Serum level of eight cytokines in Han Chinese patients with systemic lupus erythematosus using multiplex fluorescent microsphere method

Abstract

Aim of the study

To investigate the role of 8 cytokines and their correlation with clinical characteristics in systemic lupus erythematosus (SLE) in Han Chinese population by detecting their serum levels using the multiplex fluorescent microsphere method.

Material and methods

Serum was separated from 79 patients with SLE and 40 healthy controls. The serum cytokine detection was conducted according to the instruction of MILLIPLEX MAP human cytokine detection kit on the Luminex liquid phase array platform with 0.01 pg/ml detectable level. The 8 cytokines were interferon α2 (IFN-α2), IFN-γ, interleukin 6 (IL-6), IL-8, IL-10, IFN-γ-inducible protein 10 (IP-10), tumor necrosis factor α (TNF-α) and IL-17. Variable data were in skewed distribution and were expressed with median (P25, P75). Mann-Whitney analysis was used in statistical analysis.

Results

At the baseline level without any stimulus, the level of IP-10 expression was the highest among the 8 cytokines and the second highest was IL-8. The level of IL-17 was too low to be detected. The level of 7 cytokines was higher in SLE patients than in healthy controls (p < 0.01). The level of dsDNA antibody, C3, CRP, ESR and anti-nucleosome antibody was correlated with IL-10. Proteinuria was not correlated with any cytokine.

Conclusions

Eight cytokines were measured in our study, while not all of them were detected. The most important finding was the usefulness of IL-10 as a disease activity biomarker for Han Chinese patient with SLE. None of cytokines reflected kidney injury.

Introduction

Systemic lupus erythematosus (SLE) is a common autoimmune disease, and the main feature is activation of autoreactive T cells and B cells, which causes production of a large amount of autoantibodies and increase in circulating immune complexes. Up to now, the pathogenesis of autoimmunity in SLE has still not been very clear. There is strong evidence supporting the role of cytokines in the pathogenesis of SLE. Complex interplay between Th1 and Th2-type cytokines is involved and the study showed Th1/ Th2 cytokine profile imbalance existed in SLE patients [1]. Cytokines are soluble factors which are mostly generated by immune cells and in turn play crucial roles in the differentiation, maturation, and activation of various immune cells. Abnormal release or functions of diverse cytokines have been identified in SLE. Some cytokines, including IL-10, may exert pro- or anti-inflammatory activity, depending on target cell type (e.g. IL-10 inhibits T cell activity but enhances humoral response), hence it is unfavorable in SLE [2].

Currently, the treatment for SLE has evolved from conventional drugs such as corticosteroids and immunosuppressants to biological-target therapies, in which cytokines are the most important therapeutic targets. Knowledge about Tregs and regulatory cytokines would not only provide new insights into the pathogenesis of SLE but could be also used to develop various clinical applications.

The concentrations of 8 cytokines including interferon α2 (IFN-α2), IFN-γ, interleukin 6 (IL-6), IL-8, IL-10, IFN-γ-inducible protein 10 (IP-10), tumor necrosis factor α (TNF-α) and IL-17 were measured in sera of SLE patients using multiplex fluorescent microsphere method in our study. In addition, the relationship between cytokine concentrations and clinical characteristics such as double-stranded DNA (dsDNA) antibody, anti-nucleosome antibody (AnuA), complement components (C3, C4), proteinuria and SLE disease activity index (SLEDAI) [3] was assessed by Spearman's correlation test.

Material and methods

Subjects

Seventy-nine patients (76 women and 3 men) aged 34.41 ±9.41 years with SLE were enrolled in our study. The patients were hospitalized in our hospital from 2011 to 2012 . The diagnosis was made according to 1997 American College of Rheumatology guideline. 40 healthy donors (35 women and 5 men) aged 37.65 ±14.82 years were recruited from the outpatient clinic, excluding subjects suffering from hypertension, heart disease, diabetes and autoimmune diseases. All the individuals signed the written consent form and the study was approved by the local ethics committee of the hospital.

Clinical features

Clinical characteristics including dsDNA antibody, AnuA, C3, C4, proteinuria and SLEDAI index were used to assess the disease activity. All patients received standard treatment including mainly steroids and immunosuppressant at different dosage according to their disease activity and degree of organ damage.

Cytokine detection

Five hundred μl of serum was taken from every individual and stored at –80°C. MILLIPLEX MAP human cytokine detection kit was purchased from Millipore Corporation [4]. Measurement of serum cytokine concentrations was conducted according to the instructions. Seven standard samples were set and 2 quality controls, standard sample and detection samples were all assayed in triplicate. The signal value was detected on Luminex chip platform and serum concentration was calculated according to the standard curve. The concentration range was 0-10 000 pg/ml for all 8 cytokines and the sensitivity of detection achieved 0.01 pg/ml level.

