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. 1987 Nov;80(5):1255–1260. doi: 10.1172/JCI113200

Osmoprotective activity for Escherichia coli in mammalian renal inner medulla and urine. Correlation of glycine and proline betaines and sorbitol with response to osmotic loads.

S T Chambers 1, C M Kunin 1
PMCID: PMC442378  PMID: 3316273

Abstract

Escherichia coli are protected against hypertonic NaCl by human urine. We have shown that this is due in part to the presence of glycine betaine and proline betaine. Several investigators have proposed that betaines and sorbitol are concentrated in the cells of the renal inner medulla where they exert a protective role against urea and extracellular osmotic forces. E. coli was used in the present studies as an "osmosensor" to detect osmoprotective activity in mammalian tissues. The greatest activity was found in extracts of renal inner medulla and to a lesser extent in the renal outer medulla and cortex of several mammalian species. Liver extracts were more active than other nonrenal tissues. Bacterial osmoprotective activity and concentration of glycine betaine in the renal inner medulla of rabbits were found to correlate closely with urinary osmolarity. Concentrations of sorbitol were found to be also increased in the renal inner medulla during osmotic stress, but this compound is not osmoprotective for E. coli. Glycine and proline betaine were recovered in urine of rabbits and were increased in those given high osmotic loads. Only small amounts of proline betaine were recovered in the renal inner medulla. The source from which proline betaine is derived is unknown.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEESON P. B., ROCHA H., GUZE L. B. Experimental pyelonephritis: influence of localized injury in different parts of the kidney on susceptibility to hematogenous infection. Trans Assoc Am Physicians. 1957;70:120–126. [PubMed] [Google Scholar]
  2. Bagnasco S., Balaban R., Fales H. M., Yang Y. M., Burg M. Predominant osmotically active organic solutes in rat and rabbit renal medullas. J Biol Chem. 1986 May 5;261(13):5872–5877. [PubMed] [Google Scholar]
  3. Balaban R. S., Knepper M. A. Nitrogen-14 nuclear magnetic resonance spectroscopy of mammalian tissues. Am J Physiol. 1983 Nov;245(5 Pt 1):C439–C444. doi: 10.1152/ajpcell.1983.245.5.C439. [DOI] [PubMed] [Google Scholar]
  4. Barak A. J., Tuma D. J. A simplified procedure for the determination of betaine in liver. Lipids. 1979 Oct;14(10):860–863. doi: 10.1007/BF02534129. [DOI] [PubMed] [Google Scholar]
  5. Beck F., Dörge A., Rick R., Thurau K. Intra- and extracellular element concentrations of rat renal papilla in antidiuresis. Kidney Int. 1984 Feb;25(2):397–403. doi: 10.1038/ki.1984.30. [DOI] [PubMed] [Google Scholar]
  6. Chambers S. T., Kunin C. M. Isolation of glycine betaine and proline betaine from human urine. Assessment of their role as osmoprotective agents for bacteria and the kidney. J Clin Invest. 1987 Mar;79(3):731–737. doi: 10.1172/JCI112878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chambers S., Kunin C. M. The osmoprotective properties of urine for bacteria: the protective effect of betaine and human urine against low pH and high concentrations of electrolytes, sugars, and urea. J Infect Dis. 1985 Dec;152(6):1308–1316. doi: 10.1093/infdis/152.6.1308. [DOI] [PubMed] [Google Scholar]
  8. Imhoff J. F., Rodriguez-Valera F. Betaine is the main compatible solute of halophilic eubacteria. J Bacteriol. 1984 Oct;160(1):478–479. doi: 10.1128/jb.160.1.478-479.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LALICH J. J., KLINE B. E., RUSCH H. P. Degenerative renal lesions induced by prolonged choline deficiency. AMA Arch Pathol. 1949 Dec;48(6):583-92, illust. [PubMed] [Google Scholar]
  10. Le Rudulier D., Strom A. R., Dandekar A. M., Smith L. T., Valentine R. C. Molecular biology of osmoregulation. Science. 1984 Jun 8;224(4653):1064–1068. doi: 10.1126/science.224.4653.1064. [DOI] [PubMed] [Google Scholar]
  11. Martin J. J., Finkelstein J. D. Enzymatic determination of betaine in rat tissues. Anal Biochem. 1981 Feb;111(1):72–76. doi: 10.1016/0003-2697(81)90230-x. [DOI] [PubMed] [Google Scholar]
  12. Perroud B., Le Rudulier D. Glycine betaine transport in Escherichia coli: osmotic modulation. J Bacteriol. 1985 Jan;161(1):393–401. doi: 10.1128/jb.161.1.393-401.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rennick B. R. Renal tubule transport of organic cations. Am J Physiol. 1981 Feb;240(2):F83–F89. doi: 10.1152/ajprenal.1981.240.2.F83. [DOI] [PubMed] [Google Scholar]
  14. Yancey P. H., Clark M. E., Hand S. C., Bowlus R. D., Somero G. N. Living with water stress: evolution of osmolyte systems. Science. 1982 Sep 24;217(4566):1214–1222. doi: 10.1126/science.7112124. [DOI] [PubMed] [Google Scholar]

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