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. Author manuscript; available in PMC: 2016 May 13.
Published in final edited form as: Metallomics. 2015 Mar 13;7(5):847–856. doi: 10.1039/c5mt00052a

Fig. 3. Overexpression of fpn-1.1 in pdr-1 mutants decreases levels of highly pro-oxidant metals.

Fig. 3

(A-D) Intraworm metal concentrations following acute, 30 min MnCl2 treatment (0, 2.5 and 5 mM) at the L1 stage, as quantified by ICP-MS/MS. (A) Mn content (μg Mn/mg protein) in pdr-1 KO and pdr-1 KO; fpn-1.1 OVR animals. (B) Iron (Fe) content (μg Fe/mg protein) in pdr-1 KO and pdr-1 KO; fpn-1.1 OVR animals. (C) Zinc (Zn) content (μg Zn/mg protein) in pdr-1 KO and pdr-1 KO; fpn-1.1 OVR animals. (D) Copper (Cu) content (μg Cu/mg protein) in pdr-1 KO and pdr-1 KO; fpn-1.1 OVR animals. (A-D) Data are expressed as mean values + SEM from at least six independent experiments and normalized to total protein content. Statistical analysis by two-way ANOVA: (A) interaction, ns; genotype, ns; concentration, p=0.0165; (B) interaction, ns; genotype, p=0.0092; concentration, ns; (C) interaction, ns; genotype, ns; concentration, ns; (D) interaction, ns; genotype, p=0.0256; concentration, ns.