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. 2014 Dec 23;29(5):1092–1103. doi: 10.1038/leu.2014.331

Figure 2.

Figure 2

Effects of SF3B1 knockdown on gene expression and splicing. (a) ABCB7 and FTMT expression levels in TF1, K562, HEL and SKM1 cells with SF3B1 knockdown, as measured by qRT-PCR 48 h post transfection. Each cell line transfected with siRNA targeting SF3B1 was compared with the corresponding cell line transfected with the scramble control. (b) Reverse transcription-PCR of TP53 exons 5–7 showing aberrant splicing in K562 cells with SF3B1 knockdown (siRNA) compared with scramble (SCR). (c, d). qRT-PCR analysis using primers that monitor general gene expression (GE) in a constitutive exon (Ex4 of CCNA2 and Ex3 of STK6) or primers specific for splice junctions corresponding to exon inclusion or skipping in the cyclin A2 (CCNA2) and Aurora Kinase A (STK6) genes in K562 cells. Cells with SF3B1 knockdown (siRNA) show alternative splicing events. Results in subpanel a were obtained from scramble n=3 and SF3B1 siRNA n=4. Results in subpanel c–d were obtained from scramble n=2 and SF3B1 siRNA n=3. *P<0.05.