Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1987 Nov;80(5):1232–1237. doi: 10.1172/JCI113197

Atrial natriuretic peptide protects against acute ischemic renal failure in the rat.

S G Shaw 1, P Weidmann 1, J Hodler 1, A Zimmermann 1, A Paternostro 1
PMCID: PMC442375  PMID: 2960693

Abstract

Because of its ability to increase glomerular filtration, antagonize the actions of vasoconstrictors, and produce vasodilation, alpha human atrial natriuretic peptide (alpha-hANP) was evaluated for its potentially beneficial effects in experimental ischemic renal failure induced by 45-60 min of renal artery occlusion in bilaterally or unilaterally renally intact Sprague-Dawley rats. After ischemia, a 4-h intrarenal infusion of alpha-hANP restored 14C-inulin clearances in bilaterally and unilaterally intact animals from 0.05 +/- 0.006 and 0.05 +/- 0.01 ml/min per 100 g to 0.314 +/- 0.04 and 0.25 +/- 0.01 ml/min per 100 g, respectively (P less than 0.001, n = 8), compared with normal values of 0.49 +/- 0.023 ml/min per 100 g. Histologically, there was a progressive decrease in medullary hyperemia and prevention of intratubular cell shedding and granulocyte margination as a result of the 4-h alpha-hANP infusion such that after 24 and 48 h the histological appearance of the tissue was essentially normal. The results show that a 4-h intrarenal infusion of alpha-hANP after renal ischemia can preserve glomerular filtration rate and reduce renal tissue damage.

Full text

PDF
1232

Images in this article

1234Image
on p.1234
1235Image
on p.1235
1235Image
on p.1235
1235Image
on p.1235
1235Image
on p.1235
1235Image
on p.1235
1235Image
on p.1235

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ackermann U. Structure and function of atrial natriuretic peptides. Clin Chem. 1986 Feb;32(2):241–247. [PubMed] [Google Scholar]
  2. Ballerman B. J., Brenner B. M. Biologically active atrial peptides. J Clin Invest. 1985 Dec;76(6):2041–2048. doi: 10.1172/JCI112206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ballermann B. J., Hoover R. L., Karnovsky M. J., Brenner B. M. Physiologic regulation of atrial natriuretic peptide receptors in rat renal glomeruli. J Clin Invest. 1985 Dec;76(6):2049–2056. doi: 10.1172/JCI112207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burke T. J., Schrier R. W. Ischemic acute renal failure--pathogenetic steps leading to acute tubular necrosis. Circ Shock. 1983;11(3):255–259. [PubMed] [Google Scholar]
  5. Debinski W., Kuchel O., Garcia R., Nguyen T. B., Racz K., Cantin M., Genest J. Atrial natriuretic factor inhibits the sympathetic nervous activity in one-kidney, one-clip hypertension in the rat. Proc Soc Exp Biol Med. 1986 Jan;181(1):173–177. doi: 10.3181/00379727-181-rc2. [DOI] [PubMed] [Google Scholar]
  6. Hirata Y., Tomita M., Yoshimi H., Ikeda M. Specific receptors for atrial natriuretic factor (ANF) in cultured vascular smooth muscle cells of rat aorta. Biochem Biophys Res Commun. 1984 Dec 14;125(2):562–568. doi: 10.1016/0006-291x(84)90576-x. [DOI] [PubMed] [Google Scholar]
  7. Kangawa K., Fukuda A., Kubota I., Hayashi Y., Matsuo H. Identification in rat atrial tissue of multiple forms of natriuretic polypeptides of about 3,000 daltons. Biochem Biophys Res Commun. 1984 Jun 15;121(2):585–591. doi: 10.1016/0006-291x(84)90222-5. [DOI] [PubMed] [Google Scholar]
  8. Maack T., Camargo M. J., Kleinert H. D., Laragh J. H., Atlas S. A. Atrial natriuretic factor: structure and functional properties. Kidney Int. 1985 Apr;27(4):607–615. doi: 10.1038/ki.1985.54. [DOI] [PubMed] [Google Scholar]
  9. Mason J., Torhorst J., Welsch J. Role of the medullary perfusion defect in the pathogenesis of ischemic renal failure. Kidney Int. 1984 Sep;26(3):283–293. doi: 10.1038/ki.1984.171. [DOI] [PubMed] [Google Scholar]
  10. Mason J., Welsch J., Takabatake T. Disparity between surface and deep nephron function early after renal ischemia. Kidney Int. 1983 Jul;24(1):27–36. doi: 10.1038/ki.1983.122. [DOI] [PubMed] [Google Scholar]
  11. Misono K. S., Grammer R. T., Fukumi H., Inagami T. Rat atrial natriuretic factor: isolation, structure and biological activities of four major peptides. Biochem Biophys Res Commun. 1984 Sep 17;123(2):444–451. doi: 10.1016/0006-291x(84)90250-x. [DOI] [PubMed] [Google Scholar]
  12. Needleman P., Adams S. P., Cole B. R., Currie M. G., Geller D. M., Michener M. L., Saper C. B., Schwartz D., Standaert D. G. Atriopeptins as cardiac hormones. Hypertension. 1985 Jul-Aug;7(4):469–482. doi: 10.1161/01.hyp.7.4.469. [DOI] [PubMed] [Google Scholar]
  13. Samson W. K., Vanatta J. C. Atrial natriuretic factor inhibits vasotocin-induced water reabsorption in the toad urinary bladder. Proc Soc Exp Biol Med. 1986 Jan;181(1):169–172. doi: 10.3181/00379727-181-rc1. [DOI] [PubMed] [Google Scholar]
  14. Shemesh O., Golbetz H., Kriss J. P., Myers B. D. Limitations of creatinine as a filtration marker in glomerulopathic patients. Kidney Int. 1985 Nov;28(5):830–838. doi: 10.1038/ki.1985.205. [DOI] [PubMed] [Google Scholar]
  15. Sonnenberg H., Cupples W. A., de Bold A. J., Veress A. T. Intrarenal localization of the natriuretic effect of cardiac atrial extract. Can J Physiol Pharmacol. 1982 Sep;60(9):1149–1152. doi: 10.1139/y82-166. [DOI] [PubMed] [Google Scholar]
  16. Stott R. B., Cameron J. S., Ogg C. S., Bewick M. Why the persistently high mortality in acute renal failure. Lancet. 1972 Jul 8;2(7767):75–79. doi: 10.1016/s0140-6736(72)91562-0. [DOI] [PubMed] [Google Scholar]
  17. Tanner G. A., Sloan K. L., Sophasan S. Effects of renal artery occlusion on kidney function in the rat. Kidney Int. 1973 Dec;4(6):377–389. doi: 10.1038/ki.1973.134. [DOI] [PubMed] [Google Scholar]
  18. Weidmann P., Hasler L., Gnädinger M. P., Lang R. E., Uehlinger D. E., Shaw S., Rascher W., Reubi F. C. Blood levels and renal effects of atrial natriuretic peptide in normal man. J Clin Invest. 1986 Mar;77(3):734–742. doi: 10.1172/JCI112368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yared A., Kon V., Ichikawa I. Mechanism of preservation of glomerular perfusion and filtration during acute extracellular fluid volume depletion. Importance of intrarenal vasopressin-prostaglandin interaction for protecting kidneys from constrictor action of vasopressin. J Clin Invest. 1985 May;75(5):1477–1487. doi: 10.1172/JCI111851. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES