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. 1994 Jul 5;91(14):6525–6528. doi: 10.1073/pnas.91.14.6525

Positive selection of self- and alloreactive CD8+ T cells in Tap-1 mutant mice.

C J Aldrich 1, H G Ljunggren 1, L Van Kaer 1, P G Ashton-Rickardt 1, S Tonegawa 1, J Forman 1
PMCID: PMC44235  PMID: 8022816

Abstract

Mice with a homozygous deletion in their Tap-1 gene (-/- mice) express very low levels of cell membrane major histocompatibility complex class I molecules and have < 1% peripheral CD8+ T cells. We show that these -/- mice but not their +/- littermates display strong primary syngeneic anti-H-2Kb and -Db-specific responses mediated by CD8+ T cells. These responses are augmented by in vivo priming. Further, -/- mice primed in vivo with H-2d alloantigens generate an anti-H-2d response which appears nearly as strong as that found in +/- littermates. Both -/- anti-H-2b and anti-H-2d T cells do not recognize target cells from Tap-1 -/- animals or Tap-2-deficient RMA-S cells. Thus, some CD8+ anti-self and alloreactive T cells can be selected in the absence of Tap proteins.

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Selected References

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