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. 1972 May;1(5):367–372. doi: 10.1128/aac.1.5.367

Evaluation of an Experimental Animal Model for Testing Antifungal Substances

M Huppert 1,2,3, S H Sun 1,2,3, A J Gross 1,2,3,1
PMCID: PMC444225  PMID: 4670475

Abstract

Accumulated evidence indicates that infection by fungi capable of causing systemic disease usually results in a relatively strong acquired resistance. The working hypothesis for this study was that an antifungal substance, even one with only slight fungistatic activity, could be an effective chemotherapeutic agent by arresting progression of the infection until acquired resistance became effective. The present study involved establishing and evaluating an experimental animal model (coccidioidomycosis in mice) which could be used to test this hypothesis. This model was reasonably similar to the natural disease. Results with infected nontreated animals indicated that the plot of mortality frequency against survival time did not follow a normal distribution curve. Thus, nonparametric procedures were used for evaluation. Use of this model with an established antibiotic (amphotericin B), with a crude preparation of a new antibiotic (CB-310), and with synthetic organoselenium compounds demonstrated that even low levels of antifungal activity could be detected. The model should be useful not only to test the original hypothesis but also to screen antifungal substances for their potential as chemotherapeutic agents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FRIEDMAN L., PAPAGIANIS D., BERMAN R. J., SMITH C. E. Studies on Coccidioides immitis: morphology and sporulation capacity of forty-seven strains. J Lab Clin Med. 1953 Sep;42(3):438–444. [PubMed] [Google Scholar]
  2. GEHAN E. A. A GENERALIZED WILCOXON TEST FOR COMPARING ARBITRARILY SINGLY-CENSORED SAMPLES. Biometrika. 1965 Jun;52:203–223. [PubMed] [Google Scholar]
  3. Kong Y. C., Levine H. B. Experimentally induced immunity in the mycoses. Bacteriol Rev. 1967 Mar;31(1):35–53. doi: 10.1128/br.31.1.35-53.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LEVINE H. B., KONG Y. C., SMITH C. IMMUNIZATION OF MICE TO COCCIDIOIDES IMMITIS: DOSE, REGIMEN AND SPHERULATION STAGE OF KILLED SPHERULE VACCINES. J Immunol. 1965 Jan;94:132–142. [PubMed] [Google Scholar]
  5. LOURIA D. B., FEDER N., EMMONS C. W. Amphotericin B in experimental histoplasmosis and Cryptococcosis. Antibiot Annu. 1956:870–877. [PubMed] [Google Scholar]
  6. Levine H. B., Kong Y. M. Immunity development in mice RECEIVING KILLED Coccidioides immitis spherules: effect of removing residual vaccine. Sabouraudia. 1965 Oct;4(3):164–170. [PubMed] [Google Scholar]
  7. OURA M., STERNBERG T. H., WRIGHT E. T. A new antifungal antibiotic, amphotericin B. Antibiot Annu. 1955;3:566–573. [PubMed] [Google Scholar]
  8. PINE L. Studies on the growth of Histoplasma capsulatum. II. Growth of the yeast phase on agar media. J Bacteriol. 1955 Oct;70(4):375–381. doi: 10.1128/jb.70.4.375-381.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. YOUMANS G. P., YOUMANS A. S. The measurement of the response of immunized mice to infection with Mycobacterium tuberculosis va. hominis. J Immunol. 1957 May;78(5):318–329. [PubMed] [Google Scholar]

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