Statistical analysis

Data analysis was conducted by SPSS18.0 software package. For data in skewed distribution, the serum concentration of cytokines was expressed as median (P25, P75). Non-parametric Mann-Whitney U test was used for statisitical analysis. Correlation coefficient was measured using Spearman's correlation test. P < 0.05 was considered to be statistically significant.

Results

Demographic and clinical characteristics of patients and controls included in the study are shown in Table 1. The concentration of IP-10 expression was the highest among the 8 cytokines followed by IL-8. The concentration of IL-17 was too low to be detected. This cytokine was only detected in 7 of 79 SLE patients at the highest concentration of 3 pg/ml and in one healthy donor at a concentration of 1.26 pg/ml. Relatively low concentrations of IFN-α2, IFN-γ, IL-6, IL-10 and TNF-α were detected in sera but all of them were higher in SLE patients than that in healthy donors (p < 0.01). Similarly, concentrations of IL-8 and IP-10 were higher in SLE patients than in healthy donors (p < 0.01) (Table 2). Also IL-17 was correlated with some (IFN-γ, IL-10 and IL-6) but not all cytokines (Table 3). There was a positive correlation between serum concentrations of the majority of tested cytokines, except IFN-α2 and IL-8.

Table 1.

Demographic and clinical characteristics of patients and healthy donors included in the study

	SLE (n = 79)	Healthy donors (n = 40)	P values
Age in years (mean)	34.41 ±9.41	37.65 ±14.82	0.212
Female:male	76:3	35:5	0.117
Disease duration (yrs)	6.91 ±6.19	–
Age at onset (yrs)	27.36 ±7.67	–
ANA > 1:320	74/75	–
anti-dsDNA antibody positivity	32/78	–
anti-AnuA positivity	26/63	–
anti-SmD1 positivity	39/76	–
Low C4	72/79	–
Low C3	48/79	–
proteinuria	22/60	–
SLEDAI score	Inactive (0-4): 18/77 Mild active (5-9): 37/77 Median active (10-14): 16/77 Severe active (>14): 6/77	–

ANA – anti-nuclear antibody; dsDNA – double-stranded DNA; AnuA – anti-nucleosome antibody; anti-SmD1 – antibody for D1 protein of the Sm proteins; SLEDAI – systemic lupus erythematosus disease activity index

Table 2.

The serum level of cytokines in patients with SLE and healthy donors expressed as median [(P25, P75), pg/ml]

Cytokine	Group		Z value	P value
	Patients with SLE (n = 79)	Healthy donors (n = 40)
IFN-α2	0 (0, 1.28)	0 (0,0)	–3.417	0.001
IFN-γ	0.96 (0, 2.13)	0 (0,0.53)	–4.176	< 0.001
IL-6	0.64 (0,4.17)	0 (0,0)	–4.627	< 0.001
IL-8	357.99 (142.54, 934.70)	149.41 (73.10, 269.96)	–3.645	< 0.001
IL-10	1.19 (0, 6.05)	0(0,0)	–5.594	< 0.001
IP-10	1191.39 (754.73, 1979.67)	541.28 (446.29, 825.53)	–5.327	< 0.001
TNF-α	7.97 (1.41, 15.13)	2.21 (0.75, 4.45)	–4.655	< 0.001
IL-17	0 (0,0)	0 (0,0)	–1.442	0.149

Data are given as medians and 25-75% range.

Table 3.

Correlation between concentration of cytokines presented as r and p value

		IFN-α2	IFN-γ	IL-6	IL-8	IL-10	IP-10	TNF-α	IL-17
IFN-α2	r p	–	0.048 0.674	0.07 0.532	–0.033 0.770	0.065 0.566	0.091 0.425	0.163 0.152	–0.048 0.672
IFN-γ	r p	0.048 0.674	–	0.664 0.000	0.131 0.250	0.331 0.003	0.332 0.003	0.663 0.000	0.291 0.009
IL-6	r p	0.071 0.532	0.664 0.000	–	0.020 0.863	0.476 0.000	0.257 0.022	0.746 0.000	0.332 0.003
IL-8	r p	–0.033 0.770	0.131 0.250	0.020 0.863	–	–0.072 0.527	–0.079 0.491	0.080 0.481	–0.005 0.963
IL-10	r p	0.065 0.566	0.331 0.003	0.476 0.000	–0.072 0.527	–	0.390 0.000	0.457 0.000	0.249 0.027
IP-10	r p	0.091 0.425	0.332 0.003	0.257 0.022	–0.079 0.491	0.390 0.000	–	0.256 0.023	0.004 0.974
TNF-α	r p	0.163 0.152	0.663 0.000	0.746 0.000	0.080 0.481	0.457 0.000	0.256 0.023	–	0.148 0.192
IL-17	r p	–0.048 0.672	0.291 0.009	0.332 0.003	–0.005 0.963	0.249 0.027	0.004 0.974	0.148 0.192	–

P < 0.05 was considered as statistically significant.

