U S A. 1982 Oct;79(19):6047–6051. doi: 10.1073/pnas.79.19.6047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ringden O., Witherspoon R. P., Storb R., Ekelund E., Thomas E. D. Increased in vitro B-cell IgG secretion during acute graft-versus-host disease and infection. Observations in 50 human marrow transplant recipients. Blood. 1980 Feb;55(2):179–186. [PubMed] [Google Scholar]
  25. Saxon A., McIntyre R. E., Stevens R. H., Gale R. P. Lymphocyte dysfunction in chronic graft-versus-host disease. Blood. 1981 Oct;58(4):746–751. [PubMed] [Google Scholar]
  26. Sherr D. H., Dorf M. E. An idiotype-specific helper population that bears immunoglobulin, Ia, and Lyt-1 determinants. J Exp Med. 1984 Apr 1;159(4):1189–1200. doi: 10.1084/jem.159.4.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Shiobara S., Witherspoon R. P., Lum L. G., Storb R. Immunoglobulin synthesis after HLA-identical marrow grafting. V. The role of peripheral blood monocytes in the regulation of in vitro immunoglobulin secretion stimulated by pokeweed mitogen. J Immunol. 1984 Jun;132(6):2850–2856. [PubMed] [Google Scholar]
  28. Shulman H. M., Sullivan K. M., Weiden P. L., McDonald G. B., Striker G. E., Sale G. E., Hackman R., Tsoi M. S., Storb R., Thomas E. D. Chronic graft-versus-host syndrome in man. A long-term clinicopathologic study of 20 Seattle patients. Am J Med. 1980 Aug;69(2):204–217. doi: 10.1016/0002-9343(80)90380-0. [DOI] [PubMed] [Google Scholar]
  29. Sidman C. L., Marshall J. D., Masiello N. C., Roths J. B., Shultz L. D. Novel B-cell maturation factor from spontaneously autoimmune viable motheaten mice. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7199–7202. doi: 10.1073/pnas.81.22.7199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sidman C. L., Shultz L. D., Hardy R. R., Hayakawa K., Herzenberg L. A. Production of immunoglobulin isotypes by Ly-1+ B cells in viable motheaten and normal mice. Science. 1986 Jun 13;232(4756):1423–1425. doi: 10.1126/science.3487115. [DOI] [PubMed] [Google Scholar]
  31. Smith B. R., Guinan E. C., Parkman R., Ferrara J., Levey R. H., Nathan D. G., Rappeport J. M. Efficacy of a cyclophosphamide-procarbazine-antithymocyte serum regimen for prevention of graft rejection following bone marrow transplantation for transfused patients with aplastic anemia. Transplantation. 1985 Jun;39(6):671–673. [PubMed] [Google Scholar]
  32. Smith B. R., Parkman R., Lipton J., Nathan D. G., Rappeport J. M. Efficacy of a short course (four doses) of methotrexate following bone marrow transplantation for prevention of graft-versus-host disease. Transplantation. 1985 Mar;39(3):326–329. doi: 10.1097/00007890-198503000-00028. [DOI] [PubMed] [Google Scholar]
  33. Stanton T., Stevens T. L., Ledbetter J. A., Wofsy D. Anti-Ly-1 antibody induces interleukin 2 release from T cells. J Immunol. 1986 Mar 1;136(5):1734–1737. [PubMed] [Google Scholar]
  34. Thomas E. D., Storb R., Clift R. A., Fefer A., Johnson L., Neiman P. E., Lerner K. G., Glucksberg H., Buckner C. D. Bone-marrow transplantation (second of two parts). N Engl J Med. 1975 Apr 24;292(17):895–902. doi: 10.1056/NEJM197504242921706. [DOI] [PubMed] [Google Scholar]
  35. Thomas E., Storb R., Clift R. A., Fefer A., Johnson F. L., Neiman P. E., Lerner K. G., Glucksberg H., Buckner C. D. Bone-marrow transplantation (first of two parts). N Engl J Med. 1975 Apr 17;292(16):832–843. doi: 10.1056/NEJM197504172921605. [DOI] [PubMed] [Google Scholar]
  36. Thomas Y., Glickman E., DeMartino J., Wang J., Goldstein G., Chess L. Biologic functions of the OKT1 T cell surface antigen. I. The T1 molecule is involved in helper function. J Immunol. 1984 Aug;133(2):724–728. [PubMed] [Google Scholar]
  37. Tsudo M., Uchiyama T., Uchino H. Expression of Tac antigen on activated normal human B cells. J Exp Med. 1984 Aug 1;160(2):612–617. doi: 10.1084/jem.160.2.612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Waldmann T. A., Goldman C. K., Robb R. J., Depper J. M., Leonard W. J., Sharrow S. O., Bongiovanni K. F., Korsmeyer S. J., Greene W. C. Expression of interleukin 2 receptors on activated human B cells. J Exp Med. 1984 Nov 1;160(5):1450–1466. doi: 10.1084/jem.160.5.1450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Witherspoon R. P., Goehle S., Kretschmer M., Storb R. Regulation of immunoglobulin production after human marrow grafting. The role of helper and suppressor T cells in acute graft-versus-host disease. Transplantation. 1986 Mar;41(3):328–335. doi: 10.1097/00007890-198603000-00009. [DOI] [PubMed] [Google Scholar]
  40. Witherspoon R. P., Lum L. G., Storb R., Thomas E. D. In vitro regulation of immunoglobulin synthesis after human marrow transplantation. II. Deficient T and non-T lymphocyte function within 3-4 months of allogeneic, syngeneic, or autologous marrow grafting for hematologic malignancy. Blood. 1982 Apr;59(4):844–850. [PubMed] [Google Scholar]
  41. Witherspoon R. P., Storb R., Ochs H. D., Fluornoy N., Kopecky K. J., Sullivan K. M., Deeg J. H., Sosa R., Noel D. R., Atkinson K. Recovery of antibody production in human allogeneic marrow graft recipients: influence of time posttransplantation, the presence or absence of chronic graft-versus-host disease, and antithymocyte globulin treatment. Blood. 1981 Aug;58(2):360–368. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES