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. Author manuscript; available in PMC: 2015 Aug 13.
Published in final edited form as: Science. 2015 Feb 13;347(6223):779–784. doi: 10.1126/science.aaa0314

Fig. 3. Activity of AI-10-49 in inv(16)mousemodel and inv(16) AML patient samples.

Fig. 3

(A) Kaplan-Meier survival curve of mice (n = 11 per group) transplanted with 2 × 103 cbfb+/MYH11;Ras+/G12D leukemic cells and treated between days 5 and 15 posttransplantation (blue arrows)with DMSO(black line) or 200 mg/kg of body weight per day AI-10-49 (red line); Statistics described in the statistical methods section. (B) Percent viability (annexin V and 7AAD assay) relative to vehicle control (DMSO) for CD34+ purified primary human inv(16) AML samples treated for 48 hours with either AI-10-49 or AI-10-47 at the indicated concentrations. Each symbol represents the average for an individual sample from duplicate treatments. The line represents the mean; error bars represent the SD. (C) Percent viability (annexin V and 7AAD assay) relative to vehicle control (DMSO) for primary human AML samples with normal karyotype treated for 48 hours with either AI-10-49 or AI-10-47 at the indicated concentrations. Each symbol represents the average for an individual sample from duplicate treatments.The line represents the mean of all biological replicates; error bars represent the SD. (D) Percentage of colony-forming units (CFUs) after treatment with AI-10-49 relative to vehicle control (DMSO) for primary human AML cells. Each symbol represents the average for an individual sample from duplicate treatments; error bars represent the SD. (E) Percent CFUs to vehicle control (DMSO) for CD34+ purified primary human inv(16) AML samples treated with either AI-10-49 or AI-10-47 at the indicated concentrations. CFU assays were performed in triplicate. Error bars represent the SD. (F) Percent CFUs to vehicle control (DMSO) for CD34+ purified primary human AML samples with normal karyotype treated with either AI-10-49 or AI-10-47 at the indicated concentrations. CFU assays were performed in triplicate. Error bars represent the SD. (G) Percent CFUs to vehicle control (DMSO) for CD34+ purified primary CD34+ cord blood cells treated with either AI-10-49 or AI-10-47 at the indicated concentrations. Significance calculated as unpaired t test, *P < 0.05 or ***P < 0.001.