Among tested cytokines only IL-10 positively correlated with the majority of clinical indices, including SLEDAI, systemic inflammation markers (CRP, ESR), and autoantibody titres. Consistently, there was also an inverse correlation between this cytokine and serum concentration of C3, known to be reciprocally associated with the disease activity (Table 4, Fig. 2). Interestingly, also other cytokines (IL-6, TNF, IFN, and IP-10) correlated with systemic inflammation markers (CRP and/or ESR) and the concentration of at least one autoantibody (anti-dsDNA and/or AnuA). By contrast, IL-17 correlated positively only with the concentrations of complement components (C3, C4) (Table 4). However, this finding is uncertain because IL-17 was detected merely in 8.8% of SLE patients.

Table 4.

Correlation between concentration of cytokines and clinical parameters presented as r and p value

		C3	C4	CRP	ESR	dsDNA	AnuA	proteinuria	SLEDAI
IFN-α2	r p	–0.154 0.175	–0.101 0.378	–0.113 0.329	0.183 0.126	0.206 0.071	0.171 0.176	–0.037 0.777	0.129 0.264
IFN-γ	r p	0.052 0.648	0.203 0.073	0.226 0.050	0.262 0.028	0.210 0.065	0.305 0.014	0.017 0.899	–0.139 0.226
IL-6	r p	–0.064 0.578	–0.005 0.965	0.423 0.000	0.427 0.000	0.352 0.002	0.315 0.011	0.009 0.944	–0.065 0.574
IL-8	r p	0.143 0.208	0.211 0.062	0.034 0.769	–0.259 0.029	0.006 0.956	–0.080 0.529	–0.101 0.443	–0.127 0.270
IL-10	r p	–0.271 0.016	–0.221 0.073	0.373 0.001	0.532 0.000	0.298 0.008	0.457 0.000	0.241 0.064	0.333 0.003
IP-10	r p	–0.157 0.167	–0.195 0.085	0.207 0.073	0.387 0.001	0.141 0.219	0.424 0.000	–0.029 0.828	0.141 0.221
TNF-α	r p	–0.135 0.236	–0.053 0.642	0.232 0.044	0.387 0.001	0.282 0.012	0.365 0.003	0.061 0.643	0.035 0.764
IL-17	r p	0.234 0.038	0.222 0.049	0.180 0.120	0.088 0.463	0.154 0.179	0.015 0.905	–0.049 0.712	–0.112 0.333

Data was presented as (r, p) values. P < 0.05 was considered as statistically significant. Clinical parameters included complements, autoantibodies, inflammation and clinical indices.

Fig. 2.

Correlation between IL-10 concentration and clinical indices in SLE patients. X-axis represents serum concentration of IL-10 in SLE patients. Y-axis represents clinical indices including quantity of C3, C4, CRP, ESR, dsDNA, AnuA, proteinuria and SLEDAI scoring in corresponding individuals. IL-10 concentration was correlated with all clincial indexes in SLE patients as shown by the scatter dot plot

Thus, in comparison with healthy volunteers, sera of SLE patients contained significantly higher concentrations of seven cytokines, including IFN-α2, IFN-γ, IL-6, IL-8, IL-10, IP-10, TNF-α (Fig. 1). Although several cytokines were moderately related to some clinical indices (Table 4), IL-10 showed the strongest association, because this cytokine correlated with almost all evaluated parameters (Fig. 2).

Fig. 1.

The serum concentration of cytokines in patients with SLE and healthy donors. X-axis represents the SLE group and healthy donors group, respectively. Y-axis represents the serum concentration of cytokines including IFN-α2, IFN-γ, IL-6, IL-8, IL-10, IP-10, TNF-α, IL-17, accordingly. P value was noted for differences of the serum concentration of cytokines between the two groups. P < 0.05 was considered to be statistically significant

Discussion

Numerous reports have characterised systemic and local cytokine profiles in SLE patients [5–8]. However, the results concerning certain cytokines remain controversial, for example, a significant negative correlation between the levels of IL-10 and SLEDAI scores was found in the northern Indian subjects [9]. Nonetheless, other studies [10–12] reported elevated levels of serum IL-10 in SLE patients which correlated well with SLE disease activity. Several studies have found increased serum levels of IFN-γ [13–18] and TNF-α [14, 19–22] in patients with active stages of SLE. Other studies have found IFN-γ [23–25] and TNF-α [23] levels unaltered in the disease course. Possible explanations of this discrepancy are application of cytokine measurement techniques of different sensitivity, small patients cohort and different ethinity.

