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. 1972 Sep;2(3):142–146. doi: 10.1128/aac.2.3.142

Phosphorylation of Lividomycin by Escherichia coli Carrying an R Factor

Masahito Yamaguchi 1, Tomoyuki Koshi 1, Fujio Kobayashi 1, Susumu Mitsuhashi 1
PMCID: PMC444281  PMID: 4597704

Abstract

A lividomycin-phosphorylating enzyme from a lividomycin-resistant strain of Escherichia coli carrying an R factor was partially purified by fractionation with ammonium sulfate and Sephadex G-100 column chromatography. The enzyme inactivated, in the presence of adenosine triphosphate and Mg2+, several antibiotics having a d-ribose moiety linked to 2-deoxystreptamine, i.e., lividomycin A and B, neomycin, paromomycin, and vistamycin, but did not inactivate the kanamycins, streptomycin, or the gentamicin C components. Chemical studies of the inactivated product suggested that the phosphorylated site of the inactivated lividomycin was the hydroxyl group of the d-ribose moiety.

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Selected References

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