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. 1972 Jun;1(6):508–511. doi: 10.1128/aac.1.6.508

Transduction of Tetracycline Resistance in Staphylococcus epidermidis

Barbara H Minshew 1, E D Rosenblum 1
PMCID: PMC444251  PMID: 4282454

Abstract

Fourteen Staphylococcus epidermidis strains were biotyped according to the scheme of Baird-Parker and tested for their sensitivities to S. epidermidis typing phages. Tetracycline-susceptible strains were tested for the capacity to be transduced to resistance. Five strains, all of biotype 1, were lysed by one or more phages. Five of eight biotype 1 strains were transduced to tetracycline resistance with rates of 10−8 to 10−5. Three strains of other biotypes were not transduced, nor was PS 73, an unusual coagulase-negative strain. The transducing phage φ367 was isolated from the lysogenic tetracycline-resistant donor strain. The phage morphologically resembles serological group B S. aureus phages. Restriction and modification were indicated from the efficiency of plating on phage-sensitive strains.

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Selected References

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