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Effect of metal ions on the internalization of BLM-Cy5** conjugates in four sets of matched cancer and normal cell lines. The cells were treated with 25 μM Cu(II)•BLM-Cy5**, Fe(III)•BLM-Cy5**, Mn(III)•BLM-Cy5**, or Zn(II)•BLM-Cy5** at 37 °C for 1 h, washed with PBS, and fixed with 4% paraformaldehyde. Fluorescence imaging was carried out with a 2 s exposure time.
