Figure 1.

Secondary Structure Prediction and Comparison with the Crystal Structure of sFlaF

The full-length FlaF secondary structure was predicted by the Phyre 2 server. N-terminal residues 3–42 were predicted to be present as a long helical region. The actual secondary structure obtained from the crystal structure of sFlaF chain B (PDB, 4P94) is shown for comparison below the prediction. The sequence was color coded from cyan (most variable) to dark purple (most conserved) as conservation shown in Figure 6A.