There are errors in Table 1. The trans-heterozygous genotypes under ‘etl-1 genotype’ are not displayed in the standard way; ku491+ should be ku491/+, ok1002+ should be ok1002/+ and ku491 ok1002 should be ku491/ok1002. The correct table is displayed underneath.
Table 1. elt-1 mutants have an L4-stage bursting vulva and defective alae formation and elt-1(ku491) is a partial loss-of- function allele.
| Strain | RNAi | L4 Bursting Vulva | L4 Molt Alae | Young Adult Seam Cells | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| % | n | Absent (%) | Gapped (%) | Present (%) | n | SCM | Std Dev | n | ||
| wild-type | Empty vector a | 0.0 | 245 | 0.0 | 0.0 | 100.0 | 27 | 16.0 | 0.4 | 27 |
| daf-12(rh61rh411) | Empty vector a | 0.0 | 183 | 0.0 | 0.0 | 100.0 | 24 | 18.1 | 1.6 | 24 |
| elt-1(ku491) | Empty vector a | 3.6 | 169 | 88.4 | 3.8 | 7.6 | 26 | 12.9 | 2.2 | 15 |
| elt-1(ku491);daf-12(rh61rh411) | Empty vector a | 55.1 | 198 | 88.8 | 0.0 | 11.1 | 18 | 39.7 | 9.8 | 29 |
| wild-type | elt-1 | 48.7 | 117 | 100.0 | 0.0 | 0.0 | 21 | 0.5 | 0.8 | 21 |
| daf-12(rh61rh411) | elt-1 | 56.7 | 90 | 90.0 | 5.0 | 5.0 | 20 | 0.2 | 0.4 | 20 |
| elt-1(ku491) | elt-1 | 76.3 | 114 | 100.0 | 0.0 | 0.0 | 27 | 1.3 | 1.2 | 27 |
| elt-1(ku491);daf-12(rh61rh411) | elt-1 | 94.8 | 231 | 96.4 | 3.6 | 0.0 | 28 | 2.0 | 2.2 | 28 |
| elt-1 genotype b | daf-12 genotype | |||||||||
| ku491/+ | Wild-type | 0.0 | 60 | 0.0 | 0.0 | 100.0 | 19 | 15.9 | 0.3 | 18 |
| ku491/+ | rh61rh411 | 0.0 | 57 | 0.0 | 0.0 | 100.0 | 18 | 18.3 | 1.8 | 18 |
| ok1002/+ | Wild-type | 0.0 | 92 | 0.0 | 0.0 | 100.0 | 18 | 16.0 | 0.3 | 18 |
| ok1002/+ | rh61rh411 | 0.0 | 78 | 0.0 | 0.0 | 100.0 | 17 | 18.2 | 1.9 | 17 |
| ku491/ok1002 | Wild-type | 1.9 | 52 | 84.0 | 16.0 | 0.0 | 25 | 9.4 c | 2.0 | 25 |
| ku491/ok1002 | rh61rh411 | 65.9 | 41 | 100.0 | 0.0 | 0.0 | 20 | 18.2 d | 6.6 | 20 |
aPhenotypes on empty-vector control RNAi were similar to the standard E. coli strain OP50.
bAn allele of elt-1(ku491) linked to mutations in unc-24 and dpy-20 was used for these strains.
cFor elt-1(ku491) animals vs elt-1(ku491)-over-elt-1(null) animals at the young adult stage, the p-value for the comparison of seam-cell numbers is 0.0340
dFor elt-1(ku491);daf-12(rh61rh411) animals vs elt-1(ku491)-over-elt-1(null);daf-12(rh61rh411) animals at the young adult stage, the p-value for the comparison of seam-cell numbers is < 0.0001.
Reference
- 1. Cohen ML, Kim S, Morita K, Kim SH, Han M (2015) The GATA Factor elt-1 Regulates C. elegans Developmental Timing by Promoting Expression of the let-7 Family MicroRNAs. PLoS Genet 11(3): e1005099 doi: 10.1371/journal.pgen.1005099 [DOI] [PMC free article] [PubMed] [Google Scholar]
