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. 1987 Nov;80(5):1455–1458. doi: 10.1172/JCI113225

Selective binding of somatostatin-14 and somatostatin-28 to islet cells revealed by quantitative electron microscopic autoradiography.

M Amherdt 1, Y C Patel 1, L Orci 1
PMCID: PMC442403  PMID: 2890658

Abstract

Quantitative electron microscopic autoradiography was used for comparing the binding of labeled somatostatin-14 (S-14) and somatostatin-28 (S-28 section) to islet cells. Monolayer cultures of rat islet cells were incubated with [125I-Tyr11]S-14 (S-14 section) or [125I-Leu8, D-Trp22, Tyr25]S-28 (S-28 section) in the presence or absence of excess unlabeled peptides. Autoradiographic grains (ARG) associated with individual islet cells were identified and expressed as the mean number per B, A, and D cells. Specific ARG associated with S-14 were found over B and A cells. S-28 section-related specific ARG were concentrated over B, A, as well as D cells. The highest density of S-14 section labeling occurred over A cells, which under conditions of maximum labeling (37 degrees C for 60 min) contained five times as many ARG as did B cells. By contrast, under the same incubation conditions, the labeling density with S-28 section was maximal over B cells, which contained four and five times as many grains as A and D cells, respectively. These observations show preferential association of S-14 section with the A cell and S-28 section with the B cel provide strong evidence for the existence of separate binding sites for S-14 section and S-28 section on A and B cells, respectively, which presumably mediate the previously reported glucagon selective inhibitory effect of S-14 and the insulin-selective action of S-28.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benoit R., Böhlen P., Brazeau P., Ling N., Guillemin R. Isolation and characterization of rat pancreatic somatostatin. Endocrinology. 1980 Dec;107(6):2127–2129. doi: 10.1210/endo-107-6-2127. [DOI] [PubMed] [Google Scholar]
  2. Bonner-Weir S., Orci L. New perspectives on the microvasculature of the islets of Langerhans in the rat. Diabetes. 1982 Oct;31(10):883–889. doi: 10.2337/diab.31.10.883. [DOI] [PubMed] [Google Scholar]
  3. CARO L. G., VAN TUBERGEN R. P., KOLB J. A. High-resolution autoradiography. I. Methods. J Cell Biol. 1962 Nov;15:173–188. doi: 10.1083/jcb.15.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Funckes C. L., Minth C. D., Deschenes R., Magazin M., Tavianini M. A., Sheets M., Collier K., Weith H. L., Aron D. C., Roos B. A. Cloning and characterization of a mRNA-encoding rat preprosomatostatin. J Biol Chem. 1983 Jul 25;258(14):8781–8787. [PubMed] [Google Scholar]
  5. Goodman R. H., Aron D. C., Roos B. A. Rat pre-prosomatostatin. Structure and processing by microsomal membranes. J Biol Chem. 1983 May 10;258(9):5570–5573. [PubMed] [Google Scholar]
  6. Ipp E., Rivier J., Dobbs R. E., Brown M., Vale W., Unger R. H. Somatostatin analogs inhibit somatostatin release. Endocrinology. 1979 May;104(5):1270–1273. doi: 10.1210/endo-104-5-1270. [DOI] [PubMed] [Google Scholar]
  7. Leroux P., Quirion R., Pelletier G. Localization and characterization of brain somatostatin receptors as studied with somatostatin-14 and somatostatin-28 receptor radioautography. Brain Res. 1985 Nov 11;347(1):74–84. doi: 10.1016/0006-8993(85)90890-x. [DOI] [PubMed] [Google Scholar]
  8. Mandarino L., Stenner D., Blanchard W., Nissen S., Gerich J., Ling N., Brazeau P., Bohlen P., Esch F., Guillemin R. Selective effects of somatostatin-14, -25 and -28 on in vitro insulin and glucagon secretion. Nature. 1981 May 7;291(5810):76–77. doi: 10.1038/291076a0. [DOI] [PubMed] [Google Scholar]
  9. Montesano R., Mouron P., Amherdt M., Orci L. Collagen matrix promotes reorganization of pancreatic endocrine cell monolayers into islet-like organoids. J Cell Biol. 1983 Sep;97(3):935–939. doi: 10.1083/jcb.97.3.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Montminy M. R., Goodman R. H., Horovitch S. J., Habener J. F. Primary structure of the gene encoding rat preprosomatostatin. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3337–3340. doi: 10.1073/pnas.81.11.3337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Noe B. D., Debo G., Spiess J. Comparison of prohormone-processing activities in islet microsomes and secretory granules: evidence for distinct converting enzymes for separate islet prosomatostatins. J Cell Biol. 1984 Aug;99(2):578–587. doi: 10.1083/jcb.99.2.578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Orci L., Unger R. H. Functional subdivision of islets of Langerhans and possible role of D cells. Lancet. 1975 Dec 20;2(7947):1243–1244. doi: 10.1016/s0140-6736(75)92078-4. [DOI] [PubMed] [Google Scholar]
  13. Patel Y. C., Amherdt M., Orci L. Quantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets. Science. 1982 Sep 17;217(4565):1155–1156. doi: 10.1126/science.6126003. [DOI] [PubMed] [Google Scholar]
  14. Patel Y. C., Baquiran G., Srikant C. B., Posner B. I. Quantitative in vivo autoradiographic localization of [125I-Tyr11]somatostatin-14- and [Leu8,D-Trp22-125I-Tyr25]somatostatin-28-binding sites in rat brain. Endocrinology. 1986 Nov;119(5):2262–2269. doi: 10.1210/endo-119-5-2262. [DOI] [PubMed] [Google Scholar]
  15. Patel Y. C., Wheatley T., Ning C. Multiple forms of immunoreactive somatostatin: comparison of distribution in neural and nonneural tissues and portal plasma of the rat. Endocrinology. 1981 Dec;109(6):1943–1949. doi: 10.1210/endo-109-6-1943. [DOI] [PubMed] [Google Scholar]
  16. Peterfreund R. A., Vale W. W. Somatostatin analogs inhibit somatostatin secretion from cultured hypothalamus cells. Neuroendocrinology. 1984 Nov;39(5):397–402. doi: 10.1159/000124011. [DOI] [PubMed] [Google Scholar]
  17. Polonsky K. S., Shoelson S. E., Docherty H. M. Plasma somatostatin 28 increases in response to feeding in man. J Clin Invest. 1983 May;71(5):1514–1518. doi: 10.1172/JCI110907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ravazzola M., Benoit R., Ling N., Guillemin R., Orci L. Immunocytochemical localization of prosomatostatin fragments in maturing and mature secretory granules of pancreatic and gastrointestinal D cells. Proc Natl Acad Sci U S A. 1983 Jan;80(1):215–218. doi: 10.1073/pnas.80.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Srikant C. B., Patel Y. C. Receptor binding of somatostatin-28 is tissue specific. Nature. 1981 Nov 19;294(5838):259–260. doi: 10.1038/294259a0. [DOI] [PubMed] [Google Scholar]
  20. Srikant C. B., Patel Y. C. Somatostatin receptors. Adv Exp Med Biol. 1985;188:291–304. doi: 10.1007/978-1-4615-7886-4_16. [DOI] [PubMed] [Google Scholar]
  21. Tavianini M. A., Hayes T. E., Magazin M. D., Minth C. D., Dixon J. E. Isolation, characterization, and DNA sequence of the rat somatostatin gene. J Biol Chem. 1984 Oct 10;259(19):11798–11803. [PubMed] [Google Scholar]

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