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. 1988 Oct;82(4):1282–1287. doi: 10.1172/JCI113727

Human recombinant granulocyte-macrophage colony stimulating factor and interleukin 3 have overlapping but distinct hematopoietic activities.

S G Emerson 1, Y C Yang 1, S C Clark 1, M W Long 1
PMCID: PMC442680  PMID: 3049674

Abstract

The hematopoietic stimulatory activities of human recombinant IL-3 and granulocyte-macrophage colony stimulating factor (GM-CSF) were directly compared using highly enriched human bone marrow progenitor target cells. IL-3 supported a larger number of erythroid and megakaryocytic progenitor cells than did GM-CSF, while GM-CSF supported more myeloid progenitors. IL-3 directly stimulated the division and migration of primitive erythroid burst forming units, while GM-CSF merely sustained their net survival in culture without promoting division and expansion. IL-3 promoted the formation of larger numbers of multipotential granulocyte-erythroid-macrophage-megakaryocyte colony forming unit--derived colonies than did GM-CSF. These data indicate that human IL-3 and GM-CSF have overlapping but distinct hematopoietic activities, and suggest a potential role for the clinical application of combined IL-3/GM-CSF therapy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beverley P. C., Linch D., Delia D. Isolation of human haematopoietic progenitor cells using monoclonal antibodies. Nature. 1980 Sep 25;287(5780):332–333. doi: 10.1038/287332a0. [DOI] [PubMed] [Google Scholar]
  2. Donahue R. E., Emerson S. G., Wang E. A., Wong G. G., Clark S. C., Nathan D. G. Demonstration of burst-promoting activity of recombinant human GM-CSF on circulating erythroid progenitors using an assay involving the delayed addition of erythropoietin. Blood. 1985 Dec;66(6):1479–1481. [PubMed] [Google Scholar]
  3. Emerson S. G., Sieff C. A., Wang E. A., Wong G. G., Clark S. C., Nathan D. G. Purification of fetal hematopoietic progenitors and demonstration of recombinant multipotential colony-stimulating activity. J Clin Invest. 1985 Sep;76(3):1286–1290. doi: 10.1172/JCI112087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Golde D. W., Bersch N., Quan S. G., Lusis A. J. Production of erythroid-potentiating activity by a human T-lymphoblast cell line. Proc Natl Acad Sci U S A. 1980 Jan;77(1):593–596. doi: 10.1073/pnas.77.1.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jacobs K., Shoemaker C., Rudersdorf R., Neill S. D., Kaufman R. J., Mufson A., Seehra J., Jones S. S., Hewick R., Fritsch E. F. Isolation and characterization of genomic and cDNA clones of human erythropoietin. 1985 Feb 28-Mar 6Nature. 313(6005):806–810. doi: 10.1038/313806a0. [DOI] [PubMed] [Google Scholar]
  6. Kimura H., Burstein S. A., Thorning D., Powell J. S., Harker L. A., Fialkow P. J., Adamson J. W. Human megakaryocytic progenitors (CFU-M) assayed in methylcellulose: physical characteristics and requirements for growth. J Cell Physiol. 1984 Jan;118(1):87–96. doi: 10.1002/jcp.1041180115. [DOI] [PubMed] [Google Scholar]
  7. Long M. W., Gragowski L. L., Heffner C. H., Boxer L. A. Phorbol diesters stimulate the development of an early murine progenitor cell. The burst-forming unit-megakaryocyte. J Clin Invest. 1985 Aug;76(2):431–438. doi: 10.1172/JCI111990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Long M. W., Smolen J. E., Szczepanski P., Boxer L. A. Role of phorbol diesters in in vitro murine megakaryocyte colony formation. J Clin Invest. 1984 Nov;74(5):1686–1692. doi: 10.1172/JCI111585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Messner H. A., Jamal N., Izaguirre C. The growth of large megakaryocyte colonies from human bone marrow. J Cell Physiol Suppl. 1982;1:45–51. doi: 10.1002/jcp.1041130410. [DOI] [PubMed] [Google Scholar]
