Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1992 Apr;89(4):1282–1287. doi: 10.1172/JCI115713

Ultraviolet-irradiated monocytes efficiently inhibit the intracellular replication of Mycobacterium avium intracellulare.

W S Mirando 1, H Shiratsuchi 1, K Tubesing 1, H Toba 1, J J Ellner 1, C A Elmets 1
PMCID: PMC442989  PMID: 1556188

Abstract

The purpose of this study was to evaluate the effect of ultraviolet (UV) radiation on the antimicrobial activities of monocytes for the intracellular pathogen Mycobacterium avium intracellulare (MAI). UV radiation augmented monocyte antimicrobial activity for MAI in a dose-dependent fashion. UVB doses of greater than or equal to 25 J/m2 resulted in a 50-100-fold reduction in MAI growth 7 d after initiation of culture. The increased monocyte antibacterial effect could be blocked by a plate glass filter, indicating that wavelengths within the UVB were responsible for the effect. UV radiation did not stimulate monocyte phagocytosis, and enhanced inhibition of MAI growth was observed in populations of adherent mononuclear cells that were devoid of T cells. This suggested that UV radiation acted directly to augment intrinsic monocyte antimicrobial activities. The administration of 8-methoxypsoralen plus UVA radiation to monocytes also augmented their antimicrobial activities against MAI. UV radiation thus may serve as a unique agent by which to evaluate the mechanisms by which mononuclear phagocytes control the growth of MAI.

