In the article “Regulation of Postsynaptic Function by the Dementia-Related ESCRT-III Subunit CHMP2B” by Romain Chassefeyre, José Martínez-Hernández, Federica Bertaso, Nathalie Bouquier, Béatrice Blot, Marine Laporte, Sandrine Fraboulet, Yohann Couté, Anny Devoy, Adrian M. Isaacs, Karin Pernet-Gallay, Rémy Sadoul, Laurent Fagni, and Yves Goldberg, which appeared on pages 3155–3173 of the February 18, 2015 issue, in the Materials and Methods section, it was erroneously stated that the live imaging medium contained 2.5 mm KCl. The actual concentration was 5 mm KCl. This correction does not affect the interpretation of the data. In addition, in Table 4, an error occurred in the proof production stage. The column reporting the specific spectral counts (SSC) of proteins immunoprecipitated with Chmp2b IgG was inadvertently shifted under the title “Control IgG.” The corrected Table 4 appears below. We apologize for any confusion these errors may have caused.
Table 4.
Complete list of proteins detected by mass spectrometry of the synaptic Chmp2b-cotaining HMW complex
| Protein name | Accession number | Note | SC 2B/SC control | Chmp2b IgG |
Control IgG |
||
|---|---|---|---|---|---|---|---|
| SC | SSC | SC | SSC | ||||
| Bag6 | BAG6_RAT | 2B only | 35 | 35 | |||
| Gja1 | CXA1_RAT | 2B only | 29 | 29 | |||
| Get4 | F1LXF5_RAT | 2B only | 23 | 23 | |||
| Camk2g | KCC2G_RAT | PSD | 2B only | 14 | 4 | ||
| Chmp4b | D4A9Z8_RAT | ESCRT-III | 2B only | 12 | 12 | ||
| Fasn | FAS_RAT | 2B only | 11 | 11 | |||
| Ptpn23 | F1M951_RAT | ESCRT-III | 2B only | 10 | 10 | ||
| Alix | PDC6I_RAT | ESCRT-III | 2B only | 10 | 10 | ||
| Ubl4a | UBL4A_RAT | 2B only | 10 | 10 | |||
| Wdr7 | WDR7_RAT | 2B only | 10 | 10 | |||
| Myo6 | D4A5I9_RAT | CSK | 2B only | 9 | 9 | ||
| Hk1 | HXK1_RAT | 2B only | 9 | 9 | |||
| Sptbn2 | SPTN2_RAT | CSK | 2B only | 9 | 7 | ||
| Chmp2a | B2RZB5_RAT | ESCRT-III | 2B only | 8 | 8 | ||
| Chmp3 | CHMP3_RAT | ESCRT-III | 2B only | 8 | 8 | ||
| Pfkp | K6PP_RAT | 2B only | 8 | 7 | |||
| Ogt | OGT1_RAT | 2B only | 8 | 8 | |||
| Pfkm | Q52KS1_RAT | 2B only | 8 | 7 | |||
| Srcin1 | SRCN1_RAT | 2B only | 8 | 8 | |||
| Myo1d | MYO1D_RAT | CSK | 2B only | 7 | 7 | ||
| Capzb | CAPZB_RAT | CSK | 2B only | 6 | 6 | ||
| Myh14 | F1LNF0_RAT | CSK | 2B only | 6 | 2 | ||
| Ank2 | F1M9N9_RAT | CSK | 2B only | 6 | 6 | ||
| Sfxn | G3V804_RAT | 2B only | 6 | 2 | |||
| Homer1 | HOME1_RAT | PSD | 2B only | 6 | 6 | ||