Compared with former studies in northern Indian subjects [9], our study might be more convincing due to a larger sample size recruited. Besides, a high resolution approach was applied to detect the cytokines in human specimens in our study. It would be more convenient to detect multiple cytokines simultaneously, while detecting cytokine concentration using ELISA once at a time might probably raise interference. Another advantage was the trifle requirement of serum of only 50 μl in our study. Usually, 200 μl of serum would be needed in ELISA assay. Therefore, more blood should be taken from patients when detecting several cytokines by ELISA approach.

The results of our study are consistent with some former studies [26–28]. We have found seven cytokines, including IFN-α2, IFN-γ, IL-6, IL-8, IL-10, IP-10, TNF-α to be overproduced in SLE patients, because serum concentrations of these cytokines were significantly higher than in healthy donors. Despite this, only IL-10 correlated with all disease activity indices.

Numerous studies have demonstrated that serum IL-10 concentration is significantly elevated and correlates with lupus disease activity [10–12]. The correlation was replicated in our Chinese Han population. In murine lupus IL-10 plays a pathologic role, for continuous therapy from the young age with IL-10 antibodies ameliorated autoimmunity in NZB/W F1 mice [29]. In accordance with the therapeutic effect of anti-IL-10 antibodies, the continuous administration of recombinant IL-10 increased disease activity. Similarly, in a small, uncontrolled, open-label study involving patients with mild disease, anti-IL-10 monoclonal antibody improved cutaneous lesions, joint symptoms, and the SLE disease activity index [30].

In addition, IL-10 concentration in the exhaled breath condensate (EBC) and bronchoalveolar lavage fluid (BALF) was higher in patients with SLE compared with healthy donors. Thus, the measurement of IL-10 in the EBC may be a useful biomarker of SLE activity. It is likely that IL-10 protects against pulmonary manifestations of SLE [31].

Although other cytokines present in SLE patients sera in elevated concentrations showed a more limited correlation with clinical indices, they may also contribute to development of various disease symptoms, e.g. lupus nephritis (LN).

The expression of IL-6 is increased in the kidneys of patients with active lupus nephritis and has been shown to contribute to disease pathogenesis. Measurement of the IL-6 concentration in SLE patients could help to predict future renal involvement in SLE patients [32].

Numerous studies have defined the cytokine and chemokine profiles in the cerebrospinal fluid (CSF) from patients with NPSLE. Patients with headache had increased CSF concentrations of IL-6, IL-8 and IP-10 compared with non-NPSLE and non-autoimmune diseases patients [5–7].

Patients with severe/extensive skin lesions showed a higher frequency of cytokine gene overexpression. An increased IFN-γ expression suggests its involvement in SLE skin inflammation [8].

Recently, the role of IL-23/IL-17 axis in SLE has been studied as well. Significantly higher levels of glomerular IL-17 and IL-23 expression were observed in renal biopsies from class IV LN patients as compared to those from MCN patients and normal controls. Glomerular IL-17 and IL-23 expression levels were positively correlated with renal SLEDAI and histological activity index for LN patients. These results suggest the potential role of the IL-23/ Th17 axis in the intra-renal inflammation of SLE. Serum IL-17 concentration correlates poorly with SLE disease activity, but is significantly elevated in patients with CNS disease [33–35].

Lupus nephritis is one of the most common and serious manifestations of SLE. In our cohort, nearly 55.84% (43/77) of patients had either previous or current LN. Patients were considered to have active renal disease if proteinuria was above 0.5 mg/day, hematuria was above 5 red blood cells per high-power field, pyuria was above 5 white blood cells/high-power field, or cellular casts were present. Unfortunately, none of tested cytokines is correlated with renal impairment in our study. Compared with kidney tissue biopsy, cytokine from serum source are probably not an ideal approach for assessment of LN involvement.

IP-10 was repeatedly reported to be correlated with disease activity and clinical manifestations in SLE [36, 37]. However, we failed to show such association in our study. We found this cytokine to be correlated with ESR value and AnuA concentration only (Table 4). This discrepancy may result from distinct population diversity. Further investigation of this cytokine is needed to confirm this supposition.

In conclusion, IL-10 was elevated in Han Chinese patients with SLE and might be used as a serum marker to evaluate disease activity.

The authors declare no conflict of interest.

This research was supported by National grants (81102266, 81301529) and the Shenzhen Key Discipline Foundation (2005C10).