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. 1994 Jul 19;91(15):7012–7016. doi: 10.1073/pnas.91.15.7012

JIP60, a methyl jasmonate-induced ribosome-inactivating protein involved in plant stress reactions.

S Reinbothe 1, C Reinbothe 1, J Lehmann 1, W Becker 1, K Apel 1, B Parthier 1
PMCID: PMC44328  PMID: 8041737

Abstract

Plant tissues treated with the naturally occurring cyclopentanone compound methyl jasmonate or exposed to stress causing in planta jasmonate accumulation express distinctive proteins and, concomitantly, reduce the synthesis of most preexisting proteins. One of the recently identified jasmonate-induced proteins, designated JIP60, in barley is a ribosome-inactivating protein that cleaves polysomes of both animal and plant origin into their ribosomal subunits. By attacking foreign and self ribosomes, respectively, JIP60 appears to be both a defense protein and a potent regulator of protein synthesis in stressed plant tissues.

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Selected References

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  1. Andresen I., Becker W., Schlüter K., Burges J., Parthier B., Apel K. The identification of leaf thionin as one of the main jasmonate-induced proteins of barley (Hordeum vulgare). Plant Mol Biol. 1992 May;19(2):193–204. doi: 10.1007/BF00027341. [DOI] [PubMed] [Google Scholar]
  2. Becker W., Apel K. Isolation and characterization of a cDNA clone encoding a novel jasmonate-induced protein of barley (Hordeum vulgare L.). Plant Mol Biol. 1992 Sep;19(6):1065–1067. doi: 10.1007/BF00040538. [DOI] [PubMed] [Google Scholar]
  3. Benatti L., Saccardo M. B., Dani M., Nitti G., Sassano M., Lorenzetti R., Lappi D. A., Soria M. Nucleotide sequence of cDNA coding for saporin-6, a type-1 ribosome-inactivating protein from Saponaria officinalis. Eur J Biochem. 1989 Aug 1;183(2):465–470. doi: 10.1111/j.1432-1033.1989.tb14951.x. [DOI] [PubMed] [Google Scholar]
  4. Bohlmann H., Clausen S., Behnke S., Giese H., Hiller C., Reimann-Philipp U., Schrader G., Barkholt V., Apel K. Leaf-specific thionins of barley-a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defence mechanism of plants. EMBO J. 1988 Jun;7(6):1559–1565. doi: 10.1002/j.1460-2075.1988.tb02980.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brigotti M., Rambelli F., Zamboni M., Montanaro L., Sperti S. Effect of alpha-sarcin and ribosome-inactivating proteins on the interaction of elongation factors with ribosomes. Biochem J. 1989 Feb 1;257(3):723–727. doi: 10.1042/bj2570723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Coleman W. H., Roberts W. K. Inhibitors of animal cell-free protein synthesis from grains. Biochim Biophys Acta. 1982 Mar 29;696(3):239–244. doi: 10.1016/0167-4781(82)90053-7. [DOI] [PubMed] [Google Scholar]
  7. Creelman R. A., Tierney M. L., Mullet J. E. Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):4938–4941. doi: 10.1073/pnas.89.11.4938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Endo Y., Mitsui K., Motizuki M., Tsurugi K. The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes. The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins. J Biol Chem. 1987 Apr 25;262(12):5908–5912. [PubMed] [Google Scholar]
  9. Endo Y., Tsurugi K. RNA N-glycosidase activity of ricin A-chain. Mechanism of action of the toxic lectin ricin on eukaryotic ribosomes. J Biol Chem. 1987 Jun 15;262(17):8128–8130. [PubMed] [Google Scholar]