Full text

PDF
1282

Images in this article

1285Image
on p.1285
1285Image
on p.1285

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahn C. H., Ahn S. S., Anderson R. A., Murphy D. T., Mammo A. A four-drug regimen for initial treatment of cavitary disease caused by Mycobacterium avium complex. Am Rev Respir Dis. 1986 Sep;134(3):438–441. doi: 10.1164/arrd.1986.134.3.438. [DOI] [PubMed] [Google Scholar]
  2. Bermudez L. E., Stevens P., Kolonoski P., Wu M., Young L. S. Treatment of experimental disseminated Mycobacterium avium complex infection in mice with recombinant IL-2 and tumor necrosis factor. J Immunol. 1989 Nov 1;143(9):2996–3000. [PubMed] [Google Scholar]
  3. Bhardwaj N., Nash T. W., Horwitz M. A. Interferon-gamma-activated human monocytes inhibit the intracellular multiplication of Legionella pneumophila. J Immunol. 1986 Oct 15;137(8):2662–2669. [PubMed] [Google Scholar]
  4. Crowle A. J., Ross E. J., May M. H. Inhibition by 1,25(OH)2-vitamin D3 of the multiplication of virulent tubercle bacilli in cultured human macrophages. Infect Immun. 1987 Dec;55(12):2945–2950. doi: 10.1128/iai.55.12.2945-2950.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crowle A. J., Sbarbaro J. A., Judson F. N., Douvas G. S., May M. H. Inhibition by streptomycin of tubercle bacilli within cultured human macrophages. Am Rev Respir Dis. 1984 Nov;130(5):839–844. doi: 10.1164/arrd.1984.130.5.839. [DOI] [PubMed] [Google Scholar]
  6. Davidson P. T., Khanijo V., Goble M., Moulding T. S. Treatment of disease due to Mycobacterium intracellulare. Rev Infect Dis. 1981 Sep-Oct;3(5):1052–1059. doi: 10.1093/clinids/3.5.1052. [DOI] [PubMed] [Google Scholar]
  7. Denkins Y., Fidler I. J., Kripke M. L. Exposure of mice to UV-B radiation suppresses delayed hypersensitivity to Candida albicans. Photochem Photobiol. 1989 May;49(5):615–619. doi: 10.1111/j.1751-1097.1989.tb08432.x. [DOI] [PubMed] [Google Scholar]
  8. Dutt A. K., Stead W. W. Long-term results of medical treatment in Mycobacterium intracellulare infection. Am J Med. 1979 Sep;67(3):449–453. doi: 10.1016/0002-9343(79)90792-7. [DOI] [PubMed] [Google Scholar]
  9. Ellner J. J., Wallis R. S. Immunologic aspects of mycobacterial infections. Rev Infect Dis. 1989 Mar-Apr;11 (Suppl 2):S455–S459. doi: 10.1093/clinids/11.supplement_2.s455. [DOI] [PubMed] [Google Scholar]
  10. Etzkorn E. T., Aldarondo S., McAllister C. K., Matthews J., Ognibene A. J. Medical therapy of Mycobacterium avium-intracellulare pulmonary disease. Am Rev Respir Dis. 1986 Sep;134(3):442–445. doi: 10.1164/arrd.1986.134.3.442. [DOI] [PubMed] [Google Scholar]
  11. Gallo J. H., Young G. A., Forrest P. R., Vincent P. C., Jennis F. Disseminated atypical mycobacterial infection in hairy cell leukemia. Pathology. 1983 Jul;15(3):241–245. doi: 10.3109/00313028309083500. [DOI] [PubMed] [Google Scholar]
  12. Giannini M. S. Suppression of pathogenesis in cutaneous leishmaniasis by UV irradiation. Infect Immun. 1986 Mar;51(3):838–843. doi: 10.1128/iai.51.3.838-843.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hawkins C. C., Gold J. W., Whimbey E., Kiehn T. E., Brannon P., Cammarata R., Brown A. E., Armstrong D. Mycobacterium avium complex infections in patients with the acquired immunodeficiency syndrome. Ann Intern Med. 1986 Aug;105(2):184–188. doi: 10.7326/0003-4819-105-2-184. [DOI] [PubMed] [Google Scholar]
  14. Hertl M., Kaplan D. R., Fayen J. D., Panuska J. R., Ellner J. J., Elmets C. A. The accessory function of B lymphocytes is resistant to the adverse effects of UV radiation. Eur J Immunol. 1991 Feb;21(2):291–297. doi: 10.1002/eji.1830210208. [DOI] [PubMed] [Google Scholar]
  15. Jeevan A., Kripke M. L. Effect of a single exposure to ultraviolet radiation on Mycobacterium bovis bacillus Calmette-Guerin infection in mice. J Immunol. 1989 Nov 1;143(9):2837–2843. [PubMed] [Google Scholar]
  16. Johnson J. L., Shiratsuchi H., Toba H., Ellner J. J. Preservation of monocyte effector functions against Mycobacterium avium-M. intracellulare in patients with AIDS. Infect Immun. 1991 Oct;59(10):3639–3645. doi: 10.1128/iai.59.10.3639-3645.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Krutmann J., Elmets C. A. Recent studies on mechanisms in photoimmunology. Photochem Photobiol. 1988 Dec;48(6):787–798. doi: 10.1111/j.1751-1097.1988.tb02894.x. [DOI] [PubMed] [Google Scholar]
  18. Krutmann J., Khan I. U., Wallis R. S., Zhang F., Rich E. A., Ellner J. J., Elmets C. A. Cell membrane is a major locus for ultraviolet B-induced alterations in accessory cells. J Clin Invest. 1990 May;85(5):1529–1536. doi: 10.1172/JCI114600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Murray H. W., Gellene R. A., Libby D. M., Rothermel C. D., Rubin B. Y. Activation of tissue macrophages from AIDS patients: in vitro response of AIDS alveolar macrophages to lymphokines and interferon-gamma. J Immunol. 1985 Oct;135(4):2374–2377. [PubMed] [Google Scholar]
