Figure 5.
Western blot analysis of Dcw1-FLAG expression in S. cerevisiae. (Top) Anti-FLAG immunoblots of S. cerevisiae strains expressing Dcw1-FLAG alleles, representing the cytoplasmic, plasma membrane, and cell-wall fractions from each strain. Material from 7.5 × 105 cells is loaded for each strain in each fraction. Wells are labeled with the amino acid site of the FLAG tag within Dcw1. (Bottom) Anti-Pgk1 immunoblot of the cytoplasmic and plasma membrane fractions from strains carrying Dcw1-FLAG alleles. Cytoplasmic material from 3.75 × 105 cells is loaded for each strain. Membrane material from 3.5 × 105 cells was loaded for each strain