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. 1988 May;81(5):1427–1433. doi: 10.1172/JCI113473

Requirements for the construction of antibody heterodimers for the direction of lysis of tumors by human T cells.

C F Scott Jr 1, W A Blättler 1, J M Lambert 1, R S Kalish 1, C Morimoto 1, S F Schlossman 1
PMCID: PMC442574  PMID: 2966815

Abstract

We constructed a series of MAb heterodimers consisting of the J5 (anti-common acute lymphoblastic leukemia antigen [CALLA]) antibody and antibodies to a variety of structures present on the surface of activated human T cells, including CD3 antigen (T cell receptor-associated glycoproteins), CD2 antigen (T11/E-rosette receptor), CD25 antigen (IL-2 receptor), and the transferrin receptor. We tested the ability of these heterodimers to direct a CD2 + CD3 + CD8 + CD4 - CD25 + transferrin receptor + MHC-restricted human cytolytic T lymphocyte (CTL) clone to lyse a CALLA + human tumor in vitro. Only heterodimers containing an anti-CD3 antibody or activating antibodies to CD2 could direct the clone to lyse these human tumor targets, even when the clone was additionally activated with anti-CD3 or anti-CD2 antibodies. Our findings may have implications in the design of strategies for the use of such reagents in the treatment of human neoplasia.

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Selected References

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