| Ppp1ca | PP1A_RAT | 2B only | 6 | 2 | |||
| Ppp1cc | PP1G_RAT | 2B only | 6 | 2 | |||
| Hspa12a | D3ZC55_RAT | 2B only | 5 | 5 | |||
| Dbn1 | DREB_RAT | PSD | 2B only | 5 | 5 | ||
| Gphn | GEPH_RAT | 2B only | 5 | 5 | |||
| Idh2 | IDHP_RAT | 2B only | 5 | 5 | |||
| Shank1 | SHAN1_RAT | PSD | 2B only | 5 | 5 | ||
| Tpi1 | TPIS_RAT | 2B only | 5 | 5 | |||
| Sptbn1 | G3V6S0_RAT | CSK | 35.0 | 35 | 33 | (1) | (1) |
| Myh10 | F1LQ02_RAT | CSK | 24.0 | 24 | 19 | (1) | (1) |
| Dync1h1 | DYHC1_RAT | 22.0 | 22 | 5 | (1) | (1) | |
| Ap3d1 | B5DFK6_RAT | 12.0 | 12 | 12 | (1) | (1) | |
| Myh9 | G3V6P7_RAT | CSK | 11.0 | 11 | 6 | (1) | (1) |
| Cyfip2 | D3ZX82_RAT | CSK | 2B only | 4 | 4 | ||
| Chmp1a | D4AE79_RAT | ESCRT-III | 2B only | 4 | 4 | ||
| Glud1 | DHE3_RAT | 2B only | 4 | 4 | |||
| Sucla2 | F1LM47_RAT | 2B only | 4 | 4 | |||
| Epb41l3 | G3V874_RAT | CSK | 2B only | 4 | 4 | ||
| Kif2a | KIF2A_RAT | 2B only | 4 | 4 | |||
| Pclo | PCLO_RAT | 2B only | 4 | 4 | |||
| Snf8 | SNF8_RAT | 2B only | 4 | 4 | |||
| Snap25 | SNP25_RAT | 2B only | 4 | 4 | |||
| Actn1 | ACTN1_RAT | CSK | 2B only | 3 | 3 | ||
| Agap2 | AGAP2_RAT | 2B only | 3 | 3 | |||
| Snap91 | AP180_RAT | 2B only | 3 | 3 | |||
| Atp5f1 | AT5F1_RAT | 2B only | 3 | 3 | |||
| LOC681996 | B0BN63_RAT | 2B only | 3 | 3 | |||
| Arpc4 | B2RZ72_RAT | CSK | 2B only | 3 | 3 | ||
| Ndufa4 | B2RZD6_RAT | 2B only | 3 | 3 | |||
| Capza2 | CAZA2_RAT | CSK | 2B only | 3 | 3 | ||
| Chmp5 | CHMP5_RAT | ESCRT-III | 2B only | 3 | 3 | ||
| Rapgef4 | D3KR63_RAT | 2B only | 3 | 3 | |||
| Chmp6 | D3ZDR2_RAT | ESCRT-III | 2B only | 3 | 3 | ||
| Dclk1 | DCLK1_RAT | 2B only | 3 | 3 | |||
| Dlg4 | DLG4_RAT | PSD | 2B only | 3 | 3 | ||
| Dynll1 | DYL1_RAT | 2B only | 3 | 3 | |||
| H2a1c | H2A1C_RAT | 2B only | 3 | 3 | |||
| Prkar2b | KAP3_RAT | 2B only | 3 | 3 | |||
| Prkcg | KPCG_RAT | 2B only | 3 | 3 | |||
| Nckap1 | NCKP1_RAT | CSK | 2B only | 3 | 3 | ||
| Ndufs2 | NDUS2_RAT | 2B only | 3 | 3 | |||
| Ndufv2 | NDUV2_RAT | 2B only | 3 | 3 | |||
| Plec | PLEC_RAT | 2B only | 3 | 3 | |||
| Pura | PURA_RAT | 2B only | 3 | 3 | |||
| Pygb | PYGB_RAT | 2B only | 3 | 3 | |||
| Syt7 | Q99P34_RAT | 2B only | 3 | 3 | |||
| Rab2a | RAB2A_RAT | 2B only | 3 | 3 | |||
| Ran | RAN_RAT | 2B only | 3 | 3 | |||
| Dnm1 | DYN1_RAT | 8.7 | 26 | 26 | 3 | 3 | |
| Atp2b1 | AT2B1_RAT | 8.0 | 8 | 3 | (1) | (1) | |
| Nsf | NSF_RAT | 8.0 | 16 | 16 | 2 | 2 | |
| Rab3c | RAB3C_RAT | 8.0 | 8 | 4 | (1) | (1) | |
