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. 1988 May;81(5):1646–1650. doi: 10.1172/JCI113500

Proteolytic inactivation of alpha-1-proteinase inhibitor by a neutrophil metalloproteinase.

P E Desrochers 1, S J Weiss 1
PMCID: PMC442601  PMID: 3259248

Abstract

Human neutrophils triggered with phorbol myristate acetate or opsonized zymosan particles released a metalloproteinase (MP) capable of cleaving and inactivating alpha-1-proteinase inhibitor (alpha-1-PI). Sequence analysis of the amino acids in proteolyzed, native alpha-1-PI revealed a unique single cleavage site between Phe-352 and Leu-353. An analysis of the process regulating the enzyme's activity revealed that the neutrophil MP was released from cells in a latent form whose activation was tightly linked to the generation of hypochlorous acid. These results indicate that human neutrophils use chlorinated oxidants to activate a latent MP that is capable of proteolytically inactivating alpha-1-PI by cleaving the antiproteinase at a unique point in its inhibitory site region.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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