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. 1972 Mar;1(3):247–251. doi: 10.1128/aac.1.3.247

Incorporation of Labeled Precursors into A16886B, a Novel β-Lactam Antibiotic Produced by Streptomyces clavuligerus

J G Whitney 1, D R Brannon 1, J A Mabe 1, K J Wicker 1
PMCID: PMC444200  PMID: 5045471

Abstract

The incorporation of C-14 from amino acids into A16886B, 7-(5-amino-5-carboxyvaleramido)-7-methoxy-3-carbamoyloxymethyl-3-cephem-4- carboxylic acid, by Streptomyces clavuligerus is reported. As with cephalosporin C biosynthesis by the mold Cephalosporium acremonium, labels from cysteine, valine, and α-amino-adipic acid were incorporated. Unlike cephalosporin C, in A16886B label from lysine was incorporated into the α-aminoadipic acid side chain. Label from methionine-14C-methyl was incorporated into the methoxyl group. The relative percentage of incorporation of 3H and 14C from doubly labeled cystine suggests the improbability of the C-7 methoxyl group arising from an intermediate containing a double bond between C-6 and C-7.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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