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. 1993 Jun;91(6):2532–2538. doi: 10.1172/JCI116489

Plasma lipoproteins after triglyceride clearance in cholesterol-fed rats.

S H Quarfordt 1, B S Oswald 1, M O Farouk 1, D C Wehrenberg 1, E B Morton 1, B A Landis 1
PMCID: PMC443314  PMID: 8514865

Abstract

The clearance of particulate triglyceride from the plasma of cholesterol-fed rats with appreciable stores of hepatic cholesterol ester produces a substantial increment in plasma cholesterol. Most of this plasma cholesterol increment arises from existing tissue sources. The increment begins from 4 to 6 h after clearance and is due to the appearance of larger cholesterol-rich, triglyceride-poor, beta migrating lipoproteins, which are isolated in the d < 1.063 fraction with an apoprotein (Apo) content consisting primarily of Apo E and smaller amounts of Apo B. A concurrent decrease in alpha lipoproteins occurs with the beta lipoprotein increment. Within 1 d of clearance the beta lipoproteins fall and alpha lipoproteins increase. The increase in total plasma Apo E and Apo B initially parallels that of the cholesterol, but it persists even when cholesterol falls. A modest decrease in plasma Apo A1 was observed during the time alpha lipoproteins declined. A significant increase in plasma lecithin cholesterol acyl transferase preceded the increase in beta lipoprotein cholesterol. This enzyme increment was absent in rats with little lipoprotein response despite increased hepatic cholesterol. In vivo inhibition of this enzyme with dithionitrobenzoic acid virtually eliminated the postclearance hypercholesterolemia. Plasma particulate triglyceride clearance induces an increase in beta lipoproteins. Coupling of this clearance and hepatic lipoprotein secretion occurs by an unknown mechanism modulated by lecithin cholesterol acyl transferase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albers J. J., Chen C. H., Lacko A. G. Isolation, characterization, and assay of lecithin-cholesterol acyltransferase. Methods Enzymol. 1986;129:763–783. doi: 10.1016/0076-6879(86)29103-x. [DOI] [PubMed] [Google Scholar]
  2. Brown M. S., Goldstein J. L. Lipoprotein receptors in the liver. Control signals for plasma cholesterol traffic. J Clin Invest. 1983 Sep;72(3):743–747. doi: 10.1172/JCI111044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DOLE V. P. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J Clin Invest. 1956 Feb;35(2):150–154. doi: 10.1172/JCI103259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dashti N. The effect of low density lipoproteins, cholesterol, and 25-hydroxycholesterol on apolipoprotein B gene expression in HepG2 cells. J Biol Chem. 1992 Apr 5;267(10):7160–7169. [PubMed] [Google Scholar]
  5. Faergeman O., Sata T., Kane J. P., Havel R. J. Metabolism of apoprotein B of plasma very low density lipoproteins in the rat. J Clin Invest. 1975 Dec;56(6):1396–1403. doi: 10.1172/JCI108220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fox J. C., McGill H. C., Jr, Carey K. D., Getz G. S. In vivo regulation of hepatic LDL receptor mRNA in the baboon. Differential effects of saturated and unsaturated fat. J Biol Chem. 1987 May 25;262(15):7014–7020. [PubMed] [Google Scholar]
  7. Glomset J. A. The plasma lecithins:cholesterol acyltransferase reaction. J Lipid Res. 1968 Mar;9(2):155–167. [PubMed] [Google Scholar]
  8. Goodman D. S. Cholesterol ester metabolism. Physiol Rev. 1965 Oct;45(4):747–839. doi: 10.1152/physrev.1965.45.4.747. [DOI] [PubMed] [Google Scholar]
  9. HAVEL R. J., EDER H. A., BRAGDON J. H. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest. 1955 Sep;34(9):1345–1353. doi: 10.1172/JCI103182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones D. Y., Judd J. T., Taylor P. R., Campbell W. S., Nair P. P. Influence of caloric contribution and saturation of dietary fat on plasma lipids in premenopausal women. Am J Clin Nutr. 1987 Jun;45(6):1451–1456. doi: 10.1093/ajcn/45.6.1451. [DOI] [PubMed] [Google Scholar]
  11. KEYS A. Human atherosclerosis and the diet. Circulation. 1952 Jan;5(1):115–118. doi: 10.1161/01.cir.5.1.115. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
  14. Marcel Y. L. Lecithin: cholesterol acyltransferase and intravascular cholesterol transport. Adv Lipid Res. 1982;19:85–136. doi: 10.1016/b978-0-12-024919-0.50009-6. [DOI] [PubMed] [Google Scholar]
  15. Marcel Y. L., Vezina C. Lecithin: cholesterol acyltransferase of human plasma. Role of chylomicrons, very low, and high density lipoproteins in the reaction. J Biol Chem. 1973 Dec 10;248(23):8254–8259. [PubMed] [Google Scholar]
  16. Quarfordt S. H., Oswald B. S., Landis B. A., DeFaria E., Yamaguchi Y. Rat plasma cholesterol after particulate triacylglycerol clearance. Biochim Biophys Acta. 1991 Apr 3;1082(3):247–250. doi: 10.1016/0005-2760(91)90199-r. [DOI] [PubMed] [Google Scholar]
  17. Scanu A., Toth J., Edelstein C., Koga S., Stiller E. Fractionation of human serum high density lipoprotein in urea solutions. Evidence for polypeptide heterogeneity. Biochemistry. 1969 Aug;8(8):3309–3316. doi: 10.1021/bi00836a027. [DOI] [PubMed] [Google Scholar]
  18. Shelburne F. A., Quarfordt S. H. The interaction of heparin with an apoprotein of human very low density lipoprotein. J Clin Invest. 1977 Oct;60(4):944–950. doi: 10.1172/JCI108849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sniderman A., Shapiro S., Marpole D., Skinner B., Teng B., Kwiterovich P. O., Jr Association of coronary atherosclerosis with hyperapobetalipoproteinemia [increased protein but normal cholesterol levels in human plasma low density (beta) lipoproteins]. Proc Natl Acad Sci U S A. 1980 Jan;77(1):604–608. doi: 10.1073/pnas.77.1.604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Spady D. K., Dietschy J. M. Dietary saturated triacylglycerols suppress hepatic low density lipoprotein receptor activity in the hamster. Proc Natl Acad Sci U S A. 1985 Jul;82(13):4526–4530. doi: 10.1073/pnas.82.13.4526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tall A. R., Green P. H., Glickman R. M., Riley J. W. Metabolic fate of chylomicron phospholipids and apoproteins in the rat. J Clin Invest. 1979 Oct;64(4):977–989. doi: 10.1172/JCI109564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

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