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. 2015 May 7;145(2):258–267. doi: 10.1111/imm.12442

Figure 5.

Figure 5

The role of cathepsin B in enterohaemorrhagic Escherichia coli (EHEC) O157:H7 enterohaemolysin-induced interleukin-1β (IL-1β) production by human THP-1 cells and mouse IC-21 cells. Cells were left untreated or were infected with EDL933, ΔehxA or ΔehxA/pehxA for 2 hr. Cathepsin B was visualized by confocal laser scanning microscopy after staining with a specific antibody. Actin was stained with phalloidin AF488. (a) Cathepsin B in human THP-1 cells. (b) Cathepsin B in mouse IC-21 cells. (c) The THP-1 cells were treated with 10 μm of the cathepsin B inhibitor CA-074Me for 1 hr before infection with EHEC O157:H7. The IL-1β concentration in the supernatant was determined 4 hr post-infection by ELISA. (c) The results represent the mean ± SD of three independent experiments. Significant differences (*P < 0·05) are indicated. n.s., not significant (P > 0·05).