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. 2015 Jun;21(6):1173–1186. doi: 10.1261/rna.050583.115

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© 2015 Schwer and Shuman; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 8. — Genetic interactions of Snp1 amino-terminal truncations. (Top) Alignment of the amino-terminal amino acid sequences of the S. cerevisiae Snp1 and human U1-70K. Positions of side chain identity/similarity are indicated by • above the alignment. Arrowheads indicate the boundaries of the ▵N truncations of Snp1. (Bottom) Yeast snp1▵ strains bearing the indicated SNP1-▵N allele on a CEN HIS3 plasmid in an otherwise wild-type (top panel), mud2▵, mud1▵, nam8▵, or tgs1▵ background as indicated were spot-tested for growth on YPD agar at the temperatures specified. Synthetic growth defects are denoted by •.