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. 1988 Dec;82(6):1871–1876. doi: 10.1172/JCI113804

Growth hormone enhances hepatic epidermal growth factor receptor concentration in mice.

J O Jansson 1, S Ekberg 1, S B Hoath 1, W G Beamer 1, L A Frohman 1
PMCID: PMC442766  PMID: 3264288

Abstract

The effect of growth hormone (GH) on binding of epidermal growth factor (EGF) to liver membrane preparations was investigated in hypophysectomized mice and partially GH-deficient, genetic mutant "little" (lit/lit) mice. The EGF binding of normal male mice and testosterone-treated females was higher than in normal females. Due to diminished receptor concentration, hepatic EGF binding was decreased in male and female lit/lit mice to a level that was unaffected by gender or androgen treatment. GH replacement therapy by intermittent injections and continuous infusion restored the EGF binding of hypophysectomized mice to normal male and female levels, respectively, suggesting a role for the more pulsatile GH secretion in normal males. In lit/lit mice, however, both continuous and intermittent GH resulted in EGF binding levels comparable to those in normal females. In normal males continuous GH suppressed EGF binding. In conclusion, endogenous GH secretion induces EGF receptors in mice and this effect may be modulated by sex differences in GH secretion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alm J., Lakshmanan J., Fisher D. A. Sexual dimorphism and testosterone effects on liver epidermal growth factor receptors in mice. Acta Endocrinol (Copenh) 1986 Sep;113(1):140–144. doi: 10.1530/acta.0.1130140. [DOI] [PubMed] [Google Scholar]
  2. Balk S. D., Shiu R. P., LaFleur M. M., Young L. L. Epidermal growth factor and insulin cause normal chicken heart mesenchymal cells to proliferate like their Rous sarcoma virus-infected counterparts. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1154–1157. doi: 10.1073/pnas.79.4.1154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benveniste R., Carson S. A. Binding characteristics of epidermal growth factor receptors in male and female rat liver cell membrane preparations. Mol Cell Endocrinol. 1985 Jul;41(2-3):147–151. doi: 10.1016/0303-7207(85)90017-6. [DOI] [PubMed] [Google Scholar]
  4. Carpenter G. Receptors for epidermal growth factor and other polypeptide mitogens. Annu Rev Biochem. 1987;56:881–914. doi: 10.1146/annurev.bi.56.070187.004313. [DOI] [PubMed] [Google Scholar]
  5. Carr F. E., Seelig S., Mariash C. N., Schwartz H. L., Oppenheimer J. H. Starvation and hypothyroidism exert an overlapping influence on rat hepatic messenger RNA activity profiles. J Clin Invest. 1983 Jul;72(1):154–163. doi: 10.1172/JCI110953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chubb C., Nolan C. Animal models of male infertility: mice bearing single-gene mutations that induce infertility. Endocrinology. 1985 Jul;117(1):338–346. doi: 10.1210/endo-117-1-338. [DOI] [PubMed] [Google Scholar]
  7. Clark R. G., Jansson J. O., Isaksson O., Robinson I. C. Intravenous growth hormone: growth responses to patterned infusions in hypophysectomized rats. J Endocrinol. 1985 Jan;104(1):53–61. doi: 10.1677/joe.0.1040053. [DOI] [PubMed] [Google Scholar]
  8. Cohen S., Taylor J. M. Epidermal growth factor: chemical and biological characterization. Recent Prog Horm Res. 1974;30(0):533–550. doi: 10.1016/b978-0-12-571130-2.50017-1. [DOI] [PubMed] [Google Scholar]