  20. Murray H. W. Interferon-gamma, the activated macrophage, and host defense against microbial challenge. Ann Intern Med. 1988 Apr;108(4):595–608. doi: 10.7326/0003-4819-108-4-595. [DOI] [PubMed] [Google Scholar]
  21. Murray H. W., Rubin B. Y., Masur H., Roberts R. B. Impaired production of lymphokines and immune (gamma) interferon in the acquired immunodeficiency syndrome. N Engl J Med. 1984 Apr 5;310(14):883–889. doi: 10.1056/NEJM198404053101404. [DOI] [PubMed] [Google Scholar]
  22. Murray H. W., Rubin B. Y., Rothermel C. D. Killing of intracellular Leishmania donovani by lymphokine-stimulated human mononuclear phagocytes. Evidence that interferon-gamma is the activating lymphokine. J Clin Invest. 1983 Oct;72(4):1506–1510. doi: 10.1172/JCI111107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Müller I., Pedrazzini T., Farrell J. P., Louis J. T-cell responses and immunity to experimental infection with leishmania major. Annu Rev Immunol. 1989;7:561–578. doi: 10.1146/annurev.iy.07.040189.003021. [DOI] [PubMed] [Google Scholar]
  24. Nathan C. F., Murray H. W., Wiebe M. E., Rubin B. Y. Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity. J Exp Med. 1983 Sep 1;158(3):670–689. doi: 10.1084/jem.158.3.670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Passwell J. H., Shor R., Shoham J. The enhancing effect of interferon-beta and -gamma on the killing of Leishmania tropica major in human mononuclear phagocytes in vitro. J Immunol. 1986 Apr 15;136(8):3062–3066. [PubMed] [Google Scholar]
  26. Rich E. A., Elmets C. A., Fujiwara H., Wallis R. S., Ellner J. J. Deleterious effect of ultraviolet-B radiation on accessory function of human blood adherent mononuclear cells. Clin Exp Immunol. 1987 Oct;70(1):116–126. [PMC free article] [PubMed] [Google Scholar]
  27. Rook G. A., Steele J., Fraher L., Barker S., Karmali R., O'Riordan J., Stanford J. Vitamin D3, gamma interferon, and control of proliferation of Mycobacterium tuberculosis by human monocytes. Immunology. 1986 Jan;57(1):159–163. [PMC free article] [PubMed] [Google Scholar]
  28. Rosenzweig D. Y. Pulmonary mycobacterial infections due to Mycobacterium intracellulare-avium complex. Clinical features and course in 100 consecutive cases. Chest. 1979 Feb;75(2):115–119. doi: 10.1378/chest.75.2.115. [DOI] [PubMed] [Google Scholar]
  29. Rothermel C. D., Rubin B. Y., Murray H. W. Gamma-interferon is the factor in lymphokine that activates human macrophages to inhibit intracellular Chlamydia psittaci replication. J Immunol. 1983 Nov;131(5):2542–2544. [PubMed] [Google Scholar]
  30. Shiratsuchi H., Johnson J. L., Ellner J. J. Bidirectional effects of cytokines on the growth of Mycobacterium avium within human monocytes. J Immunol. 1991 May 1;146(9):3165–3170. [PubMed] [Google Scholar]
  31. Stein B., Rahmsdorf H. J., Steffen A., Litfin M., Herrlich P. UV-induced DNA damage is an intermediate step in UV-induced expression of human immunodeficiency virus type 1, collagenase, c-fos, and metallothionein. Mol Cell Biol. 1989 Nov;9(11):5169–5181. doi: 10.1128/mcb.9.11.5169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Toba H., Crawford J. T., Ellner J. J. Pathogenicity of Mycobacterium avium for human monocytes: absence of macrophage-activating factor activity of gamma interferon. Infect Immun. 1989 Jan;57(1):239–244. doi: 10.1128/iai.57.1.239-244.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Webb A. R., Holick M. F. The role of sunlight in the cutaneous production of vitamin D3. Annu Rev Nutr. 1988;8:375–399. doi: 10.1146/annurev.nu.08.070188.002111. [DOI] [PubMed] [Google Scholar]
  34. Weinstein R. A., Golomb H. M., Grumet G., Gelmann E., Schechter G. P. Hairy cell leukemia: association with disseminated atypical mycobacterial infection. Cancer. 1981 Jul 15;48(2):380–383. doi: 10.1002/1097-0142(19810715)48:2<380::aid-cncr2820480226>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
  35. Wolinsky E. Nontuberculous mycobacteria and associated diseases. Am Rev Respir Dis. 1979 Jan;119(1):107–159. doi: 10.1164/arrd.1979.119.1.107. [DOI] [PubMed] [Google Scholar]
  36. Zakowski P., Fligiel S., Berlin G. W., Johnson L., Jr Disseminated Mycobacterium avium-intracellulare infection in homosexual men dying of acquired immunodeficiency. JAMA. 1982 Dec 10;248(22):2980–2982. doi: 10.1001/jama.1982.03330220024029. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES