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. 1989 Feb 25;17(4):1649–1663. doi: 10.1093/nar/17.4.1649

Processing pathway of Escherichia coli 16S precursor rRNA.

A K Srivastava 1, D Schlessinger 1
PMCID: PMC443564  PMID: 2646597

Abstract

Immediate precursors of 16S rRNA are processed by endonucleolytic cleavage at both 5' and 3' mature termini, with the concomitant release of precursor fragments which are further metabolized by both exo- and endonucleases. In wild-type cells rapid cleavages by RNase III in precursor-specific sequences precede the subsequent formation of the mature ends; mature termini can, however, be formed directly from pre-16S rRNA with no intermediate species. The direct maturation is most evident in a strain deficient in RNase III, and the results in whole cells are consistent with results from maturation reactions in vitro. Thus, maturation does not require cleavages within the double-stranded stems that enclose mature rRNA sequences in the pre-16S rRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol. 1981 May 15;148(2):107–127. doi: 10.1016/0022-2836(81)90508-8. [DOI] [PubMed] [Google Scholar]
  2. Ceccarelli A., Dotto G. P., Altruda F., Perlo C., Silengo L., Turco E., Mangiarotti G. Immature 50 S subunits in Escherichia coli polyribosomes. FEBS Lett. 1978 Sep 15;93(2):348–350. doi: 10.1016/0014-5793(78)81137-5. [DOI] [PubMed] [Google Scholar]
  3. Chase J. W., Richardson C. C. Exonuclease VII of Escherichia coli. Mechanism of action. J Biol Chem. 1974 Jul 25;249(14):4553–4561. [PubMed] [Google Scholar]
  4. Chase J. W., Richardson C. C. Exonuclease VII of Escherichia coli. Purification and properties. J Biol Chem. 1974 Jul 25;249(14):4545–4552. [PubMed] [Google Scholar]
  5. Corte G., Schlessinger D., Longo D., Venkov P. Transformation of 17 s to 16 s ribosomal RNA using ribonuclease II of Escherichia coli. J Mol Biol. 1971 Sep 14;60(2):325–338. doi: 10.1016/0022-2836(71)90297-x. [DOI] [PubMed] [Google Scholar]
  6. Dahlberg A. E., Dahlberg J. E., Lund E., Tokimatsu H., Rabson A. B., Calvert P. C., Reynolds F., Zahalak M. Processing of the 5' end of Escherichia coli 16S ribosomal RNA. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3598–3602. doi: 10.1073/pnas.75.8.3598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Feunteun J., Jordan B. R., Monier R. Study of the maturation of 5 s RNA precursors in Escherichia coli. J Mol Biol. 1972 Oct 14;70(3):465–474. doi: 10.1016/0022-2836(72)90553-0. [DOI] [PubMed] [Google Scholar]
  8. Gesteland R. F. Isolation and characterization of ribonuclease I mutants of Escherichia coli. J Mol Biol. 1966 Mar;16(1):67–84. doi: 10.1016/s0022-2836(66)80263-2. [DOI] [PubMed] [Google Scholar]
  9. Hayes F., Vasseur M. Processing of the 17-S Escherichia coli precursor RNA in the 27-S pre-ribosomal particle. Eur J Biochem. 1976 Jan 15;61(2):433–442. doi: 10.1111/j.1432-1033.1976.tb10037.x. [DOI] [PubMed] [Google Scholar]
  10. King T. C., Schlessinger D. S1 nuclease mapping analysis of ribosomal RNA processing in wild type and processing deficient Escherichia coli. J Biol Chem. 1983 Oct 10;258(19):12034–12042. [PubMed] [Google Scholar]
  11. King T. C., Sirdeshmukh R., Schlessinger D. RNase III cleavage is obligate for maturation but not for function of Escherichia coli pre-23S rRNA. Proc Natl Acad Sci U S A. 1984 Jan;81(1):185–188. doi: 10.1073/pnas.81.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. King T. C., Sirdeskmukh R., Schlessinger D. Nucleolytic processing of ribonucleic acid transcripts in procaryotes. Microbiol Rev. 1986 Dec;50(4):428–451. doi: 10.1128/mr.50.4.428-451.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Klein B. K., Staden A., Schlessinger D. Electron microscopy of secondary structure in partially denatured precursor and mature Escherichia coli 16 S and 23 S rRNA. J Biol Chem. 1985 Jul 5;260(13):8114–8120. [PubMed] [Google Scholar]
  14. Krych M., Sirdeshmukh R., Gourse R., Schlessinger D. Processing of Escherichia coli 16S rRNA with bacteriophage lambda leader sequences. J Bacteriol. 1987 Dec;169(12):5523–5529. doi: 10.1128/jb.169.12.5523-5529.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mangiarotti G., Turco E., Ponzetto A., Altruda F. Precursor 16S RNA in active 30S ribosomes. Nature. 1974 Jan 18;247(5437):147–148. doi: 10.1038/247147a0. [DOI] [PubMed] [Google Scholar]
  16. Sirdeshmukh R., Schlessinger D. Ordered processing of Escherichia coli 23S rRNA in vitro. Nucleic Acids Res. 1985 Jul 25;13(14):5041–5054. doi: 10.1093/nar/13.14.5041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Srivastava A. K., Schlessinger D. Coregulation of processing and translation: mature 5' termini of Escherichia coli 23S ribosomal RNA form in polysomes. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7144–7148. doi: 10.1073/pnas.85.19.7144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wireman J. W., Sypherd P. S. In vitro assembly of 30S ribosomal particles from precursor 16S RNA of Escherichia coli. Nature. 1974 Feb 22;247(5442):552–554. doi: 10.1038/247552a0. [DOI] [PubMed] [Google Scholar]
  19. Young R. A., Steitz J. A. Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3593–3597. doi: 10.1073/pnas.75.8.3593. [DOI] [PMC free article] [PubMed] [Google Scholar]

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