Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun;21(6):1159–1172. doi: 10.1261/rna.045559.114

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Liu et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

FIGURE 3. — Shad1 modification and correlation of expression with YB-1. (A) The Shad1 RNAs possess a 5′-phosphate as indicated by a 5′-phosphate-dependent exonuclease reaction. (B) Periodate oxidation followed by polyadenylation and qPCR reveals 3′ modifications in YB-1–associated RNAs and the positive control, miR-135m, a synthetic oligo with a 3′ end 2′-O-methyl modification. (C) Genomic location of Shad1 shyRNA and smyRNA as determined by sequencing of YB-1–associated RNAs. (D) In a normal prostate epithelial cell line (RWPE), YB-1 expression is less than that of the prostate cancer cell line (PC-3). Shad1 level is also down-regulated in RWPE compared with PC-3. (E) Upon lentiviral infection with shRNA for YB-1, Shad1 levels also decrease. (F) Upon overexpression of YB-1, Shad1 levels are also increased. (*) P-values <0.05; n = 3.