  9. Cuatrecasas P. Affinity chromatography and purification of the insulin receptor of liver cell membranes. Proc Natl Acad Sci U S A. 1972 May;69(5):1277–1281. doi: 10.1073/pnas.69.5.1277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. D'Ercole A. J., Stiles A. D., Underwood L. E. Tissue concentrations of somatomedin C: further evidence for multiple sites of synthesis and paracrine or autocrine mechanisms of action. Proc Natl Acad Sci U S A. 1984 Feb;81(3):935–939. doi: 10.1073/pnas.81.3.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Di Fiore P. P., Pierce J. H., Fleming T. P., Hazan R., Ullrich A., King C. R., Schlessinger J., Aaronson S. A. Overexpression of the human EGF receptor confers an EGF-dependent transformed phenotype to NIH 3T3 cells. Cell. 1987 Dec 24;51(6):1063–1070. doi: 10.1016/0092-8674(87)90592-7. [DOI] [PubMed] [Google Scholar]
  12. Eicher E. M., Beamer W. G. Inherited ateliotic dwarfism in mice. Characteristics of the mutation, little, on chromosome 6. J Hered. 1976 Mar-Apr;67(2):87–91. doi: 10.1093/oxfordjournals.jhered.a108682. [DOI] [PubMed] [Google Scholar]
  13. Froesch E. R., Schmid C., Schwander J., Zapf J. Actions of insulin-like growth factors. Annu Rev Physiol. 1985;47:443–467. doi: 10.1146/annurev.ph.47.030185.002303. [DOI] [PubMed] [Google Scholar]
  14. Gustafsson J. A., Mode A., Norstedt G., Skett P. Sex steroid induced changes in hepatic enzymes. Annu Rev Physiol. 1983;45:51–60. doi: 10.1146/annurev.ph.45.030183.000411. [DOI] [PubMed] [Google Scholar]
  15. Herington A. C., Harrison D., Graystone J. Hepatic binding of human and bovine growth hormones and ovine prolactin in the dwarf "little" mouse. Endocrinology. 1983 Jun;112(6):2032–2038. doi: 10.1210/endo-112-6-2032. [DOI] [PubMed] [Google Scholar]
  16. Hoath S. B., Pickens W. L., Bucuvalas J. C., Suchy F. J. Characterization of hepatic epidermal growth factor receptors in the developing rat. Biochim Biophys Acta. 1987 Aug 19;930(1):107–113. doi: 10.1016/0167-4889(87)90162-5. [DOI] [PubMed] [Google Scholar]
  17. Hock R. A., Hollenberg M. D. Characterization of the receptor for epidermal growth factor-urogastrone in human placenta membranes. J Biol Chem. 1980 Nov 25;255(22):10731–10736. [PubMed] [Google Scholar]
  18. Husman B., Norstedt G., Mode A., Gustafsson J. A. The mode of growth hormone administration is of major importance for the excretion of the major male rat urinary proteins. Mol Cell Endocrinol. 1985 May;40(2-3):205–210. doi: 10.1016/0303-7207(85)90176-5. [DOI] [PubMed] [Google Scholar]
  19. Isaksson O. G., Jansson J. O., Gause I. A. Growth hormone stimulates longitudinal bone growth directly. Science. 1982 Jun 11;216(4551):1237–1239. doi: 10.1126/science.7079756. [DOI] [PubMed] [Google Scholar]
  20. Jansson J. O., Albertsson-Wikland K., Edén S., Thorngren K. G., Isaksson O. Effect of frequency of growth hormone administration on longitudinal bone growth and body weight in hypophysectomized rats. Acta Physiol Scand. 1982 Feb;114(2):261–265. doi: 10.1111/j.1748-1716.1982.tb06980.x. [DOI] [PubMed] [Google Scholar]
  21. Jansson J. O., Downs T. R., Beamer W. G., Frohman L. A. Receptor-associated resistance to growth hormone-releasing factor in dwarf "little" mice. Science. 1986 Apr 25;232(4749):511–512. doi: 10.1126/science.3008329. [DOI] [PubMed] [Google Scholar]
  22. Jansson J. O., Edén S., Isaksson O. Sexual dimorphism in the control of growth hormone secretion. Endocr Rev. 1985 Spring;6(2):128–150. doi: 10.1210/edrv-6-2-128. [DOI] [PubMed] [Google Scholar]
  23. Jansson J. O., Frohman L. A. Differential effects of neonatal and adult androgen exposure on the growth hormone secretory pattern in male rats. Endocrinology. 1987 Apr;120(4):1551–1557. doi: 10.1210/endo-120-4-1551. [DOI] [PubMed] [Google Scholar]
