Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1992 Jan;89(1):184–190. doi: 10.1172/JCI115560

Effects of osmolality on bicarbonate absorption by medullary thick ascending limb of the rat.

D W Good 1
PMCID: PMC442835  PMID: 1729270

Abstract

Previously we demonstrated that arginine vasopressin (AVP) directly inhibits bicarbonate absorption (JHCO3, pmol/min per mm) in the medullary thick ascending limb (MTAL) of the rat. To determine whether changes in osmolality also may affect bicarbonate absorption, MTAL were studied in vitro with 25 mM HCO3- solutions. Control osmolality was 290 mosmol/kg H2O. In the absence of AVP, increasing osmolality to 560 in perfusate and bath by addition of 150 mM NaCl reduced JHCO3 from 13.7 to 4.5. With 2 x 10(-10) M AVP in the bath, adding 150 mM NaCl to perfusate and bath reduced JHCO3 from 6.9 to 0.6, while adding NaCl to the bath alone reduced JHCO3 from 7.1 to 0.5. Adding 150 mM NaCl to perfusate and bath caused a similar inhibition of JHCO3 in MTAL perfused with furosemide to inhibit net NaCl absorption. In the presence of AVP, adding 600 mM urea to perfusate and bath inhibited JHCO3 by 55%; adding 300 or 600 mM mannitol to perfusate and bath inhibited JHCO3 by 75%. The effects on JHCO3 were reversible and dissociable from changes in transepithelial voltage. Conclusions: (1) osmolality is a factor capable of regulating renal tubule bicarbonate absorption; (2) hypertonicity produced with NaCl, urea, or mannitol markedly inhibits bicarbonate absorption in the MTAL; (3) this inhibition occurs independent of, and is additive to, inhibition by vasopressin. Hypertonicity may shift TAL HCO3- absorption from medulla to cortex, thereby limiting delivery of bicarbonate to the medullary interstitium during antidiuresis.