  10. Metcalf D., Johnson G. R., Burgess A. W. Direct stimulation by purified GM-CSF of the proliferation of multipotential and erythroid precursor cells. Blood. 1980 Jan;55(1):138–147. [PubMed] [Google Scholar]
  11. Metcalf D., Johnson G. R. Interactions between purified GM-CSF, purified erythropoietin and spleen conditioned medium on hemopoietic colony formation in vitro. J Cell Physiol. 1979 May;99(2):159–174. doi: 10.1002/jcp.1040990202. [DOI] [PubMed] [Google Scholar]
  12. Myers C. D., Katz F. E., Joshi G., Millar J. L. A cell line secreting stimulating factors for CFU-GEMM culture. Blood. 1984 Jul;64(1):152–155. [PubMed] [Google Scholar]
  13. Robinson B. E., McGrath H. E., Quesenberry P. J. Recombinant murine granulocyte macrophage colony-stimulating factor has megakaryocyte colony-stimulating activity and augments megakaryocyte colony stimulation by interleukin 3. J Clin Invest. 1987 Jun;79(6):1648–1652. doi: 10.1172/JCI113002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sieff C. A., Emerson S. G., Donahue R. E., Nathan D. G., Wang E. A., Wong G. G., Clark S. C. Human recombinant granulocyte-macrophage colony-stimulating factor: a multilineage hematopoietin. Science. 1985 Dec 6;230(4730):1171–1173. doi: 10.1126/science.3877981. [DOI] [PubMed] [Google Scholar]
  15. Solberg L. A., Jr, Jamal N., Messner H. A. Characterization of human megakaryocytic colony formation in human plasma. J Cell Physiol. 1985 Jul;124(1):67–74. doi: 10.1002/jcp.1041240112. [DOI] [PubMed] [Google Scholar]
  16. Strauss L. C., Brovall C., Fackler M. J., Schwartz J. F., Shaper J. H., Loken M. R., Civin C. I. Antigenic analysis of hematopoiesis. IV. The My-11 hematopoietic cell surface antigen is expressed by myelomonocytic and lymphoid, but not erythroid, progenitor cells. Exp Hematol. 1986 Nov;14(10):935–945. [PubMed] [Google Scholar]
  17. Tayrien G., Rosenberg R. D. Purification and properties of a megakaryocyte stimulatory factor present both in the serum-free conditioned medium of human embryonic kidney cells and in thrombocytopenic plasma. J Biol Chem. 1987 Mar 5;262(7):3262–3268. [PubMed] [Google Scholar]
  18. Welte K., Platzer E., Lu L., Gabrilove J. L., Levi E., Mertelsmann R., Moore M. A. Purification and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1526–1530. doi: 10.1073/pnas.82.5.1526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wong G. G., Temple P. A., Leary A. C., Witek-Giannotti J. S., Yang Y. C., Ciarletta A. B., Chung M., Murtha P., Kriz R., Kaufman R. J. Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human urinary protein. Science. 1987 Mar 20;235(4795):1504–1508. doi: 10.1126/science.3493529. [DOI] [PubMed] [Google Scholar]
  20. Wong G. G., Witek J. S., Temple P. A., Wilkens K. M., Leary A. C., Luxenberg D. P., Jones S. S., Brown E. L., Kay R. M., Orr E. C. Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science. 1985 May 17;228(4701):810–815. doi: 10.1126/science.3923623. [DOI] [PubMed] [Google Scholar]
  21. Yang Y. C., Ciarletta A. B., Temple P. A., Chung M. P., Kovacic S., Witek-Giannotti J. S., Leary A. C., Kriz R., Donahue R. E., Wong G. G. Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3. Cell. 1986 Oct 10;47(1):3–10. doi: 10.1016/0092-8674(86)90360-0. [DOI] [PubMed] [Google Scholar]

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