  24. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  25. Mode A., Gustafsson J. A., Jansson J. O., Edén S., Isaksson O. Association between plasma level of growth hormone and sex differentiation of hepatic steroid metabolism in the rat. Endocrinology. 1982 Nov;111(5):1692–1697. doi: 10.1210/endo-111-5-1692. [DOI] [PubMed] [Google Scholar]
  26. Morgan E. T., MacGeoch C., Gustafsson J. A. Hormonal and developmental regulation of expression of the hepatic microsomal steroid 16 alpha-hydroxylase cytochrome P-450 apoprotein in the rat. J Biol Chem. 1985 Oct 5;260(22):11895–11898. [PubMed] [Google Scholar]
  27. Mukku V. R. Regulation of epidermal growth factor receptor levels by thyroid hormone. J Biol Chem. 1984 May 25;259(10):6543–6547. [PubMed] [Google Scholar]
  28. Mukku V. R., Stancel G. M. Regulation of epidermal growth factor receptor by estrogen. J Biol Chem. 1985 Aug 15;260(17):9820–9824. [PubMed] [Google Scholar]
  29. Norstedt G., Palmiter R. Secretory rhythm of growth hormone regulates sexual differentiation of mouse liver. Cell. 1984 Apr;36(4):805–812. doi: 10.1016/0092-8674(84)90030-8. [DOI] [PubMed] [Google Scholar]
  30. Noshiro M., Negishi M. Pretranslational regulation of sex-dependent testosterone hydroxylases by growth hormone in mouse liver. J Biol Chem. 1986 Dec 5;261(34):15923–15927. [PubMed] [Google Scholar]
  31. Phillips J. A., 3rd, Beamer W. G., Bartke A. Analysis of growth hormone genes in mice with genetic defects of growth hormone expression. J Endocrinol. 1982 Mar;92(3):405–407. doi: 10.1677/joe.0.0920405. [DOI] [PubMed] [Google Scholar]
  32. Richman R. A., Claus T. H., Pilkis S. J., Friedman D. L. Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3589–3593. doi: 10.1073/pnas.73.10.3589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Russell W. E., Van Wyk J. J., Pledger W. J. Inhibition of the mitogenic effects of plasma by a monoclonal antibody to somatomedin C. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2389–2392. doi: 10.1073/pnas.81.8.2389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Savage C. R., Jr, Inagami T., Cohen S. The primary structure of epidermal growth factor. J Biol Chem. 1972 Dec 10;247(23):7612–7621. [PubMed] [Google Scholar]
  35. Sinha Y. N., Salocks C. B., Wickes M. A., Vanderlaan W. P. Serum and pituitary concentrations of prolactin and growth hormone in mice during a twenty-four hour period. Endocrinology. 1977 Mar;100(3):786–791. doi: 10.1210/endo-100-3-786. [DOI] [PubMed] [Google Scholar]
  36. St Hilaire R. J., Jones A. L. Epidermal growth factor: its biologic and metabolic effects with emphasis on the hepatocyte. Hepatology. 1982 Sep-Oct;2(5):601–613. doi: 10.1002/hep.1840020515. [DOI] [PubMed] [Google Scholar]
  37. Tannenbaum G. S., Martin J. B. Evidence for an endogenous ultradian rhythm governing growth hormone secretion in the rat. Endocrinology. 1976 Mar;98(3):562–570. doi: 10.1210/endo-98-3-562. [DOI] [PubMed] [Google Scholar]
  38. Velu T. J., Beguinot L., Vass W. C., Willingham M. C., Merlino G. T., Pastan I., Lowy D. R. Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene. Science. 1987 Dec 4;238(4832):1408–1410. doi: 10.1126/science.3500513. [DOI] [PubMed] [Google Scholar]
  39. Westermark K., Westermark B., Karlsson F. A., Ericson L. E. Location of epidermal growth factor receptors on porcine thyroid follicle cells and receptor regulation by thyrotropin. Endocrinology. 1986 Mar;118(3):1040–1046. doi: 10.1210/endo-118-3-1040. [DOI] [PubMed] [Google Scholar]

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