| Atp2b2 | AT2B2_RAT | 7.0 | 7 | 3 | (1) | (1) | |
| Rab14 | RAB14_RAT | 7.0 | 7 | 3 | (1) | (1) | |
| Rims1 | RIMS1_RAT | 7.0 | 7 | 7 | (1) | (1) | |
| Syn2 | SYN2_RAT | 7.0 | 7 | 4 | (1) | (1) | |
| Add2 | ADDB_RAT | CSK | 5.0 | 5 | 4 | (1) | (1) |
| Atp5o | ATPO_RAT | 5.0 | 5 | 5 | (1) | (1) | |
| RGD1307615 | D3ZKQ4_RAT | 5.0 | 5 | 5 | (1) | (1) | |
| Sptan1 | E9PSZ3_RAT | CSK | 5.0 | 35 | 35 | 7 | 7 |
| Immt | IMMT_RAT | 5.0 | 5 | 5 | (1) | (1) | |
| Camk2b | KCC2B_RAT | PSD | 5.0 | 30 | 13 | 6 | (1) |
| Aco2 | ACON_RAT | 2B only | 2 | 2 | |||
| Maoa | AOFA_RAT | 2B only | 2 | 2 | |||
| Ap3m2 | AP3M2_RAT | 2B only | 2 | 2 | |||
| Actr2 | ARP2_RAT | CSK | 2B only | 2 | 2 | ||
| Arpc5l | ARP5L_RAT | CSK | 2B only | 2 | 2 | ||
| Atad3 | ATAD3_RAT | 2B only | 2 | 2 | |||
| Cacna1a | CAC1A_RAT | 2B only | 2 | 2 | |||
| Cand1 | CAND1_RAT | 2B only | 2 | 2 | |||
| Crym | CRYM_RAT | 2B only | 2 | 2 | |||
| Caskin1 | CSKI1_RAT | 2B only | 2 | 2 | |||
| Atp5j2 | D3ZAF6_RAT | 2B only | 2 | 2 | |||
| Dsg1b | D3ZQ45_RAT | 2B only | 2 | 2 | |||
| Atp6v1a | D4A133_RAT | 2B only | 2 | 2 | |||
| Add3 | D4A3Q7_RAT | CSK | 2B only | 2 | 2 | ||
| Epb41l1 | E41L1_RAT | CSK | 2B only | 2 | 2 | ||
| Hadha | ECHA_RAT | 2B only | 2 | 2 | |||
| Ank3 | F1LP19_RAT | CSK | 2B only | 2 | 2 | ||
| Mrpl48 | F1LWB8_RAT | 2B only | 2 | 2 | |||
| Chmp2b | F1M8B7_RAT | ESCRT-III | 2B only | 2 | 2 | ||
| Gnb1 | GBB1_RAT | 2B only | 2 | 2 | |||
| Slc2a1 | GTR1_RAT | 2B only | 2 | 2 | |||
| Eif4a2 | IF4A2_RAT | 2B only | 2 | 2 | |||
| Kcnh1 | KCNH1_RAT | 2B only | 2 | 2 | |||
| Prkcb | KPCB_RAT | 2B only | 2 | 2 | |||
| Ldha | LDHA_RAT | 2B only | 2 | 2 | |||
| Mink1 | MINK1_RAT | 2B only | 2 | 2 | |||
| Grin2b | NMDE2_RAT | PSD | 2B only | 2 | 2 | ||
| Pdhb | ODPB_RAT | 2B only | 2 | 2 | |||
| Pde2a | PDE2A_RAT | 2B only | 2 | 2 | |||
| Mrps9 | Q5I0K4_RAT | 2B only | 2 | 2 | |||
| Ndufs7 | Q5RJN0_RAT | 2B only | 2 | 2 | |||
| Ndufv1 | Q5XIH3_RAT | 2B only | 2 | 2 | |||
| Uqcrc2 | QCR2_RAT | 2B only | 2 | 2 | |||
| Rab18 | RAB18_RAT | 2B only | 2 | 2 | |||
| Rab7a | RAB7A_RAT | 2B only | 2 | 2 | |||
| Rac1 | RAC1_RAT | 2B only | 2 | 2 | |||
| Mras | RASM_RAT | 2B only | 2 | 2 | |||
| Rpl23a | RL23A_RAT | 2B only | 2 | 2 | |||
| Rtn1 | RTN1_RAT | 2B only | 2 | 2 | |||
| Sept5 | SEPT5_RAT | CSK | 2B only | 2 | 2 | ||
| Sipa1l1 | SI1L1_RAT | 2B only | 2 | 2 | |||
| Ssbp1 | SSBP_RAT | 2B only | 2 | 2 | |||
| Stx1b | STX1B_RAT | 2B only | 2 | 2 | |||