Full text

PDF
184

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bosch J. P., Goldstein M. H., Levitt M. F., Kahn T. Effect of chronic furosemide administration on hydrogen and sodium excretion in the dog. Am J Physiol. 1977 May;232(5):F397–F404. doi: 10.1152/ajprenal.1977.232.5.F397. [DOI] [PubMed] [Google Scholar]
  2. DuBose T. D., Jr, Good D. W. Effects of diuretics on renal acid-base transport. Semin Nephrol. 1988 Sep;8(3):282–294. [PubMed] [Google Scholar]
  3. Good D. W. Adaptation of HCO-3 and NH+4 transport in rat MTAL: effects of chronic metabolic acidosis and Na+ intake. Am J Physiol. 1990 May;258(5 Pt 2):F1345–F1353. doi: 10.1152/ajprenal.1990.258.5.F1345. [DOI] [PubMed] [Google Scholar]
  4. Good D. W. Bicarbonate absorption by the thick ascending limb of Henle's loop. Semin Nephrol. 1990 Mar;10(2):132–138. [PubMed] [Google Scholar]
  5. Good D. W. Effects of potassium on ammonia transport by medullary thick ascending limb of the rat. J Clin Invest. 1987 Nov;80(5):1358–1365. doi: 10.1172/JCI113213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Good D. W. Inhibition of bicarbonate absorption by peptide hormones and cyclic adenosine monophosphate in rat medullary thick ascending limb. J Clin Invest. 1990 Apr;85(4):1006–1013. doi: 10.1172/JCI114530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Good D. W., Knepper M. A., Burg M. B. Ammonia and bicarbonate transport by thick ascending limb of rat kidney. Am J Physiol. 1984 Jul;247(1 Pt 2):F35–F44. doi: 10.1152/ajprenal.1984.247.1.F35. [DOI] [PubMed] [Google Scholar]
  8. Good D. W. Sodium-dependent bicarbonate absorption by cortical thick ascending limb of rat kidney. Am J Physiol. 1985 Jun;248(6 Pt 2):F821–F829. doi: 10.1152/ajprenal.1985.248.6.F821. [DOI] [PubMed] [Google Scholar]
  9. Good D. W., Wright F. S. Luminal influences on potassium secretion: transepithelial voltage. Am J Physiol. 1980 Sep;239(3):F289–F298. doi: 10.1152/ajprenal.1980.239.3.F289. [DOI] [PubMed] [Google Scholar]
  10. Hebert S. C., Culpepper R. M., Andreoli T. E. NaCl transport in mouse medullary thick ascending limbs. III. Modulation of the ADH effect by peritubular osmolality. Am J Physiol. 1981 Oct;241(4):F443–F451. doi: 10.1152/ajprenal.1981.241.4.F443. [DOI] [PubMed] [Google Scholar]
  11. Hebert S. C. Hypertonic cell volume regulation in mouse thick limbs. I. ADH dependency and nephron heterogeneity. Am J Physiol. 1986 Jun;250(6 Pt 1):C907–C919. doi: 10.1152/ajpcell.1986.250.6.C907. [DOI] [PubMed] [Google Scholar]
  12. Hebert S. C. Hypertonic cell volume regulation in mouse thick limbs. II. Na+-H+ and Cl(-)-HCO3- exchange in basolateral membranes. Am J Physiol. 1986 Jun;250(6 Pt 1):C920–C931. doi: 10.1152/ajpcell.1986.250.6.C920. [DOI] [PubMed] [Google Scholar]
  13. Hebert S. C., Reeves W. B., Molony D. A., Andreoli T. E. The medullary thick limb: function and modulation of the single-effect multiplier. Kidney Int. 1987 Feb;31(2):580–589. doi: 10.1038/ki.1987.38. [DOI] [PubMed] [Google Scholar]
  14. Hropot M., Fowler N., Karlmark B., Giebisch G. Tubular action of diuretics: distal effects on electrolyte transport and acidification. Kidney Int. 1985 Sep;28(3):477–489. doi: 10.1038/ki.1985.154. [DOI] [PubMed] [Google Scholar]
  15. Knepper M. A. Urea transport in isolated thick ascending limbs and collecting ducts from rats. Am J Physiol. 1983 Nov;245(5 Pt 1):F634–F639. doi: 10.1152/ajprenal.1983.245.5.F634. [DOI] [PubMed] [Google Scholar]
  16. Mackovic-Basic M., Kurtz I. H+/base transport in the proximal straight tubule and thin descending limb of Henle. Semin Nephrol. 1990 Mar;10(2):122–131. [PubMed] [Google Scholar]
  17. Paillard M., Bichara M. Peptide hormone effects on urinary acidification and acid-base balance: PTH, ADH, and glucagon. Am J Physiol. 1989 Jun;256(6 Pt 2):F973–F985. doi: 10.1152/ajprenal.1989.256.6.F973. [DOI] [PubMed] [Google Scholar]
  18. Rastogi S., Bayliss J. M., Nascimento L., Arruda J. A. Hyperkalemic renal tubular acidosis: effect of furosemide in humans and in rats. Kidney Int. 1985 Nov;28(5):801–807. doi: 10.1038/ki.1985.201. [DOI] [PubMed] [Google Scholar]
  19. Sasaki S., Imai M. Effects of vasopressin on water and NaCl transport across the in vitro perfused medullary thick ascending limb of Henle's loop of mouse, rat, and rabbit kidneys. Pflugers Arch. 1980 Feb;383(3):215–221. doi: 10.1007/BF00587521. [DOI] [PubMed] [Google Scholar]
  20. Sebastian A., Schambelan M., Sutton J. M. Amelioration of hyperchloremic acidosis with furosemide therapy in patients with chronic renal insufficiency and type 4 renal tubular acidosis. Am J Nephrol. 1984;4(5):287–300. doi: 10.1159/000166827. [DOI] [PubMed] [Google Scholar]
  21. Tomita K., Pisano J. J., Burg M. B., Knepper M. A. Effects of vasopressin and bradykinin on anion transport by the rat cortical collecting duct. Evidence for an electroneutral sodium chloride transport pathway. J Clin Invest. 1986 Jan;77(1):136–141. doi: 10.1172/JCI112268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Torikai S., Imai M. Effects of solute concentration on vasopressin stimulated cyclic AMP generation in the rat medullary thick ascending limbs of Henle's loop. Pflugers Arch. 1984 Mar;400(3):306–308. doi: 10.1007/BF00581564. [DOI] [PubMed] [Google Scholar]
  23. Wall S. M., Sands J. M., Flessner M. F., Nonoguchi H., Spring K. R., Knepper M. A. Net acid transport by isolated perfused inner medullary collecting ducts. Am J Physiol. 1990 Jan;258(1 Pt 2):F75–F84. doi: 10.1152/ajprenal.1990.258.1.F75. [DOI] [PubMed] [Google Scholar]
  24. Winters C. J., Reeves W. B., Andreoli T. E. A survey of transport properties of the thick ascending limb. Semin Nephrol. 1991 Mar;11(2):236–247. [PubMed] [Google Scholar]
  25. Work J., Galla J. H., Booker B. B., Schafer J. A., Luke R. G. Effect of ADH on chloride reabsorption in the loop of Henle of the Brattleboro rat. Am J Physiol. 1985 Nov;249(5 Pt 2):F698–F703. doi: 10.1152/ajprenal.1985.249.5.F698. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES