Abstract
Colony assays were performed for 50 patients with B cell precursor acute lymphoblastic leukemia (ALL). Blast colony formation was observed for 33 patients, and the plating efficiency (PE) showed a marked interpatient variation, which indicates a pronounced biological heterogeneity at the level of leukemic progenitor cells. Notably, the mean PE of leukemic B cell precursors from patients with a pseudodiploid or near-diploid karyotype with structural chromosomal abnormalities (SCA) was significantly higher than the mean PE of normal diploid or hyperdiploid cases. All patients who had SCA involving 7p13, 11q23-24, or 12p11-13, and patients with a Philadelphia chromosome had high PE values. The S phase percentage, expression of CD19 antigen, and relapse status were also correlated with PE. Significantly, colony blasts had slightly different surface marker profiles in each case and were common ALL antigen negative in 33% of cases, which indicates the existence of a marked immunological heterogeneity at the level of leukemic progenitor cells.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adams J. M. Oncogene activation by fusion of chromosomes in leukaemia. Nature. 1985 Jun 13;315(6020):541–542. doi: 10.1038/315542a0. [DOI] [PubMed] [Google Scholar]
- Arthur D. C., Bloomfield C. D., Lindquist L. L., Nesbit M. E., Jr Translocation 4; 11 in acute lymphoblastic leukemia: clinical characteristics and prognostic significance. Blood. 1982 Jan;59(1):96–99. [PubMed] [Google Scholar]
- Berger R., Bloomfield C. D., Sutherland G. R. Report of the Committee on Chromosome Rearrangements in Neoplasia and on Fragile Sites. Cytogenet Cell Genet. 1985;40(1-4):490–535. doi: 10.1159/000132181. [DOI] [PubMed] [Google Scholar]
- Bishop J. M. Viral oncogenes. Cell. 1985 Aug;42(1):23–38. doi: 10.1016/s0092-8674(85)80098-2. [DOI] [PubMed] [Google Scholar]
- Croce C. M., Thierfelder W., Erikson J., Nishikura K., Finan J., Lenoir G. M., Nowell P. C. Transcriptional activation of an unrearranged and untranslocated c-myc oncogene by translocation of a C lambda locus in Burkitt. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6922–6926. doi: 10.1073/pnas.80.22.6922. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dewald G. W., Kyle R. A., Hicks G. A., Greipp P. R. The clinical significance of cytogenetic studies in 100 patients with multiple myeloma, plasma cell leukemia, or amyloidosis. Blood. 1985 Aug;66(2):380–390. [PubMed] [Google Scholar]
- Erikson J., Nishikura K., ar-Rushdi A., Finan J., Emanuel B., Lenoir G., Nowell P. C., Croce C. M. Translocation of an immunoglobulin kappa locus to a region 3' of an unrearranged c-myc oncogene enhances c-myc transcription. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7581–7585. doi: 10.1073/pnas.80.24.7581. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Erikson J., ar-Rushdi A., Drwinga H. L., Nowell P. C., Croce C. M. Transcriptional activation of the translocated c-myc oncogene in burkitt lymphoma. Proc Natl Acad Sci U S A. 1983 Feb;80(3):820–824. doi: 10.1073/pnas.80.3.820. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greaves M. F. Analysis of the clinical and biological significance of lymphoid phenotypes in acute leukemia. Cancer Res. 1981 Nov;41(11 Pt 2):4752–4766. [PubMed] [Google Scholar]
- Haag D. Flow microfluorometric deoxyribonucleic acid (DNA) analysis supplementing routine histopathologic diagnosis of biopsy specimens. Lab Invest. 1980 Jan;42(1):85–90. [PubMed] [Google Scholar]
- Haag D., Goerttler K., Tschahargane C. The proliferative index (PI) of human breast cancer as obtained by flow cytometry. Pathol Res Pract. 1984 Mar;178(4):315–322. doi: 10.1016/S0344-0338(84)80020-5. [DOI] [PubMed] [Google Scholar]
- Heerema N. A., Palmer C. G., Baehner R. L. Karyotypic and clinical findings in a consecutive series of children with acute lymphocytic leukemia. Cancer Genet Cytogenet. 1985 Jun;17(2):165–179. doi: 10.1016/0165-4608(85)90027-5. [DOI] [PubMed] [Google Scholar]
- Izaguirre C. A., Curtis J., Messner H., McCulloch E. A. A colony assay for blast cell progenitors in non-B non-T (common) acute lymphoblastic leukemia. Blood. 1981 May;57(5):823–829. [PubMed] [Google Scholar]
- McCulloch E. A. Stem cells in normal and leukemic hemopoiesis (Henry Stratton Lecture, 1982). Blood. 1983 Jul;62(1):1–13. [PubMed] [Google Scholar]
- Miller D. R., Leikin S., Albo V., Sather H., Hammond D. Prognostic importance of morphology (FAB classification) in childhood acute lymphoblastic leukaemia (ALL). Br J Haematol. 1981 Jun;48(2):199–206. [PubMed] [Google Scholar]
- Minowada J., Tsubota T., Nakazawa S., Srivastava B. I., Huang C. C., Oshimura M., Sonta S., Han T., Sinks L. F., Sandberg A. A. Establishment and characterization of leukemic T-cell lines, B-cell lines, and null-cell line: a progress report on surface antigen study of fresh lymphatic leukemias in man. Haematol Blood Transfus. 1977;20:241–251. doi: 10.1007/978-3-642-66639-1_30. [DOI] [PubMed] [Google Scholar]
- Paolucci P., Rapson N. T., Layward L., Hayward A. R. Growth of pre-B cells in cultures of bone marrow from children with acute lymphoblastic leukaemia and other diseases. Clin Exp Immunol. 1981 Jan;43(1):143–148. [PMC free article] [PubMed] [Google Scholar]
- Ramsay N., LeBien T., Nesbit M., McGlave P., Weisdorf D., Kenyon P., Hurd D., Goldman A., Kim T., Kersey J. Autologous bone marrow transplantation for patients with acute lymphoblastic leukemia in second or subsequent remission: results of bone marrow treated with monoclonal antibodies BA-1, BA-2, and BA-3 plus complement. Blood. 1985 Sep;66(3):508–513. [PubMed] [Google Scholar]
- Sacchi N., Watson D. K., Guerts van Kessel A. H., Hagemeijer A., Kersey J., Drabkin H. D., Patterson D., Papas T. S. Hu-ets-1 and Hu-ets-2 genes are transposed in acute leukemias with (4;11) and (8;21) translocations. Science. 1986 Jan 24;231(4736):379–382. doi: 10.1126/science.3941901. [DOI] [PubMed] [Google Scholar]
- Sherr C. J., Rettenmier C. W., Sacca R., Roussel M. F., Look A. T., Stanley E. R. The c-fms proto-oncogene product is related to the receptor for the mononuclear phagocyte growth factor, CSF-1. Cell. 1985 Jul;41(3):665–676. doi: 10.1016/s0092-8674(85)80047-7. [DOI] [PubMed] [Google Scholar]
- Shtivelman E., Lifshitz B., Gale R. P., Canaani E. Fused transcript of abl and bcr genes in chronic myelogenous leukaemia. Nature. 1985 Jun 13;315(6020):550–554. doi: 10.1038/315550a0. [DOI] [PubMed] [Google Scholar]
- Smith S. D., Uyeki E. M., Lowman J. T. Colony formation in vitro by leukemic cells in acute lymphoblastic leukemia (ALL). Blood. 1978 Oct;52(4):712–718. [PubMed] [Google Scholar]
- Sporn M. B., Roberts A. B. Autocrine growth factors and cancer. 1985 Feb 28-Mar 6Nature. 313(6005):745–747. doi: 10.1038/313745a0. [DOI] [PubMed] [Google Scholar]
- Stong R. C., Kersey J. H. In vitro culture of leukemic cells in t(4;11) acute leukemia. Blood. 1985 Aug;66(2):439–443. [PubMed] [Google Scholar]
- Touw I., Delwel R., Bolhuis R., van Zanen G., Löwenberg B. Common and pre-B acute lymphoblastic leukemia cells express interleukin 2 receptors, and interleukin 2 stimulates in vitro colony formation. Blood. 1985 Sep;66(3):556–561. [PubMed] [Google Scholar]
- Uckun F. M., Gajl-Peczalska K. J., Kersey J. H., Houston L. L., Vallera D. A. Use of a novel colony assay to evaluate the cytotoxicity of an immunotoxin containing pokeweed antiviral protein against blast progenitor cells freshly obtained from patients with common B-lineage acute lymphoblastic leukemia. J Exp Med. 1986 Feb 1;163(2):347–368. doi: 10.1084/jem.163.2.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Uckun F. M., Gajl-Peczalska K., Meyers D. E., Ramsay N. C., Kersey J. H., Colvin M., Vallera D. A. Marrow purging in autologous bone marrow transplantation for T-lineage acute lymphoblastic leukemia: efficacy of ex vivo treatment with immunotoxins and 4-hydroperoxycyclophosphamide against fresh leukemic marrow progenitor cells. Blood. 1987 Jan;69(1):361–366. [PubMed] [Google Scholar]
- Uckun F. M., Ramakrishnan S., Haag D., Houston L. L. Ex vivo elimination of lymphoblastic leukemia cells from human marrow by mafosfamid. Leuk Res. 1985;9(1):83–95. doi: 10.1016/0145-2126(85)90023-2. [DOI] [PubMed] [Google Scholar]
- Uckun F. M., Ramakrishnan S., Houston L. L. Increased efficiency in selective elimination of leukemia cells by a combination of a stable derivative of cyclophosphamide and a human B-cell-specific immunotoxin containing pokeweed antiviral protein. Cancer Res. 1985 Jan;45(1):69–75. [PubMed] [Google Scholar]