Table 4. Histone H4 gene mutations and phenotypes of hst3Δ hst4Δ mutant cells.
| Histone mutant | Sensitivity at 37° | MMS sensitivity | HU sensitivity |
|---|---|---|---|
| hst3Δ hst4Δ | |||
| H4 WT | Ts- | S | S |
| H4 S1A | Ts- | S | S |
| H4 S1E | Ts- | S | S |
| H4 R3A | Ts- | S | S |
| H4 R3K | Ts- | S | S |
| H4 K5A | Ts- | S | S |
| H4 K5R | Ts- | S | S |
| H4 K5Q | Ts- | S | S |
| H4 K8A | Ts- | S | S |
| H4 K8R | Ts- | S | S |
| H4 K8Q | Ts- | S | S |
| H4 K12A | Ts- | S | S |
| H4 K12R | Ts- | S | S |
| H4 K12Q | Ts- | S | S |
| H4 K16R | Tr | R | S |
| H4 K16A | Tr | R | S |
| H4 K20A | Tr | R | S |
| H4 K20R | Ts- | S | S |
| H4 K20Q | Tr | R | S |
| H4 K31A | Ts- | S | S |
| H4 K31R | Ts- | S | S |
| H4 K31Q | Ts- | S | S |
| H4 S47A | Ts- | S | S |
| H4 S47E | Ts- | S | S |
| H4 K59A | Ts- | S | S |
| H4 K59R | Ts- | S | S |
| H4 K59Q | Ts- | S | S |
| H4 K77A | Ts- | S | S |
| H4 K77R | Ts- | S | S |
| H4 K77Q | Ts- | S | S |
| H4 K79A | Ts- | S | S |
| H4 K79R | Ts- | S | S |
| H4 K79Q | Ts- | S | S |
| H4 K91A | Ts- | S | S |
| H4 R91R | Ts- | S | S |
| H4 K91Q | Ts- | S | S |
| H4 R92A | Ts- | S | S |
| H4 R92K | Ts- | S | S |
Ts-, thermosensitive (fails to grow at 37°); Tr, thermoresistant (grows at 37°); S, growth compromised on plates containing either 0.01% MMS or 100 mM HU; R, histone gene mutations that rescue, at least partially, the MMS or HU sensitivity of hst3Δ hst4Δ cells.