  10. Farmer E. E., Johnson R. R., Ryan C. A. Regulation of expression of proteinase inhibitor genes by methyl jasmonate and jasmonic Acid. Plant Physiol. 1992 Mar;98(3):995–1002. doi: 10.1104/pp.98.3.995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Farmer E. E., Ryan C. A. Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proc Natl Acad Sci U S A. 1990 Oct;87(19):7713–7716. doi: 10.1073/pnas.87.19.7713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gollmer I., Apel K. The phytochrome-controlled accumulation of mRNA sequences encoding the light-harvesting chlorophyll a/b protein of barley (Hordeum vulgare L.). Eur J Biochem. 1983 Jun 15;133(2):309–313. doi: 10.1111/j.1432-1033.1983.tb07463.x. [DOI] [PubMed] [Google Scholar]
  13. Gundlach H., Müller M. J., Kutchan T. M., Zenk M. H. Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2389–2393. doi: 10.1073/pnas.89.6.2389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Habuka N., Murakami Y., Noma M., Kudo T., Horikoshi K. Amino acid sequence of Mirabilis antiviral protein, total synthesis of its gene and expression in Escherichia coli. J Biol Chem. 1989 Apr 25;264(12):6629–6637. [PubMed] [Google Scholar]
  15. Katzin B. J., Collins E. J., Robertus J. D. Structure of ricin A-chain at 2.5 A. Proteins. 1991;10(3):251–259. doi: 10.1002/prot.340100309. [DOI] [PubMed] [Google Scholar]
  16. Klein R. R., Mason H. S., Mullet J. E. Light-regulated translation of chloroplast proteins. I. Transcripts of psaA-psaB, psbA, and rbcL are associated with polysomes in dark-grown and illuminated barley seedlings. J Cell Biol. 1988 Feb;106(2):289–301. doi: 10.1083/jcb.106.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Krieg P. A., Melton D. A. Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acids Res. 1984 Sep 25;12(18):7057–7070. doi: 10.1093/nar/12.18.7057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  19. Lamb F. I., Roberts L. M., Lord J. M. Nucleotide sequence of cloned cDNA coding for preproricin. Eur J Biochem. 1985 Apr 15;148(2):265–270. doi: 10.1111/j.1432-1033.1985.tb08834.x. [DOI] [PubMed] [Google Scholar]
  20. Mueller M. J., Brodschelm W., Spannagl E., Zenk M. H. Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid. Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7490–7494. doi: 10.1073/pnas.90.16.7490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Palmiter R. D. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid. Biochemistry. 1974 Aug 13;13(17):3606–3615. doi: 10.1021/bi00714a032. [DOI] [PubMed] [Google Scholar]
  22. Pelham H. R., Jackson R. J. An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem. 1976 Aug 1;67(1):247–256. doi: 10.1111/j.1432-1033.1976.tb10656.x. [DOI] [PubMed] [Google Scholar]
  23. Reinbothe S., Reinbothe C., Heintzen C., Seidenbecher C., Parthier B. A methyl jasmonate-induced shift in the length of the 5' untranslated region impairs translation of the plastid rbcL transcript in barley. EMBO J. 1993 Apr;12(4):1505–1512. doi: 10.1002/j.1460-2075.1993.tb05794.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Reinbothe S., Reinbothe C., Parthier B. Methyl jasmonate-regulated translation of nuclear-encoded chloroplast proteins in barley (Hordeum vulgare L. cv. salome). J Biol Chem. 1993 May 15;268(14):10606–10611. [PubMed] [Google Scholar]
  25. Roberts W. K., Stewart T. S. Purification and properties of a translation inhibitor from wheat germ. Biochemistry. 1979 Jun 12;18(12):2615–2621. doi: 10.1021/bi00579a028. [DOI] [PubMed] [Google Scholar]
  26. Stirpe F., Barbieri L. Ribosome-inactivating proteins up to date. FEBS Lett. 1986 Jan 20;195(1-2):1–8. doi: 10.1016/0014-5793(86)80118-1. [DOI] [PubMed] [Google Scholar]
  27. Vassart G., Dumont J. E., Cantraine F. R. Translational control of protein synthesis: a simulation study. Biochim Biophys Acta. 1971 Oct;247(3):471–485. doi: 10.1016/0005-2787(71)90034-7. [DOI] [PubMed] [Google Scholar]

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