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. 1994 Jul 19;91(15):7212–7216. doi: 10.1073/pnas.91.15.7212

KAR3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore.

K Middleton 1, J Carbon 1
PMCID: PMC44369  PMID: 8041770

Abstract

We have used in vitro motility assays to investigate the mechanism of kinetochore function in the budding yeast Saccharomyces cerevisiae. Functional centromeric DNA plus a tripartite centromere binding protein complex, CBF3, was found to be necessary but not sufficient for in vitro kinetochore activity. A fourth required component was identified as the motor protein Kar3p, a previously reported yeast kinesin known to be involved in karyogamy and mitosis. Our data support genetic evidence suggesting that Kar3p is a kinetochore-associated motor and imply that CBF3 plays a regulatory role in